Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes

Abstract Background The two-line method based on the photoperiod and thermo-sensitive genic male sterile (PTGMS) lines is more cost-effective, simple, and efficient than the three-line system based on cytoplasmic male-sterility. Blast and drought are the most prevalent biotic and abiotic stress fact...

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Autores principales:	Yi Liu, Fenyun Zhang, Xingxing Luo, Deyan Kong, Anning Zhang, Feiming Wang, Zhongquan Pan, Jiahong Wang, Junguo Bi, Lijun Luo, Guolan Liu, Xinqiao Yu
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Photoperiod and thermo-sensitive genic male sterile line (PTGMS) Blast resistance Marker-assisted selection Water-saving and drought-resistance rice (WDR) Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/03e7142438b542eaae177b6ce7e601b3
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spelling	oai:doaj.org-article:03e7142438b542eaae177b6ce7e601b32021-11-28T12:38:15ZMolecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes10.1186/s12284-021-00537-11939-84251939-8433https://doaj.org/article/03e7142438b542eaae177b6ce7e601b32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12284-021-00537-1https://doaj.org/toc/1939-8425https://doaj.org/toc/1939-8433Abstract Background The two-line method based on the photoperiod and thermo-sensitive genic male sterile (PTGMS) lines is more cost-effective, simple, and efficient than the three-line system based on cytoplasmic male-sterility. Blast and drought are the most prevalent biotic and abiotic stress factors hampering rice production. Molecular techniques demonstrate higher efficacy in the pyramiding of disease resistance genes, providing green performance under the background of water-saving and drought-resistance rice. Results This study employed molecular marker-assisted selection, conventional hybridization, and high-intensity stress screening to integrate three broad-spectrum blast resistance genes Pi9, Pi5, and Pi54 into Huhan 1S. Subsequently, a novel water-saving and drought-resistance rice (WDR) PTGMS line Huhan 74S was developed. The drought resistance of the new PTGMS line Huhan 74S was comparable to that of Huhan 1S. Pathogenicity assays involving the inoculation of 14 blast prevalent isolates in the glasshouse showed that the blast resistance frequency of Huhan 74S was 85.7%. Further evaluation under natural blast epidemic field conditions showed that Huhan 74S and its hybrids were resistant to leaf and neck blast. The critical temperature point of fertility-sterility alteration of Huhan 74S was 23 °C daily mean temperature. The complete male sterility under natural growth conditions in 2017 at Shanghai lasted for 67 days. Also, both the agronomic and grain quality traits met the requirement for two-line hybrid rice production. Conclusion These results indicate that the newly bred PTGMS line Huhan 74S can be used to breed high-yielding, good-quality, disease-resistant two-line hybrid water-saving and drought-resistance rice (WDR), hence promoting sustainable rice production in China.Yi LiuFenyun ZhangXingxing LuoDeyan KongAnning ZhangFeiming WangZhongquan PanJiahong WangJunguo BiLijun LuoGuolan LiuXinqiao YuSpringerOpenarticlePhotoperiod and thermo-sensitive genic male sterile line (PTGMS)Blast resistanceMarker-assisted selectionWater-saving and drought-resistance rice (WDR)Plant cultureSB1-1110ENRice, Vol 14, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Photoperiod and thermo-sensitive genic male sterile line (PTGMS) Blast resistance Marker-assisted selection Water-saving and drought-resistance rice (WDR) Plant culture SB1-1110
spellingShingle	Photoperiod and thermo-sensitive genic male sterile line (PTGMS) Blast resistance Marker-assisted selection Water-saving and drought-resistance rice (WDR) Plant culture SB1-1110 Yi Liu Fenyun Zhang Xingxing Luo Deyan Kong Anning Zhang Feiming Wang Zhongquan Pan Jiahong Wang Junguo Bi Lijun Luo Guolan Liu Xinqiao Yu Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes
description	Abstract Background The two-line method based on the photoperiod and thermo-sensitive genic male sterile (PTGMS) lines is more cost-effective, simple, and efficient than the three-line system based on cytoplasmic male-sterility. Blast and drought are the most prevalent biotic and abiotic stress factors hampering rice production. Molecular techniques demonstrate higher efficacy in the pyramiding of disease resistance genes, providing green performance under the background of water-saving and drought-resistance rice. Results This study employed molecular marker-assisted selection, conventional hybridization, and high-intensity stress screening to integrate three broad-spectrum blast resistance genes Pi9, Pi5, and Pi54 into Huhan 1S. Subsequently, a novel water-saving and drought-resistance rice (WDR) PTGMS line Huhan 74S was developed. The drought resistance of the new PTGMS line Huhan 74S was comparable to that of Huhan 1S. Pathogenicity assays involving the inoculation of 14 blast prevalent isolates in the glasshouse showed that the blast resistance frequency of Huhan 74S was 85.7%. Further evaluation under natural blast epidemic field conditions showed that Huhan 74S and its hybrids were resistant to leaf and neck blast. The critical temperature point of fertility-sterility alteration of Huhan 74S was 23 °C daily mean temperature. The complete male sterility under natural growth conditions in 2017 at Shanghai lasted for 67 days. Also, both the agronomic and grain quality traits met the requirement for two-line hybrid rice production. Conclusion These results indicate that the newly bred PTGMS line Huhan 74S can be used to breed high-yielding, good-quality, disease-resistant two-line hybrid water-saving and drought-resistance rice (WDR), hence promoting sustainable rice production in China.
format	article
author	Yi Liu Fenyun Zhang Xingxing Luo Deyan Kong Anning Zhang Feiming Wang Zhongquan Pan Jiahong Wang Junguo Bi Lijun Luo Guolan Liu Xinqiao Yu
author_facet	Yi Liu Fenyun Zhang Xingxing Luo Deyan Kong Anning Zhang Feiming Wang Zhongquan Pan Jiahong Wang Junguo Bi Lijun Luo Guolan Liu Xinqiao Yu
author_sort	Yi Liu
title	Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes
title_short	Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes
title_full	Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes
title_fullStr	Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes
title_full_unstemmed	Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes
title_sort	molecular breeding of a novel ptgms line of wdr for broad-spectrum resistance to blast using pi9, pi5, and pi54 genes
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/03e7142438b542eaae177b6ce7e601b3
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Molecular Breeding of a Novel PTGMS Line of WDR for Broad-Spectrum Resistance to Blast Using Pi9, Pi5, and Pi54 Genes

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