Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina

Plant apoplast serves as the frontier battlefield of plant defense in response to different types of pathogens. Many pathogenesis-related (PR) proteins are accumulated in apoplastic space during the onset of plant–pathogen interaction, where they act to suppress pathogen infection. In this study, we...

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Autores principales:	Jiaojie Zhao, Weishuai Bi, Shuqing Zhao, Jun Su, Mengyu Li, Lisong Ma, Xiumei Yu, Xiaodong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	wheat pathogenesis-related protein lipid transfer protein apoplastic resistance Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0330d3f067954c5ba550c8328d51b4ce
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spelling	oai:doaj.org-article:0330d3f067954c5ba550c8328d51b4ce2021-12-01T02:44:31ZWheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina1664-462X10.3389/fpls.2021.771806https://doaj.org/article/0330d3f067954c5ba550c8328d51b4ce2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.771806/fullhttps://doaj.org/toc/1664-462XPlant apoplast serves as the frontier battlefield of plant defense in response to different types of pathogens. Many pathogenesis-related (PR) proteins are accumulated in apoplastic space during the onset of plant–pathogen interaction, where they act to suppress pathogen infection. In this study, we found the expression of Triticum aestivum lipid transfer protein 3 (TaLTP3) gene was unregulated during incompatible interaction mediated by leaf rust resistance genes Lr39/41 at the early infection stage. Stable transgenic wheat lines overexpressing TaLTP3 exhibited enhanced resistance to leaf rust pathogen Puccinia triticina. Transcriptome analysis revealed that overexpression of TaLTP3 specifically activated the transcription of pathogenesis-related protein 1a (TaPR1a) and multiple plant hormone pathways, including salicylic acid (SA), jasmonic acid (JA), and auxin, in response to the infection of the model bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Further investigation indicated that TaLTP3 physically associated with wheat TaPR1a protein in the apoplast. Transgenic wheat lines overexpressing TaLTP3 and TaPR1a showed higher accumulations of reactive oxygen species (ROS) during plant defense responses. All these findings suggested that TaLTP3 is involved in wheat resistance against leaf rust pathogen infection and forming a TaLTP3-TaPR1a complex in apoplast against this pathogen, which provides new insights into the functional roles of PR proteins.Jiaojie ZhaoWeishuai BiShuqing ZhaoJun SuMengyu LiLisong MaLisong MaXiumei YuXiaodong WangFrontiers Media S.A.articlewheatpathogenesis-related proteinlipid transfer proteinapoplasticresistancePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wheat pathogenesis-related protein lipid transfer protein apoplastic resistance Plant culture SB1-1110
spellingShingle	wheat pathogenesis-related protein lipid transfer protein apoplastic resistance Plant culture SB1-1110 Jiaojie Zhao Weishuai Bi Shuqing Zhao Jun Su Mengyu Li Lisong Ma Lisong Ma Xiumei Yu Xiaodong Wang Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina
description	Plant apoplast serves as the frontier battlefield of plant defense in response to different types of pathogens. Many pathogenesis-related (PR) proteins are accumulated in apoplastic space during the onset of plant–pathogen interaction, where they act to suppress pathogen infection. In this study, we found the expression of Triticum aestivum lipid transfer protein 3 (TaLTP3) gene was unregulated during incompatible interaction mediated by leaf rust resistance genes Lr39/41 at the early infection stage. Stable transgenic wheat lines overexpressing TaLTP3 exhibited enhanced resistance to leaf rust pathogen Puccinia triticina. Transcriptome analysis revealed that overexpression of TaLTP3 specifically activated the transcription of pathogenesis-related protein 1a (TaPR1a) and multiple plant hormone pathways, including salicylic acid (SA), jasmonic acid (JA), and auxin, in response to the infection of the model bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Further investigation indicated that TaLTP3 physically associated with wheat TaPR1a protein in the apoplast. Transgenic wheat lines overexpressing TaLTP3 and TaPR1a showed higher accumulations of reactive oxygen species (ROS) during plant defense responses. All these findings suggested that TaLTP3 is involved in wheat resistance against leaf rust pathogen infection and forming a TaLTP3-TaPR1a complex in apoplast against this pathogen, which provides new insights into the functional roles of PR proteins.
format	article
author	Jiaojie Zhao Weishuai Bi Shuqing Zhao Jun Su Mengyu Li Lisong Ma Lisong Ma Xiumei Yu Xiaodong Wang
author_facet	Jiaojie Zhao Weishuai Bi Shuqing Zhao Jun Su Mengyu Li Lisong Ma Lisong Ma Xiumei Yu Xiaodong Wang
author_sort	Jiaojie Zhao
title	Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina
title_short	Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina
title_full	Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina
title_fullStr	Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina
title_full_unstemmed	Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina
title_sort	wheat apoplast-localized lipid transfer protein taltp3 enhances defense responses against puccinia triticina
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0330d3f067954c5ba550c8328d51b4ce
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Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina

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