Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β

Abstract Multiple sclerosis (MS) is a neurodegenerative autoimmune disease, where chronic inflammation plays an essential role in its pathology. A feature of MS is the production of autoantibodies stimulated by an altered-peptide-ligand response and epitope spreading, resulting in loss of tolerance...

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Autores principales:	Anne Skøttrup Mørkholt, Kenneth Kastaniegaard, Michael Sloth Trabjerg, Gopana Gopalasingam, Wanda Niganze, Agnete Larsen, Allan Stensballe, Søren Nielsen, John Dirk Nieland
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03ac8250d29948be8bb7fde068569474
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spelling	oai:doaj.org-article:03ac8250d29948be8bb7fde0685694742021-12-02T16:07:51ZIdentification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β10.1038/s41598-018-25391-y2045-2322https://doaj.org/article/03ac8250d29948be8bb7fde0685694742018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25391-yhttps://doaj.org/toc/2045-2322Abstract Multiple sclerosis (MS) is a neurodegenerative autoimmune disease, where chronic inflammation plays an essential role in its pathology. A feature of MS is the production of autoantibodies stimulated by an altered-peptide-ligand response and epitope spreading, resulting in loss of tolerance for self-proteins. The involvement of autoantibodies in MS pathogenesis has been suggested to initiate and drive progression of inflammation; however, the etiology of MS remains unknown. The effect of etomoxir and interferon-β (IFN-β) was examined in an experimental-autoimmune-encephalomyelitis (EAE) model of MS. Moreover, the impact of etomoxir and IFN-β on recognition of brain proteins in serum from EAE rats was examined with the purpose of identifying the autoantibody reactivities involved in MS. Animals treated with etomoxir on day 1 exhibited a statistically significantly lower disease score than animals treated with IFN-β (on day 1 or 5) or placebo. Etomoxir treatment on day 5 resulted in a significantly lower disease score than IFN-β treatment on day 1. After disease induction antibodies was induced to a broad pallet of antigens in the brain. Surprisingly, by blocking CPT1 and therewith lipid metabolism several alterations in the antibody response was observed suggesting that autoantibodies play a role in the EAE animal model.Anne Skøttrup MørkholtKenneth KastaniegaardMichael Sloth TrabjergGopana GopalasingamWanda NiganzeAgnete LarsenAllan StensballeSøren NielsenJohn Dirk NielandNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne Skøttrup Mørkholt Kenneth Kastaniegaard Michael Sloth Trabjerg Gopana Gopalasingam Wanda Niganze Agnete Larsen Allan Stensballe Søren Nielsen John Dirk Nieland Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
description	Abstract Multiple sclerosis (MS) is a neurodegenerative autoimmune disease, where chronic inflammation plays an essential role in its pathology. A feature of MS is the production of autoantibodies stimulated by an altered-peptide-ligand response and epitope spreading, resulting in loss of tolerance for self-proteins. The involvement of autoantibodies in MS pathogenesis has been suggested to initiate and drive progression of inflammation; however, the etiology of MS remains unknown. The effect of etomoxir and interferon-β (IFN-β) was examined in an experimental-autoimmune-encephalomyelitis (EAE) model of MS. Moreover, the impact of etomoxir and IFN-β on recognition of brain proteins in serum from EAE rats was examined with the purpose of identifying the autoantibody reactivities involved in MS. Animals treated with etomoxir on day 1 exhibited a statistically significantly lower disease score than animals treated with IFN-β (on day 1 or 5) or placebo. Etomoxir treatment on day 5 resulted in a significantly lower disease score than IFN-β treatment on day 1. After disease induction antibodies was induced to a broad pallet of antigens in the brain. Surprisingly, by blocking CPT1 and therewith lipid metabolism several alterations in the antibody response was observed suggesting that autoantibodies play a role in the EAE animal model.
format	article
author	Anne Skøttrup Mørkholt Kenneth Kastaniegaard Michael Sloth Trabjerg Gopana Gopalasingam Wanda Niganze Agnete Larsen Allan Stensballe Søren Nielsen John Dirk Nieland
author_facet	Anne Skøttrup Mørkholt Kenneth Kastaniegaard Michael Sloth Trabjerg Gopana Gopalasingam Wanda Niganze Agnete Larsen Allan Stensballe Søren Nielsen John Dirk Nieland
author_sort	Anne Skøttrup Mørkholt
title	Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
title_short	Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
title_full	Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
title_fullStr	Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
title_full_unstemmed	Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
title_sort	identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/03ac8250d29948be8bb7fde068569474
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Identification of brain antigens recognized by autoantibodies in experimental autoimmune encephalomyelitis-induced animals treated with etomoxir or interferon-β

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