Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus

African swine fever virus (ASFV), causing an OIE-notifiable viral disease of swine, is spreading over the Eurasian continent and threatening the global pig industry. Here, we conducted the first proteome analysis of ASFV-infected primary porcine monocyte-derived macrophages (moMΦ). In parallel to mo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elisabeth Wöhnke, Walter Fuchs, Luise Hartmann, Ulrike Blohm, Sandra Blome, Thomas C. Mettenleiter, Axel Karger
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	African swine fever virus proteome primary cell culture mass spectrometry Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/03afd1cb0e0b42328f6204e806c1e3d2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:03afd1cb0e0b42328f6204e806c1e3d2
record_format	dspace
spelling	oai:doaj.org-article:03afd1cb0e0b42328f6204e806c1e3d22021-11-25T19:13:16ZComparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus10.3390/v131121981999-4915https://doaj.org/article/03afd1cb0e0b42328f6204e806c1e3d22021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2198https://doaj.org/toc/1999-4915African swine fever virus (ASFV), causing an OIE-notifiable viral disease of swine, is spreading over the Eurasian continent and threatening the global pig industry. Here, we conducted the first proteome analysis of ASFV-infected primary porcine monocyte-derived macrophages (moMΦ). In parallel to moMΦ isolated from different pigs, the stable porcine cell line WSL-R was infected with a recombinant of ASFV genotype IX strain “Kenya1033”. The outcome of the infections was compared via quantitative mass spectrometry (MS)-based proteome analysis. Major differences with respect to the expression of viral proteins or the host cell response were not observed. However, cell-specific expression of some individual viral proteins did occur. The observed modulations of the host proteome were mainly related to cell characteristics and function. Overall, we conclude that both infection models are suitable for use in the study of ASFV infection in vitro.Elisabeth WöhnkeWalter FuchsLuise HartmannUlrike BlohmSandra BlomeThomas C. MettenleiterAxel KargerMDPI AGarticleAfrican swine fever virusproteomeprimary cell culturemass spectrometryMicrobiologyQR1-502ENViruses, Vol 13, Iss 2198, p 2198 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	African swine fever virus proteome primary cell culture mass spectrometry Microbiology QR1-502
spellingShingle	African swine fever virus proteome primary cell culture mass spectrometry Microbiology QR1-502 Elisabeth Wöhnke Walter Fuchs Luise Hartmann Ulrike Blohm Sandra Blome Thomas C. Mettenleiter Axel Karger Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus
description	African swine fever virus (ASFV), causing an OIE-notifiable viral disease of swine, is spreading over the Eurasian continent and threatening the global pig industry. Here, we conducted the first proteome analysis of ASFV-infected primary porcine monocyte-derived macrophages (moMΦ). In parallel to moMΦ isolated from different pigs, the stable porcine cell line WSL-R was infected with a recombinant of ASFV genotype IX strain “Kenya1033”. The outcome of the infections was compared via quantitative mass spectrometry (MS)-based proteome analysis. Major differences with respect to the expression of viral proteins or the host cell response were not observed. However, cell-specific expression of some individual viral proteins did occur. The observed modulations of the host proteome were mainly related to cell characteristics and function. Overall, we conclude that both infection models are suitable for use in the study of ASFV infection in vitro.
format	article
author	Elisabeth Wöhnke Walter Fuchs Luise Hartmann Ulrike Blohm Sandra Blome Thomas C. Mettenleiter Axel Karger
author_facet	Elisabeth Wöhnke Walter Fuchs Luise Hartmann Ulrike Blohm Sandra Blome Thomas C. Mettenleiter Axel Karger
author_sort	Elisabeth Wöhnke
title	Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus
title_short	Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus
title_full	Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus
title_fullStr	Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus
title_full_unstemmed	Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus
title_sort	comparison of the proteomes of porcine macrophages and a stable porcine cell line after infection with african swine fever virus
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/03afd1cb0e0b42328f6204e806c1e3d2
work_keys_str_mv	AT elisabethwohnke comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus AT walterfuchs comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus AT luisehartmann comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus AT ulrikeblohm comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus AT sandrablome comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus AT thomascmettenleiter comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus AT axelkarger comparisonoftheproteomesofporcinemacrophagesandastableporcinecelllineafterinfectionwithafricanswinefevervirus
_version_	1718410133758803968

Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus

Ejemplares similares