An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis

An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis

Di Zhang,1 Jinghong Yang,2 Chuang Liu,1 Sheng Ye,1 Qianbing Zhang,2 Ruiyuan Liu1 1Guangdong Provincial Key Laboratory of Medical Image Processing, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of China; 2Cancer Research Institute, Scho...

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Autores principales: Zhang D, Yang J, Liu C, Ye S, Zhang Q, Liu R
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2021
Materias:
nir absorbing chromophore
2-dicyanomethylenethiazole
photothermal therapy
dna damage induced apoptosis
rna-seq
and biological analysis
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/0316aacd00a84cb4955159752885ac65
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spelling oai:doaj.org-article:0316aacd00a84cb4955159752885ac652021-12-02T14:47:59ZAn Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis1178-2013https://doaj.org/article/0316aacd00a84cb4955159752885ac652021-07-01T00:00:00Zhttps://www.dovepress.com/an-acceptor--donor-structured-organic-chromophore-for-nir-triggered-th-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Di Zhang,1 Jinghong Yang,2 Chuang Liu,1 Sheng Ye,1 Qianbing Zhang,2 Ruiyuan Liu1 1Guangdong Provincial Key Laboratory of Medical Image Processing, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of China; 2Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of ChinaCorrespondence: Ruiyuan LiuGuangdong Provincial Key Laboratory of Medical Image Processing, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of ChinaTel/Fax +86-20-61647496Email ruiyliu@smu.edu.cnQianbing ZhangCancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of ChinaTel/Fax +86-20-61648378Email carl@smu.edu.cnIntroduction: It will be challenging to develop high-performance organic chromophores for light-triggered thermal ablation of the tumor. Besides, the mechanisms of organic chromophores for tumor therapy remain unclear. Herein, an acceptor-π-donor (A-π-D) structured organic chromophore based on 2-dicyanomethylenethiazole named PTM was developed for photothermal therapy (PTT) of tumors.Methods and Results: Biocompatible PTM nanoparticles (PTM NPs) were fabricated by enclosing PTM with Pluronic F-127. The results of optical and photothermal properties of PTM NPs showed robust near-infrared (NIR) absorption, excellent photostability and high photothermal conversion efficiency (56.9%). The results of flow cytometry, fluorescence microscopy, apoptosis, CCK-8 assays and animal experiments showed that PTM NPs had a good killing effect on tumors under NIR laser irradiation. Furthermore, mechanistic studies, RNA-seq and biological analysis revealed that PTM NPs can cause tumor cell death via DNA damage-mediated apoptosis.Conclusion: Light-induced thermal ablation effects of PTM NPs in vitro and vivo were surveyed. Collectively, our studies provided a new approach to developing a safe and effective photothermal agent for cancer treatment.Keywords: NIR absorbing chromophore, 2-dicyanomethylenethiazole, photothermal therapy, DNA damage induced apoptosis, RNA-seq, biological analysisZhang DYang JLiu CYe SZhang QLiu RDove Medical Pressarticlenir absorbing chromophore2-dicyanomethylenethiazolephotothermal therapydna damage induced apoptosisrna-seqand biological analysisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 4901-4911 (2021)
institution DOAJ
collection DOAJ
language EN
topic nir absorbing chromophore
2-dicyanomethylenethiazole
photothermal therapy
dna damage induced apoptosis
rna-seq
and biological analysis
Medicine (General)
R5-920
spellingShingle nir absorbing chromophore
2-dicyanomethylenethiazole
photothermal therapy
dna damage induced apoptosis
rna-seq
and biological analysis
Medicine (General)
R5-920
Zhang D
Yang J
Liu C
Ye S
Zhang Q
Liu R
An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis
description Di Zhang,1 Jinghong Yang,2 Chuang Liu,1 Sheng Ye,1 Qianbing Zhang,2 Ruiyuan Liu1 1Guangdong Provincial Key Laboratory of Medical Image Processing, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of China; 2Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of ChinaCorrespondence: Ruiyuan LiuGuangdong Provincial Key Laboratory of Medical Image Processing, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of ChinaTel/Fax +86-20-61647496Email ruiyliu@smu.edu.cnQianbing ZhangCancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of ChinaTel/Fax +86-20-61648378Email carl@smu.edu.cnIntroduction: It will be challenging to develop high-performance organic chromophores for light-triggered thermal ablation of the tumor. Besides, the mechanisms of organic chromophores for tumor therapy remain unclear. Herein, an acceptor-π-donor (A-π-D) structured organic chromophore based on 2-dicyanomethylenethiazole named PTM was developed for photothermal therapy (PTT) of tumors.Methods and Results: Biocompatible PTM nanoparticles (PTM NPs) were fabricated by enclosing PTM with Pluronic F-127. The results of optical and photothermal properties of PTM NPs showed robust near-infrared (NIR) absorption, excellent photostability and high photothermal conversion efficiency (56.9%). The results of flow cytometry, fluorescence microscopy, apoptosis, CCK-8 assays and animal experiments showed that PTM NPs had a good killing effect on tumors under NIR laser irradiation. Furthermore, mechanistic studies, RNA-seq and biological analysis revealed that PTM NPs can cause tumor cell death via DNA damage-mediated apoptosis.Conclusion: Light-induced thermal ablation effects of PTM NPs in vitro and vivo were surveyed. Collectively, our studies provided a new approach to developing a safe and effective photothermal agent for cancer treatment.Keywords: NIR absorbing chromophore, 2-dicyanomethylenethiazole, photothermal therapy, DNA damage induced apoptosis, RNA-seq, biological analysis
format article
author Zhang D
Yang J
Liu C
Ye S
Zhang Q
Liu R
author_facet Zhang D
Yang J
Liu C
Ye S
Zhang Q
Liu R
author_sort Zhang D
title An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis
title_short An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis
title_full An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis
title_fullStr An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis
title_full_unstemmed An Acceptor-π-Donor Structured Organic Chromophore for NIR Triggered Thermal Ablation of Tumor via DNA Damage-Mediated Apoptosis
title_sort acceptor-π-donor structured organic chromophore for nir triggered thermal ablation of tumor via dna damage-mediated apoptosis
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/0316aacd00a84cb4955159752885ac65
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