Corneal wound healing is compromised by immunoproteasome deficiency.

Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by...

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Autores principales:	Deborah A Ferrington, Heidi Roehrich, Angela A Chang, Craig W Huang, Marcela Maldonado, Wendy Bratten, Abrar A Rageh, Neal D Heuss, Dale S Gregerson, Elizabeth F Nelson, Ching Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/037c6e22685d48b79d733ac595a54f20
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spelling	oai:doaj.org-article:037c6e22685d48b79d733ac595a54f202021-11-18T08:00:03ZCorneal wound healing is compromised by immunoproteasome deficiency.1932-620310.1371/journal.pone.0054347https://doaj.org/article/037c6e22685d48b79d733ac595a54f202013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23365662/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by comparing corneas from wildtype (WT) mice and those deficient in two immunoproteasome catalytic subunits (lmp7(-/-)/mecl-1(-/-), L7M1). Immunoproteasome expression was confirmed in WT epithelial cells and in cells of the immune system that were present in the cornea. More apoptotic cells were found in both corneal explant cultures and uninjured corneas of L7M1 compared to WT mice. Following mechanical debridement, L7M1 corneas displayed delayed wound healing, including delayed re-epithelialization and re-establishment of the epithelial barrier, as well as altered inflammatory cytokine production compared to WT mice. These results suggest that immunoproteasome plays an important role in corneal homeostasis and wound healing.Deborah A FerringtonHeidi RoehrichAngela A ChangCraig W HuangMarcela MaldonadoWendy BrattenAbrar A RagehNeal D HeussDale S GregersonElizabeth F NelsonChing YuanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54347 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deborah A Ferrington Heidi Roehrich Angela A Chang Craig W Huang Marcela Maldonado Wendy Bratten Abrar A Rageh Neal D Heuss Dale S Gregerson Elizabeth F Nelson Ching Yuan Corneal wound healing is compromised by immunoproteasome deficiency.
description	Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by comparing corneas from wildtype (WT) mice and those deficient in two immunoproteasome catalytic subunits (lmp7(-/-)/mecl-1(-/-), L7M1). Immunoproteasome expression was confirmed in WT epithelial cells and in cells of the immune system that were present in the cornea. More apoptotic cells were found in both corneal explant cultures and uninjured corneas of L7M1 compared to WT mice. Following mechanical debridement, L7M1 corneas displayed delayed wound healing, including delayed re-epithelialization and re-establishment of the epithelial barrier, as well as altered inflammatory cytokine production compared to WT mice. These results suggest that immunoproteasome plays an important role in corneal homeostasis and wound healing.
format	article
author	Deborah A Ferrington Heidi Roehrich Angela A Chang Craig W Huang Marcela Maldonado Wendy Bratten Abrar A Rageh Neal D Heuss Dale S Gregerson Elizabeth F Nelson Ching Yuan
author_facet	Deborah A Ferrington Heidi Roehrich Angela A Chang Craig W Huang Marcela Maldonado Wendy Bratten Abrar A Rageh Neal D Heuss Dale S Gregerson Elizabeth F Nelson Ching Yuan
author_sort	Deborah A Ferrington
title	Corneal wound healing is compromised by immunoproteasome deficiency.
title_short	Corneal wound healing is compromised by immunoproteasome deficiency.
title_full	Corneal wound healing is compromised by immunoproteasome deficiency.
title_fullStr	Corneal wound healing is compromised by immunoproteasome deficiency.
title_full_unstemmed	Corneal wound healing is compromised by immunoproteasome deficiency.
title_sort	corneal wound healing is compromised by immunoproteasome deficiency.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/037c6e22685d48b79d733ac595a54f20
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Corneal wound healing is compromised by immunoproteasome deficiency.

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