The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia
Abstract Background Vaginal microbiota (VMB) composition is altered in women with cervical intra-epithelial neoplasia (CIN) compared to healthy controls and is associated with disease progression. However, the impact of CIN excision on the VMB and innate immunity is not known. This observational stu...
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2021
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oai:doaj.org-article:033270973974461f994114182683b4cf2021-11-08T10:57:58ZThe vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia10.1186/s13073-021-00977-w1756-994Xhttps://doaj.org/article/033270973974461f994114182683b4cf2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13073-021-00977-whttps://doaj.org/toc/1756-994XAbstract Background Vaginal microbiota (VMB) composition is altered in women with cervical intra-epithelial neoplasia (CIN) compared to healthy controls and is associated with disease progression. However, the impact of CIN excision on the VMB and innate immunity is not known. This observational study aims to explore the impact of CIN excision on the VMB, antimicrobial peptides (AMP) and proinflammatory cytokines. Methods We sampled 103 non-pregnant, premenopausal women at the time of excisional treatment for CIN and at their 6-month follow-up visit. A further 39 untreated controls with normal cytology were also sampled. We used metataxonomics to group vaginal swab samples into community state types (CSTs) and ELISA to quantify cytokine and AMP levels in matched vaginal secretions. Analyses were performed to compare the bacterial composition and immune analyte levels before and after CIN excision and in healthy controls. Results Women with CIN had significantly higher rates of Lactobacillus species depletion pre-treatment compared to healthy controls (CST IV 21/103, 20% vs 1/39, 3%, p = 0.0081). Excision did not change the VMB composition, with CST IV remaining significantly more prevalent after excision compared to untreated, healthy controls (CST IV 19/103, 20% vs 1/39, 3%, p = 0.0142). Prevotella bivia and Sneathia amnii were significantly higher in samples before treatment compared to untreated controls, and Prevotella bivia remained significantly higher amongst the treated, with less Lactobacillus crispatus compared to untreated controls. IL-1β and IL-8 remained significantly elevated pre- (p < 0.0001 and p = 0.0014, respectively) and post-treatment (p < 0.0001 and p = 0.0035, respectively) compared to untreated controls. Levels of human beta-defensin-1 and secretory leukocyte protease inhibitor were both significantly reduced following CIN excision (p < 0.0001); however, their levels remained lower than controls post-treatment. Conclusions Women with CIN have an increased prevalence of Lactobacillus sp. depletion, high-diversity VMB composition, and higher levels of proinflammatory cytokines and AMPs compared to normal controls. Surgical excision of the disease reduces levels of vaginal AMPs but does not alter VMB composition or cytokine levels. These findings suggest that women with CIN have an inherent predisposition to a high-diversity proinflammatory environment that is not corrected by disease excision. The failure to re-establish a Lactobacillus-enriched CST may explain why women remain at high risk of pre-invasive and invasive disease recurrence.Anita MitraDavid A. MacIntyreMaria ParaskevaidiAnna-Barbara MoscickiVishakha MahajanAnn SmithYun S. LeeDeirdre LyonsEvangelos ParaskevaidisJulian R. MarchesiPhillip R. BennettMaria KyrgiouBMCarticleVaginal microbiotaMetataxonomicsMucosal immunityCervical intraepithelial neoplasiaLactobacillusMedicineRGeneticsQH426-470ENGenome Medicine, Vol 13, Iss 1, Pp 1-18 (2021) |
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DOAJ |
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EN |
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Vaginal microbiota Metataxonomics Mucosal immunity Cervical intraepithelial neoplasia Lactobacillus Medicine R Genetics QH426-470 |
| spellingShingle |
Vaginal microbiota Metataxonomics Mucosal immunity Cervical intraepithelial neoplasia Lactobacillus Medicine R Genetics QH426-470 Anita Mitra David A. MacIntyre Maria Paraskevaidi Anna-Barbara Moscicki Vishakha Mahajan Ann Smith Yun S. Lee Deirdre Lyons Evangelos Paraskevaidis Julian R. Marchesi Phillip R. Bennett Maria Kyrgiou The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| description |
Abstract Background Vaginal microbiota (VMB) composition is altered in women with cervical intra-epithelial neoplasia (CIN) compared to healthy controls and is associated with disease progression. However, the impact of CIN excision on the VMB and innate immunity is not known. This observational study aims to explore the impact of CIN excision on the VMB, antimicrobial peptides (AMP) and proinflammatory cytokines. Methods We sampled 103 non-pregnant, premenopausal women at the time of excisional treatment for CIN and at their 6-month follow-up visit. A further 39 untreated controls with normal cytology were also sampled. We used metataxonomics to group vaginal swab samples into community state types (CSTs) and ELISA to quantify cytokine and AMP levels in matched vaginal secretions. Analyses were performed to compare the bacterial composition and immune analyte levels before and after CIN excision and in healthy controls. Results Women with CIN had significantly higher rates of Lactobacillus species depletion pre-treatment compared to healthy controls (CST IV 21/103, 20% vs 1/39, 3%, p = 0.0081). Excision did not change the VMB composition, with CST IV remaining significantly more prevalent after excision compared to untreated, healthy controls (CST IV 19/103, 20% vs 1/39, 3%, p = 0.0142). Prevotella bivia and Sneathia amnii were significantly higher in samples before treatment compared to untreated controls, and Prevotella bivia remained significantly higher amongst the treated, with less Lactobacillus crispatus compared to untreated controls. IL-1β and IL-8 remained significantly elevated pre- (p < 0.0001 and p = 0.0014, respectively) and post-treatment (p < 0.0001 and p = 0.0035, respectively) compared to untreated controls. Levels of human beta-defensin-1 and secretory leukocyte protease inhibitor were both significantly reduced following CIN excision (p < 0.0001); however, their levels remained lower than controls post-treatment. Conclusions Women with CIN have an increased prevalence of Lactobacillus sp. depletion, high-diversity VMB composition, and higher levels of proinflammatory cytokines and AMPs compared to normal controls. Surgical excision of the disease reduces levels of vaginal AMPs but does not alter VMB composition or cytokine levels. These findings suggest that women with CIN have an inherent predisposition to a high-diversity proinflammatory environment that is not corrected by disease excision. The failure to re-establish a Lactobacillus-enriched CST may explain why women remain at high risk of pre-invasive and invasive disease recurrence. |
| format |
article |
| author |
Anita Mitra David A. MacIntyre Maria Paraskevaidi Anna-Barbara Moscicki Vishakha Mahajan Ann Smith Yun S. Lee Deirdre Lyons Evangelos Paraskevaidis Julian R. Marchesi Phillip R. Bennett Maria Kyrgiou |
| author_facet |
Anita Mitra David A. MacIntyre Maria Paraskevaidi Anna-Barbara Moscicki Vishakha Mahajan Ann Smith Yun S. Lee Deirdre Lyons Evangelos Paraskevaidis Julian R. Marchesi Phillip R. Bennett Maria Kyrgiou |
| author_sort |
Anita Mitra |
| title |
The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| title_short |
The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| title_full |
The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| title_fullStr |
The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| title_full_unstemmed |
The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| title_sort |
vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/033270973974461f994114182683b4cf |
| work_keys_str_mv |
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