Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt

Rift Valley Fever (RVF) is an endemic disease in Egypt causing disease in animals and humans since the 1977. Full molecular description of the national vaccine strain is a strategic concern to ensure the antigenic makeup of local vaccine is adequate to provide the required protective immunity for th...

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Autores principales:	Abo Hatab E. M., M. H. Ali, Atwa, M. H., Abul Magd, D.M., Ahmed F. Soudy, Saad A. Moussa
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2019
Materias:	classical bursa elisa ibd inactivated vaccine isolates of snt Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2019.62672 https://doaj.org/article/0359600e69874f72a6bf69ba56849edf
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spelling	oai:doaj.org-article:0359600e69874f72a6bf69ba56849edf2021-12-02T13:21:46ZPropagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptthttps://dx.doi.org/10.21608/javs.2019.626721687-40722090-3308https://doaj.org/article/0359600e69874f72a6bf69ba56849edf2019-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62672.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Rift Valley Fever (RVF) is an endemic disease in Egypt causing disease in animals and humans since the 1977. Full molecular description of the national vaccine strain is a strategic concern to ensure the antigenic makeup of local vaccine is adequate to provide the required protective immunity for the vaccinated animal species and as a tool of final product quality control. Molecular characterization and phylogenetic analysis of Gc gene of M segment of RVF-ZH501 strain which used in local vaccine production in veterinary serum and vaccine research institute (VSVRI) were carried out in this study. RVF-ZH501 strain was propagated by passages on BHK cell line and then purified my plaque purification technique. RT-PCR for amplification of the Gc gene of M segment. Sequence analysis of the obtained PCR product carried out by MEGA7 program. The result of the study revealed that RVF strain ZH501 Gc genomic content has 99.5 % molecular identity to the strains firstly isolated in Egypt in 1977, so still suitable for vaccine production. Abo Hatab E. M.M. H. AliAtwa, M. H.Abul Magd, D.M.Ahmed F. SoudySaad A. MoussaEgyptian Society for Animal Managementarticleclassical bursaelisaibdinactivated vaccineisolates of sntZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 4, Iss 1, Pp 13-17 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	classical bursa elisa ibd inactivated vaccine isolates of snt Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	classical bursa elisa ibd inactivated vaccine isolates of snt Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Abo Hatab E. M. M. H. Ali Atwa, M. H. Abul Magd, D.M. Ahmed F. Soudy Saad A. Moussa Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt
description	Rift Valley Fever (RVF) is an endemic disease in Egypt causing disease in animals and humans since the 1977. Full molecular description of the national vaccine strain is a strategic concern to ensure the antigenic makeup of local vaccine is adequate to provide the required protective immunity for the vaccinated animal species and as a tool of final product quality control. Molecular characterization and phylogenetic analysis of Gc gene of M segment of RVF-ZH501 strain which used in local vaccine production in veterinary serum and vaccine research institute (VSVRI) were carried out in this study. RVF-ZH501 strain was propagated by passages on BHK cell line and then purified my plaque purification technique. RT-PCR for amplification of the Gc gene of M segment. Sequence analysis of the obtained PCR product carried out by MEGA7 program. The result of the study revealed that RVF strain ZH501 Gc genomic content has 99.5 % molecular identity to the strains firstly isolated in Egypt in 1977, so still suitable for vaccine production.
format	article
author	Abo Hatab E. M. M. H. Ali Atwa, M. H. Abul Magd, D.M. Ahmed F. Soudy Saad A. Moussa
author_facet	Abo Hatab E. M. M. H. Ali Atwa, M. H. Abul Magd, D.M. Ahmed F. Soudy Saad A. Moussa
author_sort	Abo Hatab E. M.
title	Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt
title_short	Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt
title_full	Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt
title_fullStr	Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt
title_full_unstemmed	Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt
title_sort	propagation, purification and molecular characterization of rvf virus (zh 501) strain for vaccine production in egyptt
publisher	Egyptian Society for Animal Management
publishDate	2019
url	https://dx.doi.org/10.21608/javs.2019.62672 https://doaj.org/article/0359600e69874f72a6bf69ba56849edf
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_version_	1718393131200675840

Propagation, Purification and Molecular Characterization of RVF virus (ZH 501) Strain for Vaccine Production in Egyptt

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