Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.

Kv7.2 and Kv7.3 are the main components of the neuronal voltage-dependent M-current, which is a subthreshold potassium conductance that exerts an important control on neuronal excitability. Despite their predominantly intracellular distribution, these channels must reach the plasma membrane in order...

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Autores principales:	Paloma Aivar, Juncal Fernández-Orth, Carolina Gomis-Perez, Araitz Alberdi, Alessandro Alaimo, Manuel S Rodríguez, Teresa Giraldez, Pablo Miranda, Pilar Areso, Alvaro Villarroel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03b2e166e5c6489d990b8c77d87459e1
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spelling	oai:doaj.org-article:03b2e166e5c6489d990b8c77d87459e12021-11-18T08:11:05ZSurface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.1932-620310.1371/journal.pone.0047263https://doaj.org/article/03b2e166e5c6489d990b8c77d87459e12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23115641/?tool=EBIhttps://doaj.org/toc/1932-6203Kv7.2 and Kv7.3 are the main components of the neuronal voltage-dependent M-current, which is a subthreshold potassium conductance that exerts an important control on neuronal excitability. Despite their predominantly intracellular distribution, these channels must reach the plasma membrane in order to control neuronal activity. Thus, we analyzed the amino acid sequence of Kv7.2 to identify intrinsic signals that may control its surface expression. Removal of the interlinker connecting helix A and helix B of the intracellular C-terminus produces a large increase in the number of functional channels at the plasma membrane. Moreover, elimination of this linker increased the steady-state amount of protein, which was not associated with a decrease of protein degradation. The magnitude of this increase was inversely correlated with the number of helix A - helix B linkers present in the tetrameric channel assemblies. In contrast to the remarkable effect on the amount of Kv7.2 protein, removal of the Kv7.2 linker had no detectable impact on the steady-state levels of Kv7.3 protein.Paloma AivarJuncal Fernández-OrthCarolina Gomis-PerezAraitz AlberdiAlessandro AlaimoManuel S RodríguezTeresa GiraldezPablo MirandaPilar AresoAlvaro VillarroelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47263 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paloma Aivar Juncal Fernández-Orth Carolina Gomis-Perez Araitz Alberdi Alessandro Alaimo Manuel S Rodríguez Teresa Giraldez Pablo Miranda Pilar Areso Alvaro Villarroel Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.
description	Kv7.2 and Kv7.3 are the main components of the neuronal voltage-dependent M-current, which is a subthreshold potassium conductance that exerts an important control on neuronal excitability. Despite their predominantly intracellular distribution, these channels must reach the plasma membrane in order to control neuronal activity. Thus, we analyzed the amino acid sequence of Kv7.2 to identify intrinsic signals that may control its surface expression. Removal of the interlinker connecting helix A and helix B of the intracellular C-terminus produces a large increase in the number of functional channels at the plasma membrane. Moreover, elimination of this linker increased the steady-state amount of protein, which was not associated with a decrease of protein degradation. The magnitude of this increase was inversely correlated with the number of helix A - helix B linkers present in the tetrameric channel assemblies. In contrast to the remarkable effect on the amount of Kv7.2 protein, removal of the Kv7.2 linker had no detectable impact on the steady-state levels of Kv7.3 protein.
format	article
author	Paloma Aivar Juncal Fernández-Orth Carolina Gomis-Perez Araitz Alberdi Alessandro Alaimo Manuel S Rodríguez Teresa Giraldez Pablo Miranda Pilar Areso Alvaro Villarroel
author_facet	Paloma Aivar Juncal Fernández-Orth Carolina Gomis-Perez Araitz Alberdi Alessandro Alaimo Manuel S Rodríguez Teresa Giraldez Pablo Miranda Pilar Areso Alvaro Villarroel
author_sort	Paloma Aivar
title	Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.
title_short	Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.
title_full	Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.
title_fullStr	Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.
title_full_unstemmed	Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.
title_sort	surface expression and subunit specific control of steady protein levels by the kv7.2 helix a-b linker.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/03b2e166e5c6489d990b8c77d87459e1
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Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker.

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