Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

Abstract Protein elicitors can induce plant systemic resistance to pathogens. In an earlier study, we cloned an EsxA gene from the plant growth-promoting rhizobacterium Paenibacillus terrae NK3-4 and expressed it in Pichia pastoris. In addition to being important for the pathogenicity of animal path...

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Autores principales:	Wen Qing Yu, Peng Li, Feng Chao Yan, Gui Ping Zheng, Wen Zhi Liu, Wen Xi Lin, Yi Wang, Zhi Qing Luo
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Protein elicitor EsxA Induced systemic resistance Transcriptome Pathogenesis-related protein Signal transduction Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0382385a4fb0489ab333d5d36def6e95
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spelling	oai:doaj.org-article:0382385a4fb0489ab333d5d36def6e952021-11-08T11:15:22ZProtein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice10.1186/s12284-021-00532-61939-84251939-8433https://doaj.org/article/0382385a4fb0489ab333d5d36def6e952021-11-01T00:00:00Zhttps://doi.org/10.1186/s12284-021-00532-6https://doaj.org/toc/1939-8425https://doaj.org/toc/1939-8433Abstract Protein elicitors can induce plant systemic resistance to pathogens. In an earlier study, we cloned an EsxA gene from the plant growth-promoting rhizobacterium Paenibacillus terrae NK3-4 and expressed it in Pichia pastoris. In addition to being important for the pathogenicity of animal pathogens, EsxA can also induce an immune response in animals. While, we found the exogenously expressed EsxA has the activity of elicitor, which can trigger hypersensitive response and reactive oxygen species burst in leaves as well as enhanced rice plant growth. The effects of EsxA on seedling blight (Fusarium oxysporum) resistance and gene transcription, including pathogenesis-related (PR) genes in rice were evaluated. The germination rate was 95.0% for seeds treated with EsxA and then inoculated with F. oxysporum, which was 2.8-times higher than that of F. oxysporum-infected control seeds that were not treated with EsxA (Con). The buds and roots of EsxA-treated seedlings were 2.4- and 15.9-times longer than those of Con seedlings. The plants and roots of seedlings dipped in an EsxA solution and then inoculated with F. oxysporum were longer than those of the Con seedlings. Theplant length, number of total roots, and number of white roots were respectively 23.2%, 1.74-times, and 7.42-times greater for the seedlings sprayed with EsxA and then inoculated with F. oxysporum than for the Con seedlings. The EsxA induction efficiency (spray treatment) on seedling blight resistance was 60.9%. The transcriptome analysis revealed 1137 and 239 rice genes with EsxA-induced up-regulated and down-regulated transcription levels, respectively. At 48 h after the EsxA treatment, the transcription of 611 and 160 genes was up-regulated and down-regulated, respectively, compared with the transcription levels for the untreated control at the same time-point. Many disease resistance-related PR genes had up-regulated transcription levels. The qPCR data were consistent with the transcriptome sequencing results. EsxA triggered rice ISR to seedling blight and gene differential transcription, including the up-regulated transcription of rice PR genes. These findings may be relevant for the use of EsxA as a protein elicitor to control plant diseases.Wen Qing YuPeng LiFeng Chao YanGui Ping ZhengWen Zhi LiuWen Xi LinYi WangZhi Qing LuoSpringerOpenarticleProtein elicitorEsxAInduced systemic resistanceTranscriptomePathogenesis-related proteinSignal transductionPlant cultureSB1-1110ENRice, Vol 14, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Protein elicitor EsxA Induced systemic resistance Transcriptome Pathogenesis-related protein Signal transduction Plant culture SB1-1110
spellingShingle	Protein elicitor EsxA Induced systemic resistance Transcriptome Pathogenesis-related protein Signal transduction Plant culture SB1-1110 Wen Qing Yu Peng Li Feng Chao Yan Gui Ping Zheng Wen Zhi Liu Wen Xi Lin Yi Wang Zhi Qing Luo Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice
description	Abstract Protein elicitors can induce plant systemic resistance to pathogens. In an earlier study, we cloned an EsxA gene from the plant growth-promoting rhizobacterium Paenibacillus terrae NK3-4 and expressed it in Pichia pastoris. In addition to being important for the pathogenicity of animal pathogens, EsxA can also induce an immune response in animals. While, we found the exogenously expressed EsxA has the activity of elicitor, which can trigger hypersensitive response and reactive oxygen species burst in leaves as well as enhanced rice plant growth. The effects of EsxA on seedling blight (Fusarium oxysporum) resistance and gene transcription, including pathogenesis-related (PR) genes in rice were evaluated. The germination rate was 95.0% for seeds treated with EsxA and then inoculated with F. oxysporum, which was 2.8-times higher than that of F. oxysporum-infected control seeds that were not treated with EsxA (Con). The buds and roots of EsxA-treated seedlings were 2.4- and 15.9-times longer than those of Con seedlings. The plants and roots of seedlings dipped in an EsxA solution and then inoculated with F. oxysporum were longer than those of the Con seedlings. Theplant length, number of total roots, and number of white roots were respectively 23.2%, 1.74-times, and 7.42-times greater for the seedlings sprayed with EsxA and then inoculated with F. oxysporum than for the Con seedlings. The EsxA induction efficiency (spray treatment) on seedling blight resistance was 60.9%. The transcriptome analysis revealed 1137 and 239 rice genes with EsxA-induced up-regulated and down-regulated transcription levels, respectively. At 48 h after the EsxA treatment, the transcription of 611 and 160 genes was up-regulated and down-regulated, respectively, compared with the transcription levels for the untreated control at the same time-point. Many disease resistance-related PR genes had up-regulated transcription levels. The qPCR data were consistent with the transcriptome sequencing results. EsxA triggered rice ISR to seedling blight and gene differential transcription, including the up-regulated transcription of rice PR genes. These findings may be relevant for the use of EsxA as a protein elicitor to control plant diseases.
format	article
author	Wen Qing Yu Peng Li Feng Chao Yan Gui Ping Zheng Wen Zhi Liu Wen Xi Lin Yi Wang Zhi Qing Luo
author_facet	Wen Qing Yu Peng Li Feng Chao Yan Gui Ping Zheng Wen Zhi Liu Wen Xi Lin Yi Wang Zhi Qing Luo
author_sort	Wen Qing Yu
title	Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice
title_short	Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice
title_full	Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice
title_fullStr	Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice
title_full_unstemmed	Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice
title_sort	protein elicitor esxa induces resistance to seedling blight and pr genes differential transcription in rice
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/0382385a4fb0489ab333d5d36def6e95
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Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

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