Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

Laser capture microscopy (LCM) coupled with global transcriptome profiling requires relatively large numbers of cells. Here, the authors show that LCM coupled with full-length mRNA-sequencing (LCM-seq) can sequence single cells, and that LCM-seq can provide biological insight on highly similar neuro...

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Autores principales:	Susanne Nichterwitz, Geng Chen, Julio Aguila Benitez, Marlene Yilmaz, Helena Storvall, Ming Cao, Rickard Sandberg, Qiaolin Deng, Eva Hedlund
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/03a83be9ece74bdda6b628a6297aba9b
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spelling	oai:doaj.org-article:03a83be9ece74bdda6b628a6297aba9b2021-12-02T15:34:54ZLaser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling10.1038/ncomms121392041-1723https://doaj.org/article/03a83be9ece74bdda6b628a6297aba9b2016-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms12139https://doaj.org/toc/2041-1723Laser capture microscopy (LCM) coupled with global transcriptome profiling requires relatively large numbers of cells. Here, the authors show that LCM coupled with full-length mRNA-sequencing (LCM-seq) can sequence single cells, and that LCM-seq can provide biological insight on highly similar neuronal populations.Susanne NichterwitzGeng ChenJulio Aguila BenitezMarlene YilmazHelena StorvallMing CaoRickard SandbergQiaolin DengEva HedlundNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Susanne Nichterwitz Geng Chen Julio Aguila Benitez Marlene Yilmaz Helena Storvall Ming Cao Rickard Sandberg Qiaolin Deng Eva Hedlund Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
description	Laser capture microscopy (LCM) coupled with global transcriptome profiling requires relatively large numbers of cells. Here, the authors show that LCM coupled with full-length mRNA-sequencing (LCM-seq) can sequence single cells, and that LCM-seq can provide biological insight on highly similar neuronal populations.
format	article
author	Susanne Nichterwitz Geng Chen Julio Aguila Benitez Marlene Yilmaz Helena Storvall Ming Cao Rickard Sandberg Qiaolin Deng Eva Hedlund
author_facet	Susanne Nichterwitz Geng Chen Julio Aguila Benitez Marlene Yilmaz Helena Storvall Ming Cao Rickard Sandberg Qiaolin Deng Eva Hedlund
author_sort	Susanne Nichterwitz
title	Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
title_short	Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
title_full	Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
title_fullStr	Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
title_full_unstemmed	Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
title_sort	laser capture microscopy coupled with smart-seq2 for precise spatial transcriptomic profiling
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/03a83be9ece74bdda6b628a6297aba9b
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Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

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