Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status

Sunarto, Setyaningsih R, Yanti A. 2016. Identification of denitrifying bacteria from sediments of Rawa Jombor waters,Â Central Java and its trophic status. Biodiversitas 17: 578-584. Pooled freshwater (lentic) is susceptible to contamination. One of pollution contributors on freshwater is the agricu...

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Autores principales:	SUNARTO SUNARTO, RATNA SETYANINGSIH, ANDRI YANTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2016
Materias:	denitrifying bacteria, sediment, rawa jombor, nitrate, 16s rrna gene. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/033464f7f18a4c0d8d7189b1881537af
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spelling	oai:doaj.org-article:033464f7f18a4c0d8d7189b1881537af2021-11-14T02:27:09ZIdentification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status1412-033X2085-472210.13057/biodiv/d170218https://doaj.org/article/033464f7f18a4c0d8d7189b1881537af2016-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/18https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Sunarto, Setyaningsih R, Yanti A. 2016. Identification of denitrifying bacteria from sediments of Rawa Jombor waters,Â Central Java and its trophic status. Biodiversitas 17: 578-584. Pooled freshwater (lentic) is susceptible to contamination. One of pollution contributors on freshwater is the agricultural sector. One of them is the inclusion of waste in the form of organic and inorganic materials. The waste will increase the nutrient in waters causing sedimentation, eutrophication and pollution. The pollution is from nitrates derived from agricultural wastes using inorganic fertilizers. An alternative solution for the prevention and tackling of it is to utilize aquatic microorganisms namely denitrifying bacteria. These bacteria can convert nitrates into nitrogen gas (N2) in the anaerobic state so that it can handle the pollution of nitrate in water. This study aimsed to identify the denitrifying bacteria isolated from aquatic sediments of Rawa Jombor and to determine trophic status of Rawa Jombor waters. Identification of denitrifying bacteria was doneÂ through the morphology and physiology testing phase and also molecular analysis of 16S rRNA gene sequence. Based on parameters of total nitrogen, phosphor and brightness referring to the criteria of the lake trophic status attached to Ministry of Environment (PerMNLH)Â No. 28 2009, Rawa Jombor aquatic trophic status was analyzed. The results showed that as many as 6 denitrifying bacteria were isolated from sediments in Rawa Jombor waters, Klaten. Characteristics of bacteria colonies were translucent, round in shape, Gram-negative, rod and motile shaped cells. Based on the analysis of 16S rRNA gene sequence, all of denitrifying bacteria isolates were identified as having the highest similarity to the Shewanella genus. TmD isolate was identified as Shewanella putrefaciens, while TmE, TmG and TmI isolates were identified as Shewanella genus and TmA isolates was new species. Trophic status of Rawa Jombor aquatic was hypereutrophication.SUNARTO SUNARTORATNA SETYANINGSIHANDRI YANTIMBI & UNS Soloarticledenitrifying bacteria, sediment, rawa jombor, nitrate, 16s rrna gene.Biology (General)QH301-705.5ENBiodiversitas, Vol 17, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	denitrifying bacteria, sediment, rawa jombor, nitrate, 16s rrna gene. Biology (General) QH301-705.5
spellingShingle	denitrifying bacteria, sediment, rawa jombor, nitrate, 16s rrna gene. Biology (General) QH301-705.5 SUNARTO SUNARTO RATNA SETYANINGSIH ANDRI YANTI Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status
description	Sunarto, Setyaningsih R, Yanti A. 2016. Identification of denitrifying bacteria from sediments of Rawa Jombor waters,Â Central Java and its trophic status. Biodiversitas 17: 578-584. Pooled freshwater (lentic) is susceptible to contamination. One of pollution contributors on freshwater is the agricultural sector. One of them is the inclusion of waste in the form of organic and inorganic materials. The waste will increase the nutrient in waters causing sedimentation, eutrophication and pollution. The pollution is from nitrates derived from agricultural wastes using inorganic fertilizers. An alternative solution for the prevention and tackling of it is to utilize aquatic microorganisms namely denitrifying bacteria. These bacteria can convert nitrates into nitrogen gas (N2) in the anaerobic state so that it can handle the pollution of nitrate in water. This study aimsed to identify the denitrifying bacteria isolated from aquatic sediments of Rawa Jombor and to determine trophic status of Rawa Jombor waters. Identification of denitrifying bacteria was doneÂ through the morphology and physiology testing phase and also molecular analysis of 16S rRNA gene sequence. Based on parameters of total nitrogen, phosphor and brightness referring to the criteria of the lake trophic status attached to Ministry of Environment (PerMNLH)Â No. 28 2009, Rawa Jombor aquatic trophic status was analyzed. The results showed that as many as 6 denitrifying bacteria were isolated from sediments in Rawa Jombor waters, Klaten. Characteristics of bacteria colonies were translucent, round in shape, Gram-negative, rod and motile shaped cells. Based on the analysis of 16S rRNA gene sequence, all of denitrifying bacteria isolates were identified as having the highest similarity to the Shewanella genus. TmD isolate was identified as Shewanella putrefaciens, while TmE, TmG and TmI isolates were identified as Shewanella genus and TmA isolates was new species. Trophic status of Rawa Jombor aquatic was hypereutrophication.
format	article
author	SUNARTO SUNARTO RATNA SETYANINGSIH ANDRI YANTI
author_facet	SUNARTO SUNARTO RATNA SETYANINGSIH ANDRI YANTI
author_sort	SUNARTO SUNARTO
title	Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status
title_short	Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status
title_full	Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status
title_fullStr	Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status
title_full_unstemmed	Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status
title_sort	identification of denitrifying bacteria from sediments of rawa jombor waters, central java and its trophic status
publisher	MBI & UNS Solo
publishDate	2016
url	https://doaj.org/article/033464f7f18a4c0d8d7189b1881537af
work_keys_str_mv	AT sunartosunarto identificationofdenitrifyingbacteriafromsedimentsofrawajomborwaterscentraljavaanditstrophicstatus AT ratnasetyaningsih identificationofdenitrifyingbacteriafromsedimentsofrawajomborwaterscentraljavaanditstrophicstatus AT andriyanti identificationofdenitrifyingbacteriafromsedimentsofrawajomborwaterscentraljavaanditstrophicstatus
_version_	1718430056413396992

Identification of denitrifying bacteria from sediments of Rawa Jombor waters, Central Java and its trophic status

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