Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization

Abstract HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis. Studies have shown that the association of Nef with the inner leaflet of the plasma membrane and with endocytic and perinuclear vesicles is essential for most activities of Nef. Using purified recombinant proteins in pul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alexandra Boeske, Melanie Schwarten, Peixiang Ma, Markus Tusche, Jessica Mötter, Christina Möller, Philipp Neudecker, Silke Hoffmann, Dieter Willbold
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03e0e78ce7bf4b4b9c679247f861c77c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:03e0e78ce7bf4b4b9c679247f861c77c
record_format	dspace
spelling	oai:doaj.org-article:03e0e78ce7bf4b4b9c679247f861c77c2021-12-02T15:05:04ZDirect binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization10.1038/s41598-017-06319-42045-2322https://doaj.org/article/03e0e78ce7bf4b4b9c679247f861c77c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06319-4https://doaj.org/toc/2045-2322Abstract HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis. Studies have shown that the association of Nef with the inner leaflet of the plasma membrane and with endocytic and perinuclear vesicles is essential for most activities of Nef. Using purified recombinant proteins in pull-down assays and by co-immunoprecipitation assays we demonstrate that Nef binds directly and specifically to all GABARAP family members, but not to LC3 family members. Based on nuclear magnetic resonance (NMR) experiments we showed that Nef binds to GABARAP via two surface exposed hydrophobic pockets. S53 and F62 of GABARAP were identified as key residues for the interaction with Nef. During live-cell fluorescence microscopy an accumulation of Nef and all GABARAP family members in vesicular structures throughout the cytoplasm and at the plasma membrane was observed. This plasma membrane accumulation was significantly reduced after knocking down GABARAP, GABARAPL1 and GABARAPL2 with respective siRNAs. We identified GABARAPs as the first known direct interaction partners of Nef that are essential for its plasma membrane localization.Alexandra BoeskeMelanie SchwartenPeixiang MaMarkus TuscheJessica MötterChristina MöllerPhilipp NeudeckerSilke HoffmannDieter WillboldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Boeske Melanie Schwarten Peixiang Ma Markus Tusche Jessica Mötter Christina Möller Philipp Neudecker Silke Hoffmann Dieter Willbold Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
description	Abstract HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis. Studies have shown that the association of Nef with the inner leaflet of the plasma membrane and with endocytic and perinuclear vesicles is essential for most activities of Nef. Using purified recombinant proteins in pull-down assays and by co-immunoprecipitation assays we demonstrate that Nef binds directly and specifically to all GABARAP family members, but not to LC3 family members. Based on nuclear magnetic resonance (NMR) experiments we showed that Nef binds to GABARAP via two surface exposed hydrophobic pockets. S53 and F62 of GABARAP were identified as key residues for the interaction with Nef. During live-cell fluorescence microscopy an accumulation of Nef and all GABARAP family members in vesicular structures throughout the cytoplasm and at the plasma membrane was observed. This plasma membrane accumulation was significantly reduced after knocking down GABARAP, GABARAPL1 and GABARAPL2 with respective siRNAs. We identified GABARAPs as the first known direct interaction partners of Nef that are essential for its plasma membrane localization.
format	article
author	Alexandra Boeske Melanie Schwarten Peixiang Ma Markus Tusche Jessica Mötter Christina Möller Philipp Neudecker Silke Hoffmann Dieter Willbold
author_facet	Alexandra Boeske Melanie Schwarten Peixiang Ma Markus Tusche Jessica Mötter Christina Möller Philipp Neudecker Silke Hoffmann Dieter Willbold
author_sort	Alexandra Boeske
title	Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
title_short	Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
title_full	Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
title_fullStr	Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
title_full_unstemmed	Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
title_sort	direct binding to gabarap family members is essential for hiv-1 nef plasma membrane localization
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/03e0e78ce7bf4b4b9c679247f861c77c
work_keys_str_mv	AT alexandraboeske directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT melanieschwarten directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT peixiangma directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT markustusche directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT jessicamotter directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT christinamoller directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT philippneudecker directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT silkehoffmann directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization AT dieterwillbold directbindingtogabarapfamilymembersisessentialforhiv1nefplasmamembranelocalization
_version_	1718388933706907648

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization

Ejemplares similares