Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes

Catalase is known for its ability to catalyze disproportionation of hydrogen peroxide into oxygen and water. This work reports the oxygen reduction reaction (ORR) catalyzed by catalase, with a decreased activation energy manifested by a positive shift (+0.2 V) in onset potential. Direct bioelectroca...

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Autores principales:	Agata Roguska, Adam Leśniewski, Marcin Opallo, Wojciech Nogala
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Direct biocatalysis Enzyme Mercury–gold amalgam Hemispherical microelectrode Cyclic voltammetry ORR Industrial electrochemistry TP250-261 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/03fda2e04c7b4e318bac9837ba00cb21
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id	oai:doaj.org-article:03fda2e04c7b4e318bac9837ba00cb21
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spelling	oai:doaj.org-article:03fda2e04c7b4e318bac9837ba00cb212021-12-04T04:33:23ZMediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes1388-248110.1016/j.elecom.2021.107167https://doaj.org/article/03fda2e04c7b4e318bac9837ba00cb212021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1388248121002514https://doaj.org/toc/1388-2481Catalase is known for its ability to catalyze disproportionation of hydrogen peroxide into oxygen and water. This work reports the oxygen reduction reaction (ORR) catalyzed by catalase, with a decreased activation energy manifested by a positive shift (+0.2 V) in onset potential. Direct bioelectrocatalysis with bovine liver catalase on a mercury–gold amalgam microelectrode is demonstrated. The background oxygen reduction reaction on the amalgam surface is inhibited by the protein, while the hydrogen evolution reaction (HER) is facilitated. Catalase molecules whose prosthetic groups are not wired to the electrode retain their intrinsic activity towards disproportionation of H2O2 generated at the electrode. The Hg–Au amalgam electrode may also be used to detect dissolved catalase in the absence of its substrate (H2O2) in the solution.Agata RoguskaAdam LeśniewskiMarcin OpalloWojciech NogalaElsevierarticleDirect biocatalysisEnzymeMercury–gold amalgamHemispherical microelectrodeCyclic voltammetryORRIndustrial electrochemistryTP250-261ChemistryQD1-999ENElectrochemistry Communications, Vol 133, Iss , Pp 107167- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Direct biocatalysis Enzyme Mercury–gold amalgam Hemispherical microelectrode Cyclic voltammetry ORR Industrial electrochemistry TP250-261 Chemistry QD1-999
spellingShingle	Direct biocatalysis Enzyme Mercury–gold amalgam Hemispherical microelectrode Cyclic voltammetry ORR Industrial electrochemistry TP250-261 Chemistry QD1-999 Agata Roguska Adam Leśniewski Marcin Opallo Wojciech Nogala Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
description	Catalase is known for its ability to catalyze disproportionation of hydrogen peroxide into oxygen and water. This work reports the oxygen reduction reaction (ORR) catalyzed by catalase, with a decreased activation energy manifested by a positive shift (+0.2 V) in onset potential. Direct bioelectrocatalysis with bovine liver catalase on a mercury–gold amalgam microelectrode is demonstrated. The background oxygen reduction reaction on the amalgam surface is inhibited by the protein, while the hydrogen evolution reaction (HER) is facilitated. Catalase molecules whose prosthetic groups are not wired to the electrode retain their intrinsic activity towards disproportionation of H2O2 generated at the electrode. The Hg–Au amalgam electrode may also be used to detect dissolved catalase in the absence of its substrate (H2O2) in the solution.
format	article
author	Agata Roguska Adam Leśniewski Marcin Opallo Wojciech Nogala
author_facet	Agata Roguska Adam Leśniewski Marcin Opallo Wojciech Nogala
author_sort	Agata Roguska
title	Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
title_short	Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
title_full	Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
title_fullStr	Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
title_full_unstemmed	Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
title_sort	mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/03fda2e04c7b4e318bac9837ba00cb21
work_keys_str_mv	AT agataroguska mediatorlesselectrocatalyticoxygenreductionwithcatalaseonmercurygoldamalgammicroelectrodes AT adamlesniewski mediatorlesselectrocatalyticoxygenreductionwithcatalaseonmercurygoldamalgammicroelectrodes AT marcinopallo mediatorlesselectrocatalyticoxygenreductionwithcatalaseonmercurygoldamalgammicroelectrodes AT wojciechnogala mediatorlesselectrocatalyticoxygenreductionwithcatalaseonmercurygoldamalgammicroelectrodes
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Mediatorless electrocatalytic oxygen reduction with catalase on mercury–gold amalgam microelectrodes

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