Smartphone-based multi-contrast microscope using color-multiplexed illumination

Abstract We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed “color-coded light-emitting-diode (LED) mic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daeseong Jung, Jun-Ho Choi, Soocheol Kim, Suho Ryu, Wonchan Lee, Jong-Seok Lee, Chulmin Joo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/039d8306331d460a80a11f5d602536a3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:039d8306331d460a80a11f5d602536a3
record_format	dspace
spelling	oai:doaj.org-article:039d8306331d460a80a11f5d602536a32021-12-02T15:05:50ZSmartphone-based multi-contrast microscope using color-multiplexed illumination10.1038/s41598-017-07703-w2045-2322https://doaj.org/article/039d8306331d460a80a11f5d602536a32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07703-whttps://doaj.org/toc/2045-2322Abstract We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed “color-coded light-emitting-diode (LED) microscopy (cLEDscope),” in which a specimen is illuminated with a color-coded LED array and light transmitted through the specimen is recorded by a color image sensor. Decomposition of the image into red, green, and blue colors and subsequent computation enable multi-contrast imaging in a single shot. In order to transform a smartphone into a multi-contrast imaging device, we developed an add-on module composed of a patterned color micro-LED array, specimen stage, and miniature objective. Simple installation of this module onto a smartphone enables multi-contrast imaging of transparent specimens. In addition, an Android-based app was implemented to acquire an image, perform the associated computation, and display the multi-contrast images in real time. Herein, the details of our smartphone module and experimental demonstrations with various biological specimens are presented.Daeseong JungJun-Ho ChoiSoocheol KimSuho RyuWonchan LeeJong-Seok LeeChulmin JooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daeseong Jung Jun-Ho Choi Soocheol Kim Suho Ryu Wonchan Lee Jong-Seok Lee Chulmin Joo Smartphone-based multi-contrast microscope using color-multiplexed illumination
description	Abstract We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed “color-coded light-emitting-diode (LED) microscopy (cLEDscope),” in which a specimen is illuminated with a color-coded LED array and light transmitted through the specimen is recorded by a color image sensor. Decomposition of the image into red, green, and blue colors and subsequent computation enable multi-contrast imaging in a single shot. In order to transform a smartphone into a multi-contrast imaging device, we developed an add-on module composed of a patterned color micro-LED array, specimen stage, and miniature objective. Simple installation of this module onto a smartphone enables multi-contrast imaging of transparent specimens. In addition, an Android-based app was implemented to acquire an image, perform the associated computation, and display the multi-contrast images in real time. Herein, the details of our smartphone module and experimental demonstrations with various biological specimens are presented.
format	article
author	Daeseong Jung Jun-Ho Choi Soocheol Kim Suho Ryu Wonchan Lee Jong-Seok Lee Chulmin Joo
author_facet	Daeseong Jung Jun-Ho Choi Soocheol Kim Suho Ryu Wonchan Lee Jong-Seok Lee Chulmin Joo
author_sort	Daeseong Jung
title	Smartphone-based multi-contrast microscope using color-multiplexed illumination
title_short	Smartphone-based multi-contrast microscope using color-multiplexed illumination
title_full	Smartphone-based multi-contrast microscope using color-multiplexed illumination
title_fullStr	Smartphone-based multi-contrast microscope using color-multiplexed illumination
title_full_unstemmed	Smartphone-based multi-contrast microscope using color-multiplexed illumination
title_sort	smartphone-based multi-contrast microscope using color-multiplexed illumination
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/039d8306331d460a80a11f5d602536a3
work_keys_str_mv	AT daeseongjung smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination AT junhochoi smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination AT soocheolkim smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination AT suhoryu smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination AT wonchanlee smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination AT jongseoklee smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination AT chulminjoo smartphonebasedmulticontrastmicroscopeusingcolormultiplexedillumination
_version_	1718388684813762560

Smartphone-based multi-contrast microscope using color-multiplexed illumination

Ejemplares similares