<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering

The changes in histone acetylation mediated by histone deacetylases (HDAC) play a crucial role in plant development and response to environmental changes. Mammalian HDACs are regulated by post-translational modifications (PTM), such as phosphorylation, acetylation, ubiquitination and small ubiquitin...

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Autores principales:	Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HDA 6 SUMO E3 ligase SIZ1 flowering <i>Arabidopsis</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/031578a34acb4d499658902994e36e03
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id	oai:doaj.org-article:031578a34acb4d499658902994e36e03
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spelling	oai:doaj.org-article:031578a34acb4d499658902994e36e032021-11-25T17:10:18Z<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering10.3390/cells101130012073-4409https://doaj.org/article/031578a34acb4d499658902994e36e032021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3001https://doaj.org/toc/2073-4409The changes in histone acetylation mediated by histone deacetylases (HDAC) play a crucial role in plant development and response to environmental changes. Mammalian HDACs are regulated by post-translational modifications (PTM), such as phosphorylation, acetylation, ubiquitination and small ubiquitin-like modifier (SUMO) modification (SUMOylation), which affect enzymatic activity and transcriptional repression. Whether PTMs of plant HDACs alter their functions are largely unknown. In this study, we demonstrated that the <i>Arabidopsis</i> SUMO E3 ligase SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1) interacts with HISTONE DEACETYLASE 6 (HDA6) both in vitro and in vivo. Biochemical analyses indicated that HDA6 is not modified by SUMO1. Overexpression of <i>HDA6</i> in <i>siz1-3</i> background results in a decreased level of histone H3 acetylation, indicating that the activity of HDA6 is increased in <i>siz1-3</i> plants. Chromatin immunoprecipitation (ChIP) assays showed that SIZ1 represses HDA6 binding to its target genes <i>FLOWERING LOCUS C</i> (<i>FLC</i>) and <i>MADS AFFECTING FLOWERING 4</i> (<i>MAF4</i>), resulting in the upregulation of <i>FLC</i> and <i>MAF4</i> by increasing the level of histone H3 acetylation. Together, these findings indicate that the <i>Arabidopsis</i> SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates HDA6 function.Sujuan GaoXueqin ZengJianhao WangYingchao XuChunwei YuYishui HuangFeng WangKeqiang WuSongguang YangMDPI AGarticleHDA 6SUMO E3 ligase SIZ1flowering<i>Arabidopsis</i>Biology (General)QH301-705.5ENCells, Vol 10, Iss 3001, p 3001 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HDA 6 SUMO E3 ligase SIZ1 flowering <i>Arabidopsis</i> Biology (General) QH301-705.5
spellingShingle	HDA 6 SUMO E3 ligase SIZ1 flowering <i>Arabidopsis</i> Biology (General) QH301-705.5 Sujuan Gao Xueqin Zeng Jianhao Wang Yingchao Xu Chunwei Yu Yishui Huang Feng Wang Keqiang Wu Songguang Yang <i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
description	The changes in histone acetylation mediated by histone deacetylases (HDAC) play a crucial role in plant development and response to environmental changes. Mammalian HDACs are regulated by post-translational modifications (PTM), such as phosphorylation, acetylation, ubiquitination and small ubiquitin-like modifier (SUMO) modification (SUMOylation), which affect enzymatic activity and transcriptional repression. Whether PTMs of plant HDACs alter their functions are largely unknown. In this study, we demonstrated that the <i>Arabidopsis</i> SUMO E3 ligase SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1) interacts with HISTONE DEACETYLASE 6 (HDA6) both in vitro and in vivo. Biochemical analyses indicated that HDA6 is not modified by SUMO1. Overexpression of <i>HDA6</i> in <i>siz1-3</i> background results in a decreased level of histone H3 acetylation, indicating that the activity of HDA6 is increased in <i>siz1-3</i> plants. Chromatin immunoprecipitation (ChIP) assays showed that SIZ1 represses HDA6 binding to its target genes <i>FLOWERING LOCUS C</i> (<i>FLC</i>) and <i>MADS AFFECTING FLOWERING 4</i> (<i>MAF4</i>), resulting in the upregulation of <i>FLC</i> and <i>MAF4</i> by increasing the level of histone H3 acetylation. Together, these findings indicate that the <i>Arabidopsis</i> SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates HDA6 function.
format	article
author	Sujuan Gao Xueqin Zeng Jianhao Wang Yingchao Xu Chunwei Yu Yishui Huang Feng Wang Keqiang Wu Songguang Yang
author_facet	Sujuan Gao Xueqin Zeng Jianhao Wang Yingchao Xu Chunwei Yu Yishui Huang Feng Wang Keqiang Wu Songguang Yang
author_sort	Sujuan Gao
title	<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
title_short	<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
title_full	<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
title_fullStr	<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
title_full_unstemmed	<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
title_sort	<i>arabidopsis</i> sumo e3 ligase siz1 interacts with hda6 and negatively regulates hda6 function during flowering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/031578a34acb4d499658902994e36e03
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<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering

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