Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin

In this study, a laccase-mediator system (LMS) using a natural mediator was developed as a whitening agent for melanin decolorization. Seven natural mediators were used to replace synthetic mediators and successfully overcome the low redox potential of laccase and limited access of melanin to the ac...

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Autores principales:	Saerom Park, Dahun Jung, Hyejin Do, Jonghyeon Yun, Dongjun Lee, Soeun Hwang, Sang Hyun Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	laccase melanin decolorization natural mediators Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/0355e592b1f04c7e8343b607b36bccfd
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spelling	oai:doaj.org-article:0355e592b1f04c7e8343b607b36bccfd2021-11-11T18:43:22ZLaccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin10.3390/polym132136712073-4360https://doaj.org/article/0355e592b1f04c7e8343b607b36bccfd2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3671https://doaj.org/toc/2073-4360In this study, a laccase-mediator system (LMS) using a natural mediator was developed as a whitening agent for melanin decolorization. Seven natural mediators were used to replace synthetic mediators and successfully overcome the low redox potential of laccase and limited access of melanin to the active site of laccase. The melanin decolorization activity of laccases from <i>Trametes versicolor</i> (lacT) and <i>Myceliophthora thermophila</i> (lacM) was significantly enhanced using natural mediators including acetosyringone, syringaldehyde, and acetovanillone, which showed low cytotoxicity. The methoxy and ketone groups of natural mediators play an important role in melanin decolorization. The specificity constants of lacT and lacM for melanin decolorization were enhanced by 247 and 334, respectively, when acetosyringone was used as a mediator. LMS using lacM and acetosyringone could also decolorize the melanin present in the cellulose hydrogel film, which mimics the skin condition. Furthermore, LMS could decolorize not only synthetic eumelanin analogs prepared by the oxidation of tyrosine but also natural melanin produced by melanoma cells.Saerom ParkDahun JungHyejin DoJonghyeon YunDongjun LeeSoeun HwangSang Hyun LeeMDPI AGarticlelaccasemelanindecolorizationnatural mediatorsOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3671, p 3671 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laccase melanin decolorization natural mediators Organic chemistry QD241-441
spellingShingle	laccase melanin decolorization natural mediators Organic chemistry QD241-441 Saerom Park Dahun Jung Hyejin Do Jonghyeon Yun Dongjun Lee Soeun Hwang Sang Hyun Lee Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin
description	In this study, a laccase-mediator system (LMS) using a natural mediator was developed as a whitening agent for melanin decolorization. Seven natural mediators were used to replace synthetic mediators and successfully overcome the low redox potential of laccase and limited access of melanin to the active site of laccase. The melanin decolorization activity of laccases from <i>Trametes versicolor</i> (lacT) and <i>Myceliophthora thermophila</i> (lacM) was significantly enhanced using natural mediators including acetosyringone, syringaldehyde, and acetovanillone, which showed low cytotoxicity. The methoxy and ketone groups of natural mediators play an important role in melanin decolorization. The specificity constants of lacT and lacM for melanin decolorization were enhanced by 247 and 334, respectively, when acetosyringone was used as a mediator. LMS using lacM and acetosyringone could also decolorize the melanin present in the cellulose hydrogel film, which mimics the skin condition. Furthermore, LMS could decolorize not only synthetic eumelanin analogs prepared by the oxidation of tyrosine but also natural melanin produced by melanoma cells.
format	article
author	Saerom Park Dahun Jung Hyejin Do Jonghyeon Yun Dongjun Lee Soeun Hwang Sang Hyun Lee
author_facet	Saerom Park Dahun Jung Hyejin Do Jonghyeon Yun Dongjun Lee Soeun Hwang Sang Hyun Lee
author_sort	Saerom Park
title	Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin
title_short	Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin
title_full	Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin
title_fullStr	Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin
title_full_unstemmed	Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin
title_sort	laccase-mediator system using a natural mediator as a whitening agent for the decolorization of melanin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0355e592b1f04c7e8343b607b36bccfd
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Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin

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