Three-Dimensional Visualization of APEX2-Tagged Erg11 in <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Using Focused Ion Beam Scanning Electron Microscopy

Three-Dimensional Visualization of APEX2-Tagged Erg11 in <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Using Focused Ion Beam Scanning Electron Microscopy

ABSTRACT The determination of the exact location of a protein in the cell is essential to the understanding of biological processes. Here, we report for the first time the visualization of a protein of interest in Saccharomyces cerevisiae using focused ion beam scanning electron microscopy (FIB-SEM)...

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Autores principales: Winnie Kerstens, Anneke Kremer, Michelle Holtappels, Peter Borghgraef, Saskia Lippens, Patrick Van Dijck
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2020
Materias:
FIB-SEM
electron microscopy
Saccharomyces cerevisiae
yeast
APEX2
DAB
Microbiology
QR1-502
Acceso en línea:https://doaj.org/article/03364b78ae7b4984a449b07f752521ff
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