Gremlin enhances the determined path to cardiomyogenesis.

<h4>Background</h4>The critical event in heart formation is commitment of mesodermal cells to a cardiomyogenic fate, and cardiac fate determination is regulated by a series of cytokines. Bone morphogenetic proteins (BMPs) and fibroblast growth factors have been shown to be involved in th...

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Autores principales:	Daisuke Kami, Ichiro Shiojima, Hatsune Makino, Kenji Matsumoto, Yoriko Takahashi, Ryuga Ishii, Atsuhiko T Naito, Masashi Toyoda, Hirohisa Saito, Masatoshi Watanabe, Issei Komuro, Akihiro Umezawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03c04e1e16af47eb8a864d3249975e5c
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spelling	oai:doaj.org-article:03c04e1e16af47eb8a864d3249975e5c2021-11-25T06:12:05ZGremlin enhances the determined path to cardiomyogenesis.1932-620310.1371/journal.pone.0002407https://doaj.org/article/03c04e1e16af47eb8a864d3249975e5c2008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18545679/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The critical event in heart formation is commitment of mesodermal cells to a cardiomyogenic fate, and cardiac fate determination is regulated by a series of cytokines. Bone morphogenetic proteins (BMPs) and fibroblast growth factors have been shown to be involved in this process, however additional factors needs to be identified for the fate determination, especially at the early stage of cardiomyogenic development.<h4>Methodology/principal findings</h4>Global gene expression analysis using a series of human cells with a cardiomyogenic potential suggested Gremlin (Grem1) is a candidate gene responsible for in vitro cardiomyogenic differentiation. Grem1, a known BMP antagonist, enhanced DMSO-induced cardiomyogenesis of P19CL6 embryonal carcinoma cells (CL6 cells) 10-35 fold in an area of beating differentiated cardiomyocytes. The Grem1 action was most effective at the early differentiation stage when CL6 cells were destined to cardiomyogenesis, and was mediated through inhibition of BMP2. Furthermore, BMP2 inhibited Wnt/beta-catenin signaling that promoted CL6 cardiomyogenesis.<h4>Conclusions/significance</h4>Grem1 enhances the determined path to cardiomyogenesis in a stage-specific manner, and inhibition of the BMP signaling pathway is involved in initial determination of Grem1-promoted cardiomyogenesis. Our results shed new light on renewal of the cardiovascular system using Grem1 in human.Daisuke KamiIchiro ShiojimaHatsune MakinoKenji MatsumotoYoriko TakahashiRyuga IshiiAtsuhiko T NaitoMasashi ToyodaHirohisa SaitoMasatoshi WatanabeIssei KomuroAkihiro UmezawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 6, p e2407 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daisuke Kami Ichiro Shiojima Hatsune Makino Kenji Matsumoto Yoriko Takahashi Ryuga Ishii Atsuhiko T Naito Masashi Toyoda Hirohisa Saito Masatoshi Watanabe Issei Komuro Akihiro Umezawa Gremlin enhances the determined path to cardiomyogenesis.
description	<h4>Background</h4>The critical event in heart formation is commitment of mesodermal cells to a cardiomyogenic fate, and cardiac fate determination is regulated by a series of cytokines. Bone morphogenetic proteins (BMPs) and fibroblast growth factors have been shown to be involved in this process, however additional factors needs to be identified for the fate determination, especially at the early stage of cardiomyogenic development.<h4>Methodology/principal findings</h4>Global gene expression analysis using a series of human cells with a cardiomyogenic potential suggested Gremlin (Grem1) is a candidate gene responsible for in vitro cardiomyogenic differentiation. Grem1, a known BMP antagonist, enhanced DMSO-induced cardiomyogenesis of P19CL6 embryonal carcinoma cells (CL6 cells) 10-35 fold in an area of beating differentiated cardiomyocytes. The Grem1 action was most effective at the early differentiation stage when CL6 cells were destined to cardiomyogenesis, and was mediated through inhibition of BMP2. Furthermore, BMP2 inhibited Wnt/beta-catenin signaling that promoted CL6 cardiomyogenesis.<h4>Conclusions/significance</h4>Grem1 enhances the determined path to cardiomyogenesis in a stage-specific manner, and inhibition of the BMP signaling pathway is involved in initial determination of Grem1-promoted cardiomyogenesis. Our results shed new light on renewal of the cardiovascular system using Grem1 in human.
format	article
author	Daisuke Kami Ichiro Shiojima Hatsune Makino Kenji Matsumoto Yoriko Takahashi Ryuga Ishii Atsuhiko T Naito Masashi Toyoda Hirohisa Saito Masatoshi Watanabe Issei Komuro Akihiro Umezawa
author_facet	Daisuke Kami Ichiro Shiojima Hatsune Makino Kenji Matsumoto Yoriko Takahashi Ryuga Ishii Atsuhiko T Naito Masashi Toyoda Hirohisa Saito Masatoshi Watanabe Issei Komuro Akihiro Umezawa
author_sort	Daisuke Kami
title	Gremlin enhances the determined path to cardiomyogenesis.
title_short	Gremlin enhances the determined path to cardiomyogenesis.
title_full	Gremlin enhances the determined path to cardiomyogenesis.
title_fullStr	Gremlin enhances the determined path to cardiomyogenesis.
title_full_unstemmed	Gremlin enhances the determined path to cardiomyogenesis.
title_sort	gremlin enhances the determined path to cardiomyogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/03c04e1e16af47eb8a864d3249975e5c
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Gremlin enhances the determined path to cardiomyogenesis.

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