Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
<i>Stryphnodendron rotundifolium</i> Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study inves...
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| Autores principales: | , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/03ecafbb55d24974916edd7199646a60 |
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| Sumario: | <i>Stryphnodendron rotundifolium</i> Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study investigated the protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds were quantified and identified by Ultra-Performance Liquid Chromatography coupled with quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (<i>UPLC–ESI-qTOF-MS/MS</i>) in positive and negative ions mode analysis. Antioxidant activity was analyzed following the iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents, prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly protected 2-DR degradation induced by Fe<sup>2+</sup> (72% protection) or <sup>•</sup>OH (43% protection). The <i>ortho</i>-phenanthroline test revealed Fe<sup>2+</sup>-chelating and Fe<sup>3+</sup>-reducing activities of 93% and 84%, respectively. A preliminary toxicological analysis using <i>Artemia salina</i> revealed mortality below 10%, at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions. In conclusion, the findings of the present study indicate that <i>Stryphnodendron rotundifolium</i> is a source of antioxidant compounds with the potential to be used in drug development in the context of iron overload disorders, which remains to be further investigated in vivo. |
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