Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing

Sample index hopping results in various artefacts in multiplexed scRNA-seq experiments. Here, the authors introduce a statistical model to estimate sample index hopping rate in droplet-based scRNA-seq data and show that artifacts can be corrected by purging phantom molecules from the data.

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Detalles Bibliográficos
Autores principales: Rick Farouni, Haig Djambazian, Lorenzo E. Ferri, Jiannis Ragoussis, Hamed S. Najafabadi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/037f529200114ff2b18593e0a1a1f12c
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Sumario:Sample index hopping results in various artefacts in multiplexed scRNA-seq experiments. Here, the authors introduce a statistical model to estimate sample index hopping rate in droplet-based scRNA-seq data and show that artifacts can be corrected by purging phantom molecules from the data.