In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media

Abstract. Hapsoro D, Hamiranti R, Yusnita Y. 2020. In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media. Biodiversitas 21: 3811-3817. This study aimed to investigate the effects of genotypes and primary callus ind...

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Autores principales:	dwi hapsoro, rahmadyah hamiranti, yusnita yusnita
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	2.4 d, coffea canephora, culture, regeneration, thidiazuron Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/03ee4d393120449c9019a5938ffe76ae
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spelling	oai:doaj.org-article:03ee4d393120449c9019a5938ffe76ae2021-11-22T12:17:57ZIn vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media1412-033X2085-472210.13057/biodiv/d210849https://doaj.org/article/03ee4d393120449c9019a5938ffe76ae2020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5945https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Hapsoro D, Hamiranti R, Yusnita Y. 2020. In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media. Biodiversitas 21: 3811-3817. This study aimed to investigate the effects of genotypes and primary callus induction media on somatic embryogenesis of superior robusta coffee clones of Lampung. Leaf explants of clones Tugusari, Komari, Tugino, and Wanto were cultured on two types of primary callus induction media (PCIM). PCIM1 consisted of half-strength MS salts, 30 gL-1 sucrose, added with (mgL-1) 0.1 thiamine-HCl, 0.5 nicotinic acids, 0.5 pyridoxine-HCl, 100 Myo-inositol, 200 ascorbic acids, 150 citric acids, and 1 benzyl adenine. PCIM2 consisted of NPCM salts, 30 gL-1 sucrose, added with (mgL-1) 15 thiamine-HCl, 1 nicotinic acid, 1 pyridoxine-HCl, 2 glycines, 130 Myo-inositol, 200 ascorbic acids, 150 citric acids, 1 2,4-dichlorophenoxyacetic acid, and 2 thidiazuron. The highest percentage (100%) of primary callus formation was found in Komari and Wanto clones. PCIM2 resulted in more primary calli than PCIM1. When subcultured to embryogenic callus induction medium, primary calli of clone Komari and Wanto developed into a high percentage of embryogenic calli, while those of the other two turned brown and died. PCIM2-derived primary calli developed into more embryogenic calli. When subcultured on somatic embryo (SE) regeneration medium, these calli underwent the formation of SE of various stages. When subcultured to plant regeneration medium, these SEs developed into plantlets.dwi hapsororahmadyah hamirantiyusnita yusnitaMBI & UNS Soloarticle2.4 d, coffea canephora, culture, regeneration, thidiazuronBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	2.4 d, coffea canephora, culture, regeneration, thidiazuron Biology (General) QH301-705.5
spellingShingle	2.4 d, coffea canephora, culture, regeneration, thidiazuron Biology (General) QH301-705.5 dwi hapsoro rahmadyah hamiranti yusnita yusnita In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media
description	Abstract. Hapsoro D, Hamiranti R, Yusnita Y. 2020. In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media. Biodiversitas 21: 3811-3817. This study aimed to investigate the effects of genotypes and primary callus induction media on somatic embryogenesis of superior robusta coffee clones of Lampung. Leaf explants of clones Tugusari, Komari, Tugino, and Wanto were cultured on two types of primary callus induction media (PCIM). PCIM1 consisted of half-strength MS salts, 30 gL-1 sucrose, added with (mgL-1) 0.1 thiamine-HCl, 0.5 nicotinic acids, 0.5 pyridoxine-HCl, 100 Myo-inositol, 200 ascorbic acids, 150 citric acids, and 1 benzyl adenine. PCIM2 consisted of NPCM salts, 30 gL-1 sucrose, added with (mgL-1) 15 thiamine-HCl, 1 nicotinic acid, 1 pyridoxine-HCl, 2 glycines, 130 Myo-inositol, 200 ascorbic acids, 150 citric acids, 1 2,4-dichlorophenoxyacetic acid, and 2 thidiazuron. The highest percentage (100%) of primary callus formation was found in Komari and Wanto clones. PCIM2 resulted in more primary calli than PCIM1. When subcultured to embryogenic callus induction medium, primary calli of clone Komari and Wanto developed into a high percentage of embryogenic calli, while those of the other two turned brown and died. PCIM2-derived primary calli developed into more embryogenic calli. When subcultured on somatic embryo (SE) regeneration medium, these calli underwent the formation of SE of various stages. When subcultured to plant regeneration medium, these SEs developed into plantlets.
format	article
author	dwi hapsoro rahmadyah hamiranti yusnita yusnita
author_facet	dwi hapsoro rahmadyah hamiranti yusnita yusnita
author_sort	dwi hapsoro
title	In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media
title_short	In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media
title_full	In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media
title_fullStr	In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media
title_full_unstemmed	In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media
title_sort	in vitro somatic embryogenesis of superior clones of robusta coffee from lampung, indonesia: effect of genotypes and callus induction media
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/03ee4d393120449c9019a5938ffe76ae
work_keys_str_mv	AT dwihapsoro invitrosomaticembryogenesisofsuperiorclonesofrobustacoffeefromlampungindonesiaeffectofgenotypesandcallusinductionmedia AT rahmadyahhamiranti invitrosomaticembryogenesisofsuperiorclonesofrobustacoffeefromlampungindonesiaeffectofgenotypesandcallusinductionmedia AT yusnitayusnita invitrosomaticembryogenesisofsuperiorclonesofrobustacoffeefromlampungindonesiaeffectofgenotypesandcallusinductionmedia
_version_	1718417655164043264

In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media

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