Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy

Photoconvertible proteins occupy two color channels thereby limiting multicolour localisation microscopy applications. Here the authors present UnaG, a new green-to-dark photoswitching fluorescent protein for super-resolution imaging, whose activation is based on a noncovalent binding with bilirubin...

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Autores principales: Jiwoong Kwon, Jong-Seok Park, Minsu Kang, Soobin Choi, Jumi Park, Gyeong Tae Kim, Changwook Lee, Sangwon Cha, Hyun-Woo Rhee, Sang-Hee Shim
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/0321226231404acaae779b9ffd021c0d
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Sumario:Photoconvertible proteins occupy two color channels thereby limiting multicolour localisation microscopy applications. Here the authors present UnaG, a new green-to-dark photoswitching fluorescent protein for super-resolution imaging, whose activation is based on a noncovalent binding with bilirubin.