Chemical linkage to injected tissues is a distinctive property of oxidized avidin.

We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups. We also showed, in a mouse pre-clinical model, the usefulness of AvidinOX fo...

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Autores principales:	Rita De Santis, Anna Maria Anastasi, Angela Pelliccia, Antonio Rosi, Claudio Albertoni, Antonio Verdoliva, Fiorella Petronzelli, Valeria D'Alessio, Serenella Serani, Carlo Antonio Nuzzolo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/036fa13eb6ac4e649f88fc98391bfef0
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spelling	oai:doaj.org-article:036fa13eb6ac4e649f88fc98391bfef02021-11-18T06:51:45ZChemical linkage to injected tissues is a distinctive property of oxidized avidin.1932-620310.1371/journal.pone.0021075https://doaj.org/article/036fa13eb6ac4e649f88fc98391bfef02011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21701578/?tool=EBIhttps://doaj.org/toc/1932-6203We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups. We also showed, in a mouse pre-clinical model, the usefulness of AvidinOX for the delivery of radiolabeled biotin to inoperable tumors. Taking into account that AvidinOX is the first oxidized glycoprotein known to chemically link to injected tissues, we tested in the mouse a panel of additional oxidized glycoproteins, with the aim of investigating the phenomenon. We produced oxidized ovalbumin and mannosylated streptavidin which share with avidin glycosylation pattern and tetrameric structure, respectively and found that neither of them linked significantly to cells in vitro nor to injected tissues in vivo, despite the presence of functional aldehyde groups. The study, extended to additional oxidized glycoproteins, showed that the in vivo chemical conjugation is a distinctive property of the oxidized avidin. Relevance of the high cationic charge of avidin into the stable linkage of AvidinOX to tissues is demonstrated as the oxidized acetylated avidin lost the property. Plasmon resonance on matrix proteins and cellular impedance analyses showed in vitro that avidin exhibits a peculiar interaction with proteins and cells that allows the formation of highly stable Schiff's bases, after oxidation.Rita De SantisAnna Maria AnastasiAngela PellicciaAntonio RosiClaudio AlbertoniAntonio VerdolivaFiorella PetronzelliValeria D'AlessioSerenella SeraniCarlo Antonio NuzzoloPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21075 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rita De Santis Anna Maria Anastasi Angela Pelliccia Antonio Rosi Claudio Albertoni Antonio Verdoliva Fiorella Petronzelli Valeria D'Alessio Serenella Serani Carlo Antonio Nuzzolo Chemical linkage to injected tissues is a distinctive property of oxidized avidin.
description	We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups. We also showed, in a mouse pre-clinical model, the usefulness of AvidinOX for the delivery of radiolabeled biotin to inoperable tumors. Taking into account that AvidinOX is the first oxidized glycoprotein known to chemically link to injected tissues, we tested in the mouse a panel of additional oxidized glycoproteins, with the aim of investigating the phenomenon. We produced oxidized ovalbumin and mannosylated streptavidin which share with avidin glycosylation pattern and tetrameric structure, respectively and found that neither of them linked significantly to cells in vitro nor to injected tissues in vivo, despite the presence of functional aldehyde groups. The study, extended to additional oxidized glycoproteins, showed that the in vivo chemical conjugation is a distinctive property of the oxidized avidin. Relevance of the high cationic charge of avidin into the stable linkage of AvidinOX to tissues is demonstrated as the oxidized acetylated avidin lost the property. Plasmon resonance on matrix proteins and cellular impedance analyses showed in vitro that avidin exhibits a peculiar interaction with proteins and cells that allows the formation of highly stable Schiff's bases, after oxidation.
format	article
author	Rita De Santis Anna Maria Anastasi Angela Pelliccia Antonio Rosi Claudio Albertoni Antonio Verdoliva Fiorella Petronzelli Valeria D'Alessio Serenella Serani Carlo Antonio Nuzzolo
author_facet	Rita De Santis Anna Maria Anastasi Angela Pelliccia Antonio Rosi Claudio Albertoni Antonio Verdoliva Fiorella Petronzelli Valeria D'Alessio Serenella Serani Carlo Antonio Nuzzolo
author_sort	Rita De Santis
title	Chemical linkage to injected tissues is a distinctive property of oxidized avidin.
title_short	Chemical linkage to injected tissues is a distinctive property of oxidized avidin.
title_full	Chemical linkage to injected tissues is a distinctive property of oxidized avidin.
title_fullStr	Chemical linkage to injected tissues is a distinctive property of oxidized avidin.
title_full_unstemmed	Chemical linkage to injected tissues is a distinctive property of oxidized avidin.
title_sort	chemical linkage to injected tissues is a distinctive property of oxidized avidin.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/036fa13eb6ac4e649f88fc98391bfef0
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Chemical linkage to injected tissues is a distinctive property of oxidized avidin.

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