Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions

In wheat crop the leaf rust is an infectious disease, threatening wheat production annually. Identification of resistance genes or markers traits associations for effective field resistance could greatly enhance our ability to breed durably resistant varieties. Herein 105 bread wheat genotypes were...

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Autores principales:	Hafiz Ghulam Muhu-Din Ahmed, Muhammad Nouman Iqbal, Muhammad Arslan Iqbal, Yawen Zeng, Aziz Ullah, Muhammad Iqbal, Humayun Raza, Muhammad Majid Yar, Hafiz Arslan Anwaar, Noosheen Zahid, Ashir Masroor, Rao Muhammad Ikram
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Genome Disease Fungal Rust Markers Chromosome Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/03a4da57b43f4256b6fa13a28c6e147c
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spelling	oai:doaj.org-article:03a4da57b43f4256b6fa13a28c6e147c2021-11-18T04:44:11ZGenome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions1018-364710.1016/j.jksus.2021.101628https://doaj.org/article/03a4da57b43f4256b6fa13a28c6e147c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721002901https://doaj.org/toc/1018-3647In wheat crop the leaf rust is an infectious disease, threatening wheat production annually. Identification of resistance genes or markers traits associations for effective field resistance could greatly enhance our ability to breed durably resistant varieties. Herein 105 bread wheat genotypes were evaluated against the leaf rust susceptible and resistance during the two seasons 2018–19 and 2019–20 under field conditions using CI, AUDPC and FDS parameters. Based on the performance of genotypes against leaf rust pathogen, 34 genotypes categorised as resistance (R), followed by 30, 32 and 9 genotypes classified as moderately resistance (MR), moderately susceptible (MS) and susceptible (S) genotypes. respectively. We also applied a genome wide association study (GWAS) approach to identify markers trait associations (MTAs) resistance against leaf rust using 90 K SNP Array. Marker–trait association results indicated that total 56 MTAs were significantly associated at P ≤ 10−3 using Bonferroni adjustment after crossed FDR ≤ 0.05 with studied parameters under field conditions. Among them, total numbers of associated MTAs 17, 23 and 16 were identified for CI, AUDPC and FDS parameters, respectively. Maximum significant MTAs were identified on chromosomes 2A (7), 3B (7), 5A (7) followed by 4A (5), 7B (5), 1A (4) and 7A (4) in the studied genotypes. The locus (Kukri_c55051_414) showed pleotropic effects for leaf rust identified parameters on chromosome 5A at position of 68.2 cM under field conditions. This study demonstrated the use of genome-wide association mapping for the identification of potentially new genomic regions associated with studied parameters. Leaf rust resistant genotypes identified in this study proved to be more productive under preliminary field conditions against leaf rust pathogen. Thus, these resistant accessions could be used as parental genotypes in breeding programs for leaf rust resistance.Hafiz Ghulam Muhu-Din AhmedMuhammad Nouman IqbalMuhammad Arslan IqbalYawen ZengAziz UllahMuhammad IqbalHumayun RazaMuhammad Majid YarHafiz Arslan AnwaarNoosheen ZahidAshir MasroorRao Muhammad IkramElsevierarticleGenomeDiseaseFungalRustMarkersChromosomeScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101628- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genome Disease Fungal Rust Markers Chromosome Science (General) Q1-390
spellingShingle	Genome Disease Fungal Rust Markers Chromosome Science (General) Q1-390 Hafiz Ghulam Muhu-Din Ahmed Muhammad Nouman Iqbal Muhammad Arslan Iqbal Yawen Zeng Aziz Ullah Muhammad Iqbal Humayun Raza Muhammad Majid Yar Hafiz Arslan Anwaar Noosheen Zahid Ashir Masroor Rao Muhammad Ikram Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions
description	In wheat crop the leaf rust is an infectious disease, threatening wheat production annually. Identification of resistance genes or markers traits associations for effective field resistance could greatly enhance our ability to breed durably resistant varieties. Herein 105 bread wheat genotypes were evaluated against the leaf rust susceptible and resistance during the two seasons 2018–19 and 2019–20 under field conditions using CI, AUDPC and FDS parameters. Based on the performance of genotypes against leaf rust pathogen, 34 genotypes categorised as resistance (R), followed by 30, 32 and 9 genotypes classified as moderately resistance (MR), moderately susceptible (MS) and susceptible (S) genotypes. respectively. We also applied a genome wide association study (GWAS) approach to identify markers trait associations (MTAs) resistance against leaf rust using 90 K SNP Array. Marker–trait association results indicated that total 56 MTAs were significantly associated at P ≤ 10−3 using Bonferroni adjustment after crossed FDR ≤ 0.05 with studied parameters under field conditions. Among them, total numbers of associated MTAs 17, 23 and 16 were identified for CI, AUDPC and FDS parameters, respectively. Maximum significant MTAs were identified on chromosomes 2A (7), 3B (7), 5A (7) followed by 4A (5), 7B (5), 1A (4) and 7A (4) in the studied genotypes. The locus (Kukri_c55051_414) showed pleotropic effects for leaf rust identified parameters on chromosome 5A at position of 68.2 cM under field conditions. This study demonstrated the use of genome-wide association mapping for the identification of potentially new genomic regions associated with studied parameters. Leaf rust resistant genotypes identified in this study proved to be more productive under preliminary field conditions against leaf rust pathogen. Thus, these resistant accessions could be used as parental genotypes in breeding programs for leaf rust resistance.
format	article
author	Hafiz Ghulam Muhu-Din Ahmed Muhammad Nouman Iqbal Muhammad Arslan Iqbal Yawen Zeng Aziz Ullah Muhammad Iqbal Humayun Raza Muhammad Majid Yar Hafiz Arslan Anwaar Noosheen Zahid Ashir Masroor Rao Muhammad Ikram
author_facet	Hafiz Ghulam Muhu-Din Ahmed Muhammad Nouman Iqbal Muhammad Arslan Iqbal Yawen Zeng Aziz Ullah Muhammad Iqbal Humayun Raza Muhammad Majid Yar Hafiz Arslan Anwaar Noosheen Zahid Ashir Masroor Rao Muhammad Ikram
author_sort	Hafiz Ghulam Muhu-Din Ahmed
title	Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions
title_short	Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions
title_full	Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions
title_fullStr	Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions
title_full_unstemmed	Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions
title_sort	genome wide association mapping through 90k snp array against leaf rust pathogen in bread wheat genotypes under field conditions
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/03a4da57b43f4256b6fa13a28c6e147c
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Genome wide association mapping through 90K SNP array against leaf rust pathogen in bread wheat genotypes under field conditions

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