TRPA1 triggers hyperalgesia and inflammation after tooth bleaching

Abstract Hyperalgesia has become a major problem restricting the clinical application of tooth bleaching. We hypothesized that transient receptor potential ankyrin 1 (TRPA1), a pain conduction tunnel, plays a role in tooth hyperalgesia and inflammation after bleaching. Dental pulp stem cells were se...

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Autores principales:	Chang Chen, Xiansheng Huang, Wenqiang Zhu, Chen Ding, Piaopiao Huang, Rong Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/037cc3d7c11f4d5cacd95a255881e0ac
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spelling	oai:doaj.org-article:037cc3d7c11f4d5cacd95a255881e0ac2021-12-02T16:37:37ZTRPA1 triggers hyperalgesia and inflammation after tooth bleaching10.1038/s41598-021-97040-w2045-2322https://doaj.org/article/037cc3d7c11f4d5cacd95a255881e0ac2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97040-whttps://doaj.org/toc/2045-2322Abstract Hyperalgesia has become a major problem restricting the clinical application of tooth bleaching. We hypothesized that transient receptor potential ankyrin 1 (TRPA1), a pain conduction tunnel, plays a role in tooth hyperalgesia and inflammation after bleaching. Dental pulp stem cells were seeded on the dentin side of the disc, which was cut from the premolar buccal tissue, with 15% (90 min) or 40% (3 × 15 min) bleaching gel applied on the enamel side, and treated with or without a TRPA1 inhibitor. The bleaching gel stimulated intracellular reactive oxygen species, Ca2+, ATP, and extracellular ATP in a dose-dependent manner, and increased the mRNA and protein levels of hyperalgesia (TRPA1 and PANX1) and inflammation (TNFα and IL6) factors. This increment was adversely affected by TRPA1 inhibitor. In animal study, the protein levels of TRPA1 (P = 0.0006), PANX1 (P < 0.0001), and proliferation factors [PCNA (P < 0.0001) and Caspase 3 (P = 0.0066)] increased significantly after treated rat incisors with 15% and 40% bleaching gels as detected by immunohistochemistry. These results show that TRPA1 plays a critical role in sensitivity and inflammation after tooth bleaching, providing a solid foundation for further research on reducing the complications of tooth bleaching.Chang ChenXiansheng HuangWenqiang ZhuChen DingPiaopiao HuangRong LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chang Chen Xiansheng Huang Wenqiang Zhu Chen Ding Piaopiao Huang Rong Li TRPA1 triggers hyperalgesia and inflammation after tooth bleaching
description	Abstract Hyperalgesia has become a major problem restricting the clinical application of tooth bleaching. We hypothesized that transient receptor potential ankyrin 1 (TRPA1), a pain conduction tunnel, plays a role in tooth hyperalgesia and inflammation after bleaching. Dental pulp stem cells were seeded on the dentin side of the disc, which was cut from the premolar buccal tissue, with 15% (90 min) or 40% (3 × 15 min) bleaching gel applied on the enamel side, and treated with or without a TRPA1 inhibitor. The bleaching gel stimulated intracellular reactive oxygen species, Ca2+, ATP, and extracellular ATP in a dose-dependent manner, and increased the mRNA and protein levels of hyperalgesia (TRPA1 and PANX1) and inflammation (TNFα and IL6) factors. This increment was adversely affected by TRPA1 inhibitor. In animal study, the protein levels of TRPA1 (P = 0.0006), PANX1 (P < 0.0001), and proliferation factors [PCNA (P < 0.0001) and Caspase 3 (P = 0.0066)] increased significantly after treated rat incisors with 15% and 40% bleaching gels as detected by immunohistochemistry. These results show that TRPA1 plays a critical role in sensitivity and inflammation after tooth bleaching, providing a solid foundation for further research on reducing the complications of tooth bleaching.
format	article
author	Chang Chen Xiansheng Huang Wenqiang Zhu Chen Ding Piaopiao Huang Rong Li
author_facet	Chang Chen Xiansheng Huang Wenqiang Zhu Chen Ding Piaopiao Huang Rong Li
author_sort	Chang Chen
title	TRPA1 triggers hyperalgesia and inflammation after tooth bleaching
title_short	TRPA1 triggers hyperalgesia and inflammation after tooth bleaching
title_full	TRPA1 triggers hyperalgesia and inflammation after tooth bleaching
title_fullStr	TRPA1 triggers hyperalgesia and inflammation after tooth bleaching
title_full_unstemmed	TRPA1 triggers hyperalgesia and inflammation after tooth bleaching
title_sort	trpa1 triggers hyperalgesia and inflammation after tooth bleaching
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/037cc3d7c11f4d5cacd95a255881e0ac
work_keys_str_mv	AT changchen trpa1triggershyperalgesiaandinflammationaftertoothbleaching AT xianshenghuang trpa1triggershyperalgesiaandinflammationaftertoothbleaching AT wenqiangzhu trpa1triggershyperalgesiaandinflammationaftertoothbleaching AT chending trpa1triggershyperalgesiaandinflammationaftertoothbleaching AT piaopiaohuang trpa1triggershyperalgesiaandinflammationaftertoothbleaching AT rongli trpa1triggershyperalgesiaandinflammationaftertoothbleaching
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TRPA1 triggers hyperalgesia and inflammation after tooth bleaching

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