Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.

<h4>Purposes</h4>Although clinical and radiological examinations can be used to diagnose oral cancer, and surgical pathology remains the gold standard, these conventional methods have limitations. We evaluated the feasibility of longitudinal next-generation sequencing-based liquid biopsy...

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Autores principales:	Ying Cui, Hae-Suk Kim, Eunae Sandra Cho, Dawool Han, Jung Ah Park, Ju Yeong Park, Woong Nam, Hyung Jun Kim, In-Ho Cha, Yong Hoon Cha
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03e76eaa92f44343a9608c5423686d83
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spelling	oai:doaj.org-article:03e76eaa92f44343a9608c5423686d832021-12-02T20:14:49ZLongitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.1932-620310.1371/journal.pone.0256979https://doaj.org/article/03e76eaa92f44343a9608c5423686d832021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256979https://doaj.org/toc/1932-6203<h4>Purposes</h4>Although clinical and radiological examinations can be used to diagnose oral cancer, and surgical pathology remains the gold standard, these conventional methods have limitations. We evaluated the feasibility of longitudinal next-generation sequencing-based liquid biopsy for oral squamous cell carcinoma surveillance.<h4>Materials and methods</h4>Eleven patients were enrolled, and plasma and saliva were collected before, and 1, 3, and 6 months after surgery. Tumor-specific mutations were selected using paired, whole-exome analyses of tumor tissues and whole blood. Genes frequently mutated in head and neck cancer were identified using the Cancer Genome Atlas (TCGA) and Catalogue of Somatic Mutations in Cancer (COSMIC) databases to design targeted deep sequencing panels.<h4>Results</h4>In five of the six patients with recurrent cancer, circulating tumor DNA (ctDNA) was detected earlier with liquid biopsy than with conventional monitoring techniques. Moreover, patients without recurrence exhibited decreased ctDNA allele frequency post-treatment.<h4>Conclusions</h4>Longitudinal liquid biopsy of plasma and saliva may be feasible for detecting somatic mutations associated with oral squamous cell carcinomas. It might be attributable to determine early tumor recurrence through genetic analysis of ctDNA.Ying CuiHae-Suk KimEunae Sandra ChoDawool HanJung Ah ParkJu Yeong ParkWoong NamHyung Jun KimIn-Ho ChaYong Hoon ChaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256979 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Cui Hae-Suk Kim Eunae Sandra Cho Dawool Han Jung Ah Park Ju Yeong Park Woong Nam Hyung Jun Kim In-Ho Cha Yong Hoon Cha Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
description	<h4>Purposes</h4>Although clinical and radiological examinations can be used to diagnose oral cancer, and surgical pathology remains the gold standard, these conventional methods have limitations. We evaluated the feasibility of longitudinal next-generation sequencing-based liquid biopsy for oral squamous cell carcinoma surveillance.<h4>Materials and methods</h4>Eleven patients were enrolled, and plasma and saliva were collected before, and 1, 3, and 6 months after surgery. Tumor-specific mutations were selected using paired, whole-exome analyses of tumor tissues and whole blood. Genes frequently mutated in head and neck cancer were identified using the Cancer Genome Atlas (TCGA) and Catalogue of Somatic Mutations in Cancer (COSMIC) databases to design targeted deep sequencing panels.<h4>Results</h4>In five of the six patients with recurrent cancer, circulating tumor DNA (ctDNA) was detected earlier with liquid biopsy than with conventional monitoring techniques. Moreover, patients without recurrence exhibited decreased ctDNA allele frequency post-treatment.<h4>Conclusions</h4>Longitudinal liquid biopsy of plasma and saliva may be feasible for detecting somatic mutations associated with oral squamous cell carcinomas. It might be attributable to determine early tumor recurrence through genetic analysis of ctDNA.
format	article
author	Ying Cui Hae-Suk Kim Eunae Sandra Cho Dawool Han Jung Ah Park Ju Yeong Park Woong Nam Hyung Jun Kim In-Ho Cha Yong Hoon Cha
author_facet	Ying Cui Hae-Suk Kim Eunae Sandra Cho Dawool Han Jung Ah Park Ju Yeong Park Woong Nam Hyung Jun Kim In-Ho Cha Yong Hoon Cha
author_sort	Ying Cui
title	Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
title_short	Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
title_full	Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
title_fullStr	Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
title_full_unstemmed	Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
title_sort	longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/03e76eaa92f44343a9608c5423686d83
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Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.

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