Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain

Aitao Wang,1 Dongmei Guo,1 Hongyu Cheng,2 Hui Jiang,3 Xiaojuan Liu,1 Zhizhong Yun4 1Department of Anesthesiology, Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia, 010017, People’s Republic of China; 2Department of Anesthesiology, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 01...

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Autores principales:	Wang A, Guo D, Cheng H, Jiang H, Liu X, Yun Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	breast cancer bone cancer pain baicalin transcriptome sequencing Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0362cbba23e1432ea588742db8183b74
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spelling	oai:doaj.org-article:0362cbba23e1432ea588742db8183b742021-12-02T18:43:43ZTranscriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain1178-7031https://doaj.org/article/0362cbba23e1432ea588742db8183b742021-11-01T00:00:00Zhttps://www.dovepress.com/transcriptome-sequencing-explores-the-mechanism-of-baicalin-on-bone-ca-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Aitao Wang,1 Dongmei Guo,1 Hongyu Cheng,2 Hui Jiang,3 Xiaojuan Liu,1 Zhizhong Yun4 1Department of Anesthesiology, Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia, 010017, People’s Republic of China; 2Department of Anesthesiology, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010110, People’s Republic of China; 3Department of Anesthesiology, Baotou Medical College, Baotou, Inner Mongolia, 014040, People’s Republic of China; 4Department of Urinary Surgery, Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia, 010017, People’s Republic of ChinaCorrespondence: Zhizhong YunDepartment of Urinary Surgery, Inner Mongolia People’s Hospital, #20, Zhaowuda Road, Saihan District, Hohhot, Inner Mongolia, 010017, People’s Republic of ChinaTel +86-18047191483Email yzzyb168@126.comIntroduction: Bone cancer pain is characterized by persistent pain, usually requiring drugs to relieve pain. Baicalin, a flavonoid compound extracted from Scutellaria baicalensis, which has antioxidant and analgesic effects. But, the effect of baicalin on bone cancer pain is unclear. Thus, this study aimed to explore the mechanism of baicalin on SD rats with bone cancer pain.Materials and Methods: The MADB-106 breast cancer cells-induced bone pain model was constructed and carried out baicalin treatment. The therapeutic effect of baicalin on bone cancer pain model was observed by hematoxylin-eosin staining and immunofluorescence staining. We also performed transcriptome sequencing analysis of baicalin in the treatment of bone metastases. Also, RT-qPCR and ELISA were used to detect the expression levels of inflammation factors.Results: After baicalin treatment, osteoclast activation was inhibited and the number of bone trabeculae was increased. Baicalin inhibited the protein expression level of inflammatory factors (IL-1β, IL-6, TNF-α and PGE2) in the bone metastases group. Based on the transcriptome sequencing of the bone metastases group and the baicalin treatment group, baicalin inhibited the expression of ALPP, DUSP1, CYR61, ALPPL2, SPP1 and TLR4. RT-qPCR was also used to validate the expression levels of these cytokine genes.Conclusion: Baicalin had a certain inhibitory effect on the SD rat model of bone metastasis cancer. These insights can guide future research on the molecular mechanism of bone cancer pain and provide a theoretical basis for baicalin in the treatment of bone pain caused by breast cancer in the future.Keywords: breast cancer, bone cancer pain, baicalin, transcriptome sequencingWang AGuo DCheng HJiang HLiu XYun ZDove Medical Pressarticlebreast cancerbone cancer painbaicalintranscriptome sequencingPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5999-6010 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer bone cancer pain baicalin transcriptome sequencing Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	breast cancer bone cancer pain baicalin transcriptome sequencing Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Wang A Guo D Cheng H Jiang H Liu X Yun Z Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain
description	Aitao Wang,1 Dongmei Guo,1 Hongyu Cheng,2 Hui Jiang,3 Xiaojuan Liu,1 Zhizhong Yun4 1Department of Anesthesiology, Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia, 010017, People’s Republic of China; 2Department of Anesthesiology, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010110, People’s Republic of China; 3Department of Anesthesiology, Baotou Medical College, Baotou, Inner Mongolia, 014040, People’s Republic of China; 4Department of Urinary Surgery, Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia, 010017, People’s Republic of ChinaCorrespondence: Zhizhong YunDepartment of Urinary Surgery, Inner Mongolia People’s Hospital, #20, Zhaowuda Road, Saihan District, Hohhot, Inner Mongolia, 010017, People’s Republic of ChinaTel +86-18047191483Email yzzyb168@126.comIntroduction: Bone cancer pain is characterized by persistent pain, usually requiring drugs to relieve pain. Baicalin, a flavonoid compound extracted from Scutellaria baicalensis, which has antioxidant and analgesic effects. But, the effect of baicalin on bone cancer pain is unclear. Thus, this study aimed to explore the mechanism of baicalin on SD rats with bone cancer pain.Materials and Methods: The MADB-106 breast cancer cells-induced bone pain model was constructed and carried out baicalin treatment. The therapeutic effect of baicalin on bone cancer pain model was observed by hematoxylin-eosin staining and immunofluorescence staining. We also performed transcriptome sequencing analysis of baicalin in the treatment of bone metastases. Also, RT-qPCR and ELISA were used to detect the expression levels of inflammation factors.Results: After baicalin treatment, osteoclast activation was inhibited and the number of bone trabeculae was increased. Baicalin inhibited the protein expression level of inflammatory factors (IL-1β, IL-6, TNF-α and PGE2) in the bone metastases group. Based on the transcriptome sequencing of the bone metastases group and the baicalin treatment group, baicalin inhibited the expression of ALPP, DUSP1, CYR61, ALPPL2, SPP1 and TLR4. RT-qPCR was also used to validate the expression levels of these cytokine genes.Conclusion: Baicalin had a certain inhibitory effect on the SD rat model of bone metastasis cancer. These insights can guide future research on the molecular mechanism of bone cancer pain and provide a theoretical basis for baicalin in the treatment of bone pain caused by breast cancer in the future.Keywords: breast cancer, bone cancer pain, baicalin, transcriptome sequencing
format	article
author	Wang A Guo D Cheng H Jiang H Liu X Yun Z
author_facet	Wang A Guo D Cheng H Jiang H Liu X Yun Z
author_sort	Wang A
title	Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain
title_short	Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain
title_full	Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain
title_fullStr	Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain
title_full_unstemmed	Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain
title_sort	transcriptome sequencing explores the mechanism of baicalin on bone cancer pain
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/0362cbba23e1432ea588742db8183b74
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Transcriptome Sequencing Explores the Mechanism of Baicalin on Bone Cancer Pain

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