EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES

Abstract. A combination of fluorescent staining with Hoechst 33342 dye, and flow cytometry of murine bone marrow cells may be used for separation of a side population (SP), which is highly enriched for hematopoietic stem cells capable of long-term hematopoietic reconstitution in lethally irradiated...

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Autores principales:	O. R. Tsinkalovsky, V. A. Zaidenov, A. S. Pakhotnykh, V. A. Andrianov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	stem cells side population flow cytometry hematopoiesis malignant tissue Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/03792dfd0e7a41ccab6f417ebdc1c8df
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spelling	oai:doaj.org-article:03792dfd0e7a41ccab6f417ebdc1c8df2021-11-18T08:03:38ZEMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES1563-06252313-741X10.15789/1563-0625-2008-4-5-319-326https://doaj.org/article/03792dfd0e7a41ccab6f417ebdc1c8df2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/179https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. A combination of fluorescent staining with Hoechst 33342 dye, and flow cytometry of murine bone marrow cells may be used for separation of a side population (SP), which is highly enriched for hematopoietic stem cells capable of long-term hematopoietic reconstitution in lethally irradiated recipients. Recently, this approach was also applied to analysis of SP cells in several types of non-hematopoietic tissues, and malignant tumours. In spite of yet poor definition of phenotype and functional potency of SP cells from various tissues, the method of SP isolation may be a useful tool for analysis and pre-enrichment of stem cell-like cells of different origin. Present review article presents a brief description of Hoechst 33342-staining approach, and of recent reports concerning SP studies in various normal and malignant tissues. (Med. Immunol., vol. 10, N 4-5, pp 319-326).O. R. TsinkalovskyV. A. ZaidenovA. S. PakhotnykhV. A. AndrianovSPb RAACIarticlestem cellsside populationflow cytometryhematopoiesismalignant tissueImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 4-5, Pp 319-326 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	stem cells side population flow cytometry hematopoiesis malignant tissue Immunologic diseases. Allergy RC581-607
spellingShingle	stem cells side population flow cytometry hematopoiesis malignant tissue Immunologic diseases. Allergy RC581-607 O. R. Tsinkalovsky V. A. Zaidenov A. S. Pakhotnykh V. A. Andrianov EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES
description	Abstract. A combination of fluorescent staining with Hoechst 33342 dye, and flow cytometry of murine bone marrow cells may be used for separation of a side population (SP), which is highly enriched for hematopoietic stem cells capable of long-term hematopoietic reconstitution in lethally irradiated recipients. Recently, this approach was also applied to analysis of SP cells in several types of non-hematopoietic tissues, and malignant tumours. In spite of yet poor definition of phenotype and functional potency of SP cells from various tissues, the method of SP isolation may be a useful tool for analysis and pre-enrichment of stem cell-like cells of different origin. Present review article presents a brief description of Hoechst 33342-staining approach, and of recent reports concerning SP studies in various normal and malignant tissues. (Med. Immunol., vol. 10, N 4-5, pp 319-326).
format	article
author	O. R. Tsinkalovsky V. A. Zaidenov A. S. Pakhotnykh V. A. Andrianov
author_facet	O. R. Tsinkalovsky V. A. Zaidenov A. S. Pakhotnykh V. A. Andrianov
author_sort	O. R. Tsinkalovsky
title	EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES
title_short	EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES
title_full	EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES
title_fullStr	EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES
title_full_unstemmed	EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES
title_sort	employment of a «side population» approach to stem cell isolation in normal and tumor tissues
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/03792dfd0e7a41ccab6f417ebdc1c8df
work_keys_str_mv	AT ortsinkalovsky employmentofasidepopulationapproachtostemcellisolationinnormalandtumortissues AT vazaidenov employmentofasidepopulationapproachtostemcellisolationinnormalandtumortissues AT aspakhotnykh employmentofasidepopulationapproachtostemcellisolationinnormalandtumortissues AT vaandrianov employmentofasidepopulationapproachtostemcellisolationinnormalandtumortissues
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EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES

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