Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing
Sample index hopping results in various artefacts in multiplexed scRNA-seq experiments. Here, the authors introduce a statistical model to estimate sample index hopping rate in droplet-based scRNA-seq data and show that artifacts can be corrected by purging phantom molecules from the data.
Guardado en:
Autores principales: | , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2020
|
Materias: | |
Acceso en línea: | https://doaj.org/article/037f529200114ff2b18593e0a1a1f12c |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:037f529200114ff2b18593e0a1a1f12c |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:037f529200114ff2b18593e0a1a1f12c2021-12-02T15:02:47ZModel-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing10.1038/s41467-020-16522-z2041-1723https://doaj.org/article/037f529200114ff2b18593e0a1a1f12c2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16522-zhttps://doaj.org/toc/2041-1723Sample index hopping results in various artefacts in multiplexed scRNA-seq experiments. Here, the authors introduce a statistical model to estimate sample index hopping rate in droplet-based scRNA-seq data and show that artifacts can be corrected by purging phantom molecules from the data.Rick FarouniHaig DjambazianLorenzo E. FerriJiannis RagoussisHamed S. NajafabadiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Science Q |
spellingShingle |
Science Q Rick Farouni Haig Djambazian Lorenzo E. Ferri Jiannis Ragoussis Hamed S. Najafabadi Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing |
description |
Sample index hopping results in various artefacts in multiplexed scRNA-seq experiments. Here, the authors introduce a statistical model to estimate sample index hopping rate in droplet-based scRNA-seq data and show that artifacts can be corrected by purging phantom molecules from the data. |
format |
article |
author |
Rick Farouni Haig Djambazian Lorenzo E. Ferri Jiannis Ragoussis Hamed S. Najafabadi |
author_facet |
Rick Farouni Haig Djambazian Lorenzo E. Ferri Jiannis Ragoussis Hamed S. Najafabadi |
author_sort |
Rick Farouni |
title |
Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing |
title_short |
Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing |
title_full |
Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing |
title_fullStr |
Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing |
title_full_unstemmed |
Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing |
title_sort |
model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell rna-sequencing |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/037f529200114ff2b18593e0a1a1f12c |
work_keys_str_mv |
AT rickfarouni modelbasedanalysisofsampleindexhoppingrevealsitswidespreadartifactsinmultiplexedsinglecellrnasequencing AT haigdjambazian modelbasedanalysisofsampleindexhoppingrevealsitswidespreadartifactsinmultiplexedsinglecellrnasequencing AT lorenzoeferri modelbasedanalysisofsampleindexhoppingrevealsitswidespreadartifactsinmultiplexedsinglecellrnasequencing AT jiannisragoussis modelbasedanalysisofsampleindexhoppingrevealsitswidespreadartifactsinmultiplexedsinglecellrnasequencing AT hamedsnajafabadi modelbasedanalysisofsampleindexhoppingrevealsitswidespreadartifactsinmultiplexedsinglecellrnasequencing |
_version_ |
1718389057361281024 |