Immunomodulation stimulates the innervation of engineered tooth organ.

The sensory innervation of the dental mesenchyme is essential for tooth function and protection. Sensory innervation of the dental pulp is mediated by axons originating from the trigeminal ganglia and is strictly regulated in time. Teeth can develop from cultured re-associations between dissociated...

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Autores principales:	Tunay Kökten, Thibault Bécavin, Laetitia Keller, Jean-Luc Weickert, Sabine Kuchler-Bopp, Hervé Lesot
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03ae434479db45d5bbcd763e06f93fa3
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spelling	oai:doaj.org-article:03ae434479db45d5bbcd763e06f93fa32021-11-18T08:36:31ZImmunomodulation stimulates the innervation of engineered tooth organ.1932-620310.1371/journal.pone.0086011https://doaj.org/article/03ae434479db45d5bbcd763e06f93fa32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465840/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The sensory innervation of the dental mesenchyme is essential for tooth function and protection. Sensory innervation of the dental pulp is mediated by axons originating from the trigeminal ganglia and is strictly regulated in time. Teeth can develop from cultured re-associations between dissociated dental epithelial and mesenchymal cells from Embryonic Day 14 mouse molars, after implantation under the skin of adult ICR mice. In these conditions however, the innervation of the dental mesenchyme did not occur spontaneously. In order to go further with this question, complementary experimental approaches were designed. Cultured cell re-associations were implanted together with trigeminal ganglia for one or two weeks. Although axonal growth was regularly observed extending from the trigeminal ganglia to all around the forming teeth, the presence of axons in the dental mesenchyme was detected in less than 2.5% of samples after two weeks, demonstrating a specific impairment of their entering the dental mesenchyme. In clinical context, immunosuppressive therapy using cyclosporin A was found to accelerate the innervation of transplanted tissues. Indeed, when cultured cell re-associations and trigeminal ganglia were co-implanted in cyclosporin A-treated ICR mice, nerve fibers were detected in the dental pulp, even reaching odontoblasts after one week. However, cyclosporin A shows multiple effects, including direct ones on nerve growth. To test whether there may be a direct functional relationship between immunomodulation and innervation, cell re-associations and trigeminal ganglia were co-implanted in immunocompromised Nude mice. In these conditions as well, the innervation of the dental mesenchyme was observed already after one week of implantation, but axons reached the odontoblast layer after two weeks only. This study demonstrated that immunodepression per se does stimulate the innervation of the dental mesenchyme.Tunay KöktenThibault BécavinLaetitia KellerJean-Luc WeickertSabine Kuchler-BoppHervé LesotPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86011 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tunay Kökten Thibault Bécavin Laetitia Keller Jean-Luc Weickert Sabine Kuchler-Bopp Hervé Lesot Immunomodulation stimulates the innervation of engineered tooth organ.
description	The sensory innervation of the dental mesenchyme is essential for tooth function and protection. Sensory innervation of the dental pulp is mediated by axons originating from the trigeminal ganglia and is strictly regulated in time. Teeth can develop from cultured re-associations between dissociated dental epithelial and mesenchymal cells from Embryonic Day 14 mouse molars, after implantation under the skin of adult ICR mice. In these conditions however, the innervation of the dental mesenchyme did not occur spontaneously. In order to go further with this question, complementary experimental approaches were designed. Cultured cell re-associations were implanted together with trigeminal ganglia for one or two weeks. Although axonal growth was regularly observed extending from the trigeminal ganglia to all around the forming teeth, the presence of axons in the dental mesenchyme was detected in less than 2.5% of samples after two weeks, demonstrating a specific impairment of their entering the dental mesenchyme. In clinical context, immunosuppressive therapy using cyclosporin A was found to accelerate the innervation of transplanted tissues. Indeed, when cultured cell re-associations and trigeminal ganglia were co-implanted in cyclosporin A-treated ICR mice, nerve fibers were detected in the dental pulp, even reaching odontoblasts after one week. However, cyclosporin A shows multiple effects, including direct ones on nerve growth. To test whether there may be a direct functional relationship between immunomodulation and innervation, cell re-associations and trigeminal ganglia were co-implanted in immunocompromised Nude mice. In these conditions as well, the innervation of the dental mesenchyme was observed already after one week of implantation, but axons reached the odontoblast layer after two weeks only. This study demonstrated that immunodepression per se does stimulate the innervation of the dental mesenchyme.
format	article
author	Tunay Kökten Thibault Bécavin Laetitia Keller Jean-Luc Weickert Sabine Kuchler-Bopp Hervé Lesot
author_facet	Tunay Kökten Thibault Bécavin Laetitia Keller Jean-Luc Weickert Sabine Kuchler-Bopp Hervé Lesot
author_sort	Tunay Kökten
title	Immunomodulation stimulates the innervation of engineered tooth organ.
title_short	Immunomodulation stimulates the innervation of engineered tooth organ.
title_full	Immunomodulation stimulates the innervation of engineered tooth organ.
title_fullStr	Immunomodulation stimulates the innervation of engineered tooth organ.
title_full_unstemmed	Immunomodulation stimulates the innervation of engineered tooth organ.
title_sort	immunomodulation stimulates the innervation of engineered tooth organ.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/03ae434479db45d5bbcd763e06f93fa3
work_keys_str_mv	AT tunaykokten immunomodulationstimulatestheinnervationofengineeredtoothorgan AT thibaultbecavin immunomodulationstimulatestheinnervationofengineeredtoothorgan AT laetitiakeller immunomodulationstimulatestheinnervationofengineeredtoothorgan AT jeanlucweickert immunomodulationstimulatestheinnervationofengineeredtoothorgan AT sabinekuchlerbopp immunomodulationstimulatestheinnervationofengineeredtoothorgan AT hervelesot immunomodulationstimulatestheinnervationofengineeredtoothorgan
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Immunomodulation stimulates the innervation of engineered tooth organ.

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