Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model

<i>Stryphnodendron rotundifolium</i> Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study inves...

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Autores principales:	Gerson Javier Torres Salazar, Francisco Junio Dias, Paulo Riceli Vasconcelos Ribeiro, Edy Sousa de Brito, Kirley Marques Canuto, Henrique Douglas Melo Coutinho, Jaime Ribeiro-Filho, Monica Gallo, Domenico Montesano, Daniele Naviglio, Gokhan Zengin, José Galberto Martins da Costa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidant activity free radicals iron overload <i>Stryphnodendron rotundifolium</i> tannins <i>UPLC–ESI-qTOF-MS/MS</i> Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/03ecafbb55d24974916edd7199646a60
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spelling	oai:doaj.org-article:03ecafbb55d24974916edd7199646a602021-11-25T17:34:06ZAntioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model10.3390/foods101126832304-8158https://doaj.org/article/03ecafbb55d24974916edd7199646a602021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2683https://doaj.org/toc/2304-8158<i>Stryphnodendron rotundifolium</i> Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study investigated the protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds were quantified and identified by Ultra-Performance Liquid Chromatography coupled with quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (<i>UPLC–ESI-qTOF-MS/MS</i>) in positive and negative ions mode analysis. Antioxidant activity was analyzed following the iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents, prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly protected 2-DR degradation induced by Fe<sup>2+</sup> (72% protection) or <sup>•</sup>OH (43% protection). The <i>ortho</i>-phenanthroline test revealed Fe<sup>2+</sup>-chelating and Fe<sup>3+</sup>-reducing activities of 93% and 84%, respectively. A preliminary toxicological analysis using <i>Artemia salina</i> revealed mortality below 10%, at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions. In conclusion, the findings of the present study indicate that <i>Stryphnodendron rotundifolium</i> is a source of antioxidant compounds with the potential to be used in drug development in the context of iron overload disorders, which remains to be further investigated in vivo.Gerson Javier Torres SalazarFrancisco Junio DiasPaulo Riceli Vasconcelos RibeiroEdy Sousa de BritoKirley Marques CanutoHenrique Douglas Melo CoutinhoJaime Ribeiro-FilhoMonica GalloDomenico MontesanoDaniele NaviglioGokhan ZenginJosé Galberto Martins da CostaMDPI AGarticleantioxidant activityfree radicalsiron overload<i>Stryphnodendron rotundifolium</i>tannins<i>UPLC–ESI-qTOF-MS/MS</i>Chemical technologyTP1-1185ENFoods, Vol 10, Iss 2683, p 2683 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant activity free radicals iron overload <i>Stryphnodendron rotundifolium</i> tannins <i>UPLC–ESI-qTOF-MS/MS</i> Chemical technology TP1-1185
spellingShingle	antioxidant activity free radicals iron overload <i>Stryphnodendron rotundifolium</i> tannins <i>UPLC–ESI-qTOF-MS/MS</i> Chemical technology TP1-1185 Gerson Javier Torres Salazar Francisco Junio Dias Paulo Riceli Vasconcelos Ribeiro Edy Sousa de Brito Kirley Marques Canuto Henrique Douglas Melo Coutinho Jaime Ribeiro-Filho Monica Gallo Domenico Montesano Daniele Naviglio Gokhan Zengin José Galberto Martins da Costa Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
description	<i>Stryphnodendron rotundifolium</i> Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study investigated the protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds were quantified and identified by Ultra-Performance Liquid Chromatography coupled with quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (<i>UPLC–ESI-qTOF-MS/MS</i>) in positive and negative ions mode analysis. Antioxidant activity was analyzed following the iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents, prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly protected 2-DR degradation induced by Fe<sup>2+</sup> (72% protection) or <sup>•</sup>OH (43% protection). The <i>ortho</i>-phenanthroline test revealed Fe<sup>2+</sup>-chelating and Fe<sup>3+</sup>-reducing activities of 93% and 84%, respectively. A preliminary toxicological analysis using <i>Artemia salina</i> revealed mortality below 10%, at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions. In conclusion, the findings of the present study indicate that <i>Stryphnodendron rotundifolium</i> is a source of antioxidant compounds with the potential to be used in drug development in the context of iron overload disorders, which remains to be further investigated in vivo.
format	article
author	Gerson Javier Torres Salazar Francisco Junio Dias Paulo Riceli Vasconcelos Ribeiro Edy Sousa de Brito Kirley Marques Canuto Henrique Douglas Melo Coutinho Jaime Ribeiro-Filho Monica Gallo Domenico Montesano Daniele Naviglio Gokhan Zengin José Galberto Martins da Costa
author_facet	Gerson Javier Torres Salazar Francisco Junio Dias Paulo Riceli Vasconcelos Ribeiro Edy Sousa de Brito Kirley Marques Canuto Henrique Douglas Melo Coutinho Jaime Ribeiro-Filho Monica Gallo Domenico Montesano Daniele Naviglio Gokhan Zengin José Galberto Martins da Costa
author_sort	Gerson Javier Torres Salazar
title	Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
title_short	Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
title_full	Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
title_fullStr	Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
title_full_unstemmed	Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model
title_sort	antioxidant activity of <i>stryphnodendron rotundifolium</i> mart. stem bark fraction in an iron overload model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/03ecafbb55d24974916edd7199646a60
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Antioxidant Activity of <i>Stryphnodendron rotundifolium</i> Mart. Stem Bark Fraction in an Iron Overload Model

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