Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip
Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromato...
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Taylor & Francis Group
2018
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oai:doaj.org-article:03cd1538a0874461bc2ebfc49657ec8b2021-11-26T11:19:46ZRapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip0954-01051465-344310.1080/09540105.2018.1460325https://doaj.org/article/03cd1538a0874461bc2ebfc49657ec8b2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1460325https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO.Min FengSteven SuryoprabowoHong TaoLiqiang LiuQiankun ZhengHua KuangTaylor & Francis Grouparticleapraclonidineclonidineimmunochromatographic assaymonoclonal antibodystrip testAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 821-832 (2018) |
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DOAJ |
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DOAJ |
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EN |
| topic |
apraclonidine clonidine immunochromatographic assay monoclonal antibody strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
apraclonidine clonidine immunochromatographic assay monoclonal antibody strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Min Feng Steven Suryoprabowo Hong Tao Liqiang Liu Qiankun Zheng Hua Kuang Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| description |
Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO. |
| format |
article |
| author |
Min Feng Steven Suryoprabowo Hong Tao Liqiang Liu Qiankun Zheng Hua Kuang |
| author_facet |
Min Feng Steven Suryoprabowo Hong Tao Liqiang Liu Qiankun Zheng Hua Kuang |
| author_sort |
Min Feng |
| title |
Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_short |
Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_full |
Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_fullStr |
Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_full_unstemmed |
Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_sort |
rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/03cd1538a0874461bc2ebfc49657ec8b |
| work_keys_str_mv |
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| _version_ |
1718409514532732928 |