An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth.
Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350-320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/03d556d89b264d40979d37393cf16cfb |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:03d556d89b264d40979d37393cf16cfb |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:03d556d89b264d40979d37393cf16cfb2021-12-02T20:06:31ZAn insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth.1932-620310.1371/journal.pone.0255006https://doaj.org/article/03d556d89b264d40979d37393cf16cfb2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255006https://doaj.org/toc/1932-6203Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350-320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene repertoire. Duplicated genes can be retained by different molecular mechanisms such as subfunctionalization, neofunctionalization or redundancy, each one with different functional implications. While the great majority of ohnolog genes have been identified in the teleost genomes, the effect of gene duplication in the fish physiology is still not well characterized. In the present study we studied the effect of WGD on the transcription of the duplicated components controlling muscle growth. We compared the expression of lineage-specific ohnologs related to myogenesis and protein balance in the fast-skeletal muscle of pacus (Piaractus mesopotamicus-Ostariophysi) and Nile tilapias (Oreochromis niloticus-Acanthopterygii) fasted for 4 days and refed for 3 days. We studied the expression of 20 ohnologs and found that in the great majority of cases, duplicated genes had similar expression profiles in response to fasting and refeeding, indicating that their functions during growth have been conserved during the period after the WGD. Our results suggest that redundancy might play a more important role in the retention of ohnologs of regulatory pathways than initially thought. Also, comparison to non-duplicated orthologs showed that it might not be uncommon for the duplicated genes to gain or loss new regulatory elements simultaneously. Overall, several of duplicated ohnologs have similar transcription profiles in response to pro-growth signals suggesting that evolution tends to conserve ohnolog regulation during muscle development and that in the majority of ohnologs related to muscle growth their functions might be very similar.Bruno Oliveira Silva DuranDaniel Garcia de la SerranaBruna Tereza Thomazini ZanellaErika Stefani PerezEdson Assunção MarecoVander Bruno SantosRobson Francisco CarvalhoMaeli Dal-Pai-SilvaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255006 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Bruno Oliveira Silva Duran Daniel Garcia de la Serrana Bruna Tereza Thomazini Zanella Erika Stefani Perez Edson Assunção Mareco Vander Bruno Santos Robson Francisco Carvalho Maeli Dal-Pai-Silva An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| description |
Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350-320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene repertoire. Duplicated genes can be retained by different molecular mechanisms such as subfunctionalization, neofunctionalization or redundancy, each one with different functional implications. While the great majority of ohnolog genes have been identified in the teleost genomes, the effect of gene duplication in the fish physiology is still not well characterized. In the present study we studied the effect of WGD on the transcription of the duplicated components controlling muscle growth. We compared the expression of lineage-specific ohnologs related to myogenesis and protein balance in the fast-skeletal muscle of pacus (Piaractus mesopotamicus-Ostariophysi) and Nile tilapias (Oreochromis niloticus-Acanthopterygii) fasted for 4 days and refed for 3 days. We studied the expression of 20 ohnologs and found that in the great majority of cases, duplicated genes had similar expression profiles in response to fasting and refeeding, indicating that their functions during growth have been conserved during the period after the WGD. Our results suggest that redundancy might play a more important role in the retention of ohnologs of regulatory pathways than initially thought. Also, comparison to non-duplicated orthologs showed that it might not be uncommon for the duplicated genes to gain or loss new regulatory elements simultaneously. Overall, several of duplicated ohnologs have similar transcription profiles in response to pro-growth signals suggesting that evolution tends to conserve ohnolog regulation during muscle development and that in the majority of ohnologs related to muscle growth their functions might be very similar. |
| format |
article |
| author |
Bruno Oliveira Silva Duran Daniel Garcia de la Serrana Bruna Tereza Thomazini Zanella Erika Stefani Perez Edson Assunção Mareco Vander Bruno Santos Robson Francisco Carvalho Maeli Dal-Pai-Silva |
| author_facet |
Bruno Oliveira Silva Duran Daniel Garcia de la Serrana Bruna Tereza Thomazini Zanella Erika Stefani Perez Edson Assunção Mareco Vander Bruno Santos Robson Francisco Carvalho Maeli Dal-Pai-Silva |
| author_sort |
Bruno Oliveira Silva Duran |
| title |
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| title_short |
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| title_full |
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| title_fullStr |
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| title_full_unstemmed |
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| title_sort |
insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/03d556d89b264d40979d37393cf16cfb |
| work_keys_str_mv |
AT brunooliveirasilvaduran aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT danielgarciadelaserrana aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT brunaterezathomazinizanella aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT erikastefaniperez aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT edsonassuncaomareco aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT vanderbrunosantos aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT robsonfranciscocarvalho aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT maelidalpaisilva aninsightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT brunooliveirasilvaduran insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT danielgarciadelaserrana insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT brunaterezathomazinizanella insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT erikastefaniperez insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT edsonassuncaomareco insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT vanderbrunosantos insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT robsonfranciscocarvalho insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth AT maelidalpaisilva insightontheimpactofteleostwholegenomeduplicationontheregulationofthemolecularnetworkscontrollingskeletalmusclegrowth |
| _version_ |
1718375342646755328 |