Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology

Hypertension, a common chronic condition, may damage multiple organs, including the kidney, heart, and brain. Thus, it is essential to understand the pathology upon ectopic activation of the molecular pathways involved in mammalian hypertension to develop strategies to manage hypertension. Animal mo...

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Autores principales:	Bhagyashri Joshi, Ganesh Wagh, Harmandeep Kaur, Chinmoy Patra
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hypertension AngII zebrafish cardiac remodeling fibrosis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/03ebf1a49f9b4a7b89966aa063b3aaa8
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spelling	oai:doaj.org-article:03ebf1a49f9b4a7b89966aa063b3aaa82021-11-25T16:47:43ZZebrafish Model to Study Angiotensin II-Mediated Pathophysiology10.3390/biology101111772079-7737https://doaj.org/article/03ebf1a49f9b4a7b89966aa063b3aaa82021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1177https://doaj.org/toc/2079-7737Hypertension, a common chronic condition, may damage multiple organs, including the kidney, heart, and brain. Thus, it is essential to understand the pathology upon ectopic activation of the molecular pathways involved in mammalian hypertension to develop strategies to manage hypertension. Animal models play a crucial role in unraveling the disease pathophysiology by allowing incisive experimental procedures impossible in humans. Zebrafish, a small freshwater fish, have emerged as an important model system to study human diseases. The primary effector, Angiotensin II of the RAS pathway, regulates hemodynamic pressure overload mediated cardiovascular pathogenesis in mammals. There are various established mammalian models available to study pathophysiology in Angiotensin II-induced hypertension. Here, we have developed a zebrafish model to study pathogenesis by Angiotensin II. We find that intradermal Angiotensin II injection every 12 h can induce cardiac remodeling in seven days. We show that Angiotensin II injection in adult zebrafish causes cardiomyocyte hypertrophy and enhances cardiac cell proliferation. In addition, Angiotensin II induces ECM protein-coding gene expression and fibrosis in the cardiac ventricles. Thus, this study can conclude that Angiotensin II injection in zebrafish has similar implications as mammals, and zebrafish can be a model to study pathophysiology associated with AngII-RAS signaling.Bhagyashri JoshiGanesh WaghHarmandeep KaurChinmoy PatraMDPI AGarticlehypertensionAngIIzebrafishcardiac remodelingfibrosisBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1177, p 1177 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypertension AngII zebrafish cardiac remodeling fibrosis Biology (General) QH301-705.5
spellingShingle	hypertension AngII zebrafish cardiac remodeling fibrosis Biology (General) QH301-705.5 Bhagyashri Joshi Ganesh Wagh Harmandeep Kaur Chinmoy Patra Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology
description	Hypertension, a common chronic condition, may damage multiple organs, including the kidney, heart, and brain. Thus, it is essential to understand the pathology upon ectopic activation of the molecular pathways involved in mammalian hypertension to develop strategies to manage hypertension. Animal models play a crucial role in unraveling the disease pathophysiology by allowing incisive experimental procedures impossible in humans. Zebrafish, a small freshwater fish, have emerged as an important model system to study human diseases. The primary effector, Angiotensin II of the RAS pathway, regulates hemodynamic pressure overload mediated cardiovascular pathogenesis in mammals. There are various established mammalian models available to study pathophysiology in Angiotensin II-induced hypertension. Here, we have developed a zebrafish model to study pathogenesis by Angiotensin II. We find that intradermal Angiotensin II injection every 12 h can induce cardiac remodeling in seven days. We show that Angiotensin II injection in adult zebrafish causes cardiomyocyte hypertrophy and enhances cardiac cell proliferation. In addition, Angiotensin II induces ECM protein-coding gene expression and fibrosis in the cardiac ventricles. Thus, this study can conclude that Angiotensin II injection in zebrafish has similar implications as mammals, and zebrafish can be a model to study pathophysiology associated with AngII-RAS signaling.
format	article
author	Bhagyashri Joshi Ganesh Wagh Harmandeep Kaur Chinmoy Patra
author_facet	Bhagyashri Joshi Ganesh Wagh Harmandeep Kaur Chinmoy Patra
author_sort	Bhagyashri Joshi
title	Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology
title_short	Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology
title_full	Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology
title_fullStr	Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology
title_full_unstemmed	Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology
title_sort	zebrafish model to study angiotensin ii-mediated pathophysiology
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/03ebf1a49f9b4a7b89966aa063b3aaa8
work_keys_str_mv	AT bhagyashrijoshi zebrafishmodeltostudyangiotensiniimediatedpathophysiology AT ganeshwagh zebrafishmodeltostudyangiotensiniimediatedpathophysiology AT harmandeepkaur zebrafishmodeltostudyangiotensiniimediatedpathophysiology AT chinmoypatra zebrafishmodeltostudyangiotensiniimediatedpathophysiology
_version_	1718412936857255936

Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology

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