Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis

Abstract Eotaxin-1 (CCL11) induces the migration of different leukocyte types by interacting with CCR3. In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLS) are pathogenic effectors and a major CCR3-expressing cell. The aim of this study was to investigate the expression and function of...

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Autores principales:	Kuninobu Wakabayashi, Takeo Isozaki, Yumi Tsubokura, Sayaka Fukuse, Tsuyoshi Kasama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/034ee81ee5084fe59f2a54cce030bd4a
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spelling	oai:doaj.org-article:034ee81ee5084fe59f2a54cce030bd4a2021-12-02T15:51:12ZEotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis10.1038/s41598-021-87199-72045-2322https://doaj.org/article/034ee81ee5084fe59f2a54cce030bd4a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87199-7https://doaj.org/toc/2045-2322Abstract Eotaxin-1 (CCL11) induces the migration of different leukocyte types by interacting with CCR3. In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLS) are pathogenic effectors and a major CCR3-expressing cell. The aim of this study was to investigate the expression and function of CCL11 in RA FLS. The expression of CCL11 and CCR3 was evaluated by ELISA, immunofluorescence and quantitative PCR analysis. The CCL11 levels in serum and synovial fluids (SFs) from RA patients were significantly higher than those in serum from healthy controls and SFs from osteoarthritis patients. CCL11 and CCR3 were expressed in the RA synovial tissue lining layers. The secretion of CCL11 in RA FLS-conditioned medium and the mRNA expression of CCL11 and CCR3 were induced by TNF-α. Furthermore, CCL11 induced the mRNA expression of CCL11 and CCR3. Application of a CCR3 antagonist reduced TNF-α-induced CCL11 secretion from RA FLS. CCL11 induced the migration of RA FLS and monocytes. RA FLS migration was decreased by treatment with CCL11 siRNA. The migration of monocytes to medium conditioned with CCL11 siRNA-transfected and TNF-α-stimulated RA FLS was reduced. These data indicate that the self-amplification of CCL11 via CCR3 may play an important role in cell migration in RA.Kuninobu WakabayashiTakeo IsozakiYumi TsubokuraSayaka FukuseTsuyoshi KasamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kuninobu Wakabayashi Takeo Isozaki Yumi Tsubokura Sayaka Fukuse Tsuyoshi Kasama Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis
description	Abstract Eotaxin-1 (CCL11) induces the migration of different leukocyte types by interacting with CCR3. In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLS) are pathogenic effectors and a major CCR3-expressing cell. The aim of this study was to investigate the expression and function of CCL11 in RA FLS. The expression of CCL11 and CCR3 was evaluated by ELISA, immunofluorescence and quantitative PCR analysis. The CCL11 levels in serum and synovial fluids (SFs) from RA patients were significantly higher than those in serum from healthy controls and SFs from osteoarthritis patients. CCL11 and CCR3 were expressed in the RA synovial tissue lining layers. The secretion of CCL11 in RA FLS-conditioned medium and the mRNA expression of CCL11 and CCR3 were induced by TNF-α. Furthermore, CCL11 induced the mRNA expression of CCL11 and CCR3. Application of a CCR3 antagonist reduced TNF-α-induced CCL11 secretion from RA FLS. CCL11 induced the migration of RA FLS and monocytes. RA FLS migration was decreased by treatment with CCL11 siRNA. The migration of monocytes to medium conditioned with CCL11 siRNA-transfected and TNF-α-stimulated RA FLS was reduced. These data indicate that the self-amplification of CCL11 via CCR3 may play an important role in cell migration in RA.
format	article
author	Kuninobu Wakabayashi Takeo Isozaki Yumi Tsubokura Sayaka Fukuse Tsuyoshi Kasama
author_facet	Kuninobu Wakabayashi Takeo Isozaki Yumi Tsubokura Sayaka Fukuse Tsuyoshi Kasama
author_sort	Kuninobu Wakabayashi
title	Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis
title_short	Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis
title_full	Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis
title_fullStr	Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis
title_full_unstemmed	Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis
title_sort	eotaxin-1/ccl11 is involved in cell migration in rheumatoid arthritis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/034ee81ee5084fe59f2a54cce030bd4a
work_keys_str_mv	AT kuninobuwakabayashi eotaxin1ccl11isinvolvedincellmigrationinrheumatoidarthritis AT takeoisozaki eotaxin1ccl11isinvolvedincellmigrationinrheumatoidarthritis AT yumitsubokura eotaxin1ccl11isinvolvedincellmigrationinrheumatoidarthritis AT sayakafukuse eotaxin1ccl11isinvolvedincellmigrationinrheumatoidarthritis AT tsuyoshikasama eotaxin1ccl11isinvolvedincellmigrationinrheumatoidarthritis
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Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis

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