Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering

Worldwide, over 20 million patients suffer from bone disorders annually. Bone scaffolds are designed to integrate into host tissue without causing adverse reactions. Recently, chitosan, an easily available natural polymer, has been considered a suitable scaffold for bone tissue growth as it is a bio...

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Autores principales:	Paola Brun, Annj Zamuner, Leonardo Cassari, Gabriella D’Auria, Lucia Falcigno, Stefano Franchi, Giorgio Contini, Martina Marsotto, Chiara Battocchio, Giovanna Iucci, Monica Dettin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chitosan covalent grafting osteoblasts bioactive peptides bone tissue engineering Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/036a04e68d2d4490804f875567c6dcb4
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spelling	oai:doaj.org-article:036a04e68d2d4490804f875567c6dcb42021-11-25T18:29:47ZChitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering10.3390/nano111127842079-4991https://doaj.org/article/036a04e68d2d4490804f875567c6dcb42021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2784https://doaj.org/toc/2079-4991Worldwide, over 20 million patients suffer from bone disorders annually. Bone scaffolds are designed to integrate into host tissue without causing adverse reactions. Recently, chitosan, an easily available natural polymer, has been considered a suitable scaffold for bone tissue growth as it is a biocompatible, biodegradable, and non-toxic material with antimicrobial activity and osteoinductive capacity. In this work, chitosan was covalently and selectively biofunctionalized with two suitably designed bioactive synthetic peptides: a Vitronectin sequence (HVP) and a BMP-2 peptide (GBMP1a). Nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) investigations highlighted the presence of the peptides grafted to chitosan (named Chit-HVP and Chit-GBMP1a). Chit-HVP and Chit-GBMP1a porous scaffolds promoted human osteoblasts adhesion, proliferation, calcium deposition, and gene expression of three crucial osteoblast proteins. In particular, Chit-HVP highly promoted adhesion and proliferation of osteoblasts, while Chit-GBMP1a guided cell differentiation towards osteoblastic phenotype.Paola BrunAnnj ZamunerLeonardo CassariGabriella D’AuriaLucia FalcignoStefano FranchiGiorgio ContiniMartina MarsottoChiara BattocchioGiovanna IucciMonica DettinMDPI AGarticlechitosancovalent graftingosteoblastsbioactive peptidesbone tissue engineeringChemistryQD1-999ENNanomaterials, Vol 11, Iss 2784, p 2784 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chitosan covalent grafting osteoblasts bioactive peptides bone tissue engineering Chemistry QD1-999
spellingShingle	chitosan covalent grafting osteoblasts bioactive peptides bone tissue engineering Chemistry QD1-999 Paola Brun Annj Zamuner Leonardo Cassari Gabriella D’Auria Lucia Falcigno Stefano Franchi Giorgio Contini Martina Marsotto Chiara Battocchio Giovanna Iucci Monica Dettin Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering
description	Worldwide, over 20 million patients suffer from bone disorders annually. Bone scaffolds are designed to integrate into host tissue without causing adverse reactions. Recently, chitosan, an easily available natural polymer, has been considered a suitable scaffold for bone tissue growth as it is a biocompatible, biodegradable, and non-toxic material with antimicrobial activity and osteoinductive capacity. In this work, chitosan was covalently and selectively biofunctionalized with two suitably designed bioactive synthetic peptides: a Vitronectin sequence (HVP) and a BMP-2 peptide (GBMP1a). Nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) investigations highlighted the presence of the peptides grafted to chitosan (named Chit-HVP and Chit-GBMP1a). Chit-HVP and Chit-GBMP1a porous scaffolds promoted human osteoblasts adhesion, proliferation, calcium deposition, and gene expression of three crucial osteoblast proteins. In particular, Chit-HVP highly promoted adhesion and proliferation of osteoblasts, while Chit-GBMP1a guided cell differentiation towards osteoblastic phenotype.
format	article
author	Paola Brun Annj Zamuner Leonardo Cassari Gabriella D’Auria Lucia Falcigno Stefano Franchi Giorgio Contini Martina Marsotto Chiara Battocchio Giovanna Iucci Monica Dettin
author_facet	Paola Brun Annj Zamuner Leonardo Cassari Gabriella D’Auria Lucia Falcigno Stefano Franchi Giorgio Contini Martina Marsotto Chiara Battocchio Giovanna Iucci Monica Dettin
author_sort	Paola Brun
title	Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering
title_short	Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering
title_full	Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering
title_fullStr	Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering
title_full_unstemmed	Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering
title_sort	chitosan covalently functionalized with peptides mapped on vitronectin and bmp-2 for bone tissue engineering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/036a04e68d2d4490804f875567c6dcb4
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Chitosan Covalently Functionalized with Peptides Mapped on Vitronectin and BMP-2 for Bone Tissue Engineering

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