Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)

Moallem E, Ghasemipirbalouti A, Nejadsattari T, Iranbakhsh A, Mehregan I. 2017. Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae). Biodiversitas 18: 1430-1437. Physalis divaricata (Solana...

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Autores principales:	ELHAM MOALLEM, ABDOLLAH GHASEMIPIRBALOUTI, TAHER NEJADSATTARI, ALIREZA IRANBAKHSH, IRAJ MEHREGAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	histochemical physalis divaricata pmt rt-qpcr wagner test Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/03572c66df064a8ebd8770349601f48c
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spelling	oai:doaj.org-article:03572c66df064a8ebd8770349601f48c2021-11-16T14:00:12ZEvaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)1412-033X2085-472210.13057/biodiv/d180418https://doaj.org/article/03572c66df064a8ebd8770349601f48c2017-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2095https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Moallem E, Ghasemipirbalouti A, Nejadsattari T, Iranbakhsh A, Mehregan I. 2017. Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae). Biodiversitas 18: 1430-1437. Physalis divaricata (Solanaceae) is one of the most prevalent weeds in summer crops. Putrescine Nmethyltransferase (PMT) is a key enzyme in the biosynthesis of nicotine, tropane alkaloids atropine, scopolamine, cocaine, and calystegines. The present study set to compare PMT gene expression during different growth stages of Ph. divaricata using RT-qPCR assay. RNA extraction was performed from root and leaf samples of a total number of 40 individuals Ph. divaricata at different growth stages (late vegetative and fruiting stages) collected from southwestern Iran. RT-qPCR of cDNA reversely synthesized from RNA was carried out using SYBRÂ®Premix Ex TaqTM II kit. PMT gene expression levels were analyzed using Î”Î”CT method. The results showed that expression level of PMT in late vegetative stage samples was significantly higher compared to fruiting stage samples. The expression level of PMT similarly changed in root and leaf samples. Direct visualization of alkaloids in different tissues using Wagner histochemical tests showed more concentrations of alkaloids in leaf idioblasts and root stele.ELHAM MOALLEMABDOLLAH GHASEMIPIRBALOUTITAHER NEJADSATTARIALIREZA IRANBAKHSHIRAJ MEHREGANMBI & UNS Soloarticlehistochemicalphysalis divaricatapmtrt-qpcrwagner testBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 4, Pp 1430-1437 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	histochemical physalis divaricata pmt rt-qpcr wagner test Biology (General) QH301-705.5
spellingShingle	histochemical physalis divaricata pmt rt-qpcr wagner test Biology (General) QH301-705.5 ELHAM MOALLEM ABDOLLAH GHASEMIPIRBALOUTI TAHER NEJADSATTARI ALIREZA IRANBAKHSH IRAJ MEHREGAN Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)
description	Moallem E, Ghasemipirbalouti A, Nejadsattari T, Iranbakhsh A, Mehregan I. 2017. Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae). Biodiversitas 18: 1430-1437. Physalis divaricata (Solanaceae) is one of the most prevalent weeds in summer crops. Putrescine Nmethyltransferase (PMT) is a key enzyme in the biosynthesis of nicotine, tropane alkaloids atropine, scopolamine, cocaine, and calystegines. The present study set to compare PMT gene expression during different growth stages of Ph. divaricata using RT-qPCR assay. RNA extraction was performed from root and leaf samples of a total number of 40 individuals Ph. divaricata at different growth stages (late vegetative and fruiting stages) collected from southwestern Iran. RT-qPCR of cDNA reversely synthesized from RNA was carried out using SYBRÂ®Premix Ex TaqTM II kit. PMT gene expression levels were analyzed using Î”Î”CT method. The results showed that expression level of PMT in late vegetative stage samples was significantly higher compared to fruiting stage samples. The expression level of PMT similarly changed in root and leaf samples. Direct visualization of alkaloids in different tissues using Wagner histochemical tests showed more concentrations of alkaloids in leaf idioblasts and root stele.
format	article
author	ELHAM MOALLEM ABDOLLAH GHASEMIPIRBALOUTI TAHER NEJADSATTARI ALIREZA IRANBAKHSH IRAJ MEHREGAN
author_facet	ELHAM MOALLEM ABDOLLAH GHASEMIPIRBALOUTI TAHER NEJADSATTARI ALIREZA IRANBAKHSH IRAJ MEHREGAN
author_sort	ELHAM MOALLEM
title	Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)
title_short	Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)
title_full	Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)
title_fullStr	Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)
title_full_unstemmed	Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)
title_sort	evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant physalis divaricata (solanaceae)
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/03572c66df064a8ebd8770349601f48c
work_keys_str_mv	AT elhammoallem evaluationofexpressionanalysisofputrescinenmethyltransferasegeneduringdifferentstagesofgrowthinthemedicinalplantphysalisdivaricatasolanaceae AT abdollahghasemipirbalouti evaluationofexpressionanalysisofputrescinenmethyltransferasegeneduringdifferentstagesofgrowthinthemedicinalplantphysalisdivaricatasolanaceae AT tahernejadsattari evaluationofexpressionanalysisofputrescinenmethyltransferasegeneduringdifferentstagesofgrowthinthemedicinalplantphysalisdivaricatasolanaceae AT alirezairanbakhsh evaluationofexpressionanalysisofputrescinenmethyltransferasegeneduringdifferentstagesofgrowthinthemedicinalplantphysalisdivaricatasolanaceae AT irajmehregan evaluationofexpressionanalysisofputrescinenmethyltransferasegeneduringdifferentstagesofgrowthinthemedicinalplantphysalisdivaricatasolanaceae
_version_	1718426460744581120

Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)

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