Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018

Abstract Rapid diagnostic tests (RDTs) detecting histidine-rich protein 2 (HRP2) and HRP3 are widely used throughout sub-Saharan Africa (SSA) to diagnose Plasmodium falciparum malaria. However, multiple SSA countries have reported pfhrp2 and pfhrp3 (pfhrp2/3) gene deletions. Blood samples (n = 1109)...

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Autores principales:	Jessica N. McCaffery, Douglas Nace, Camelia Herman, Balwan Singh, Eric Mukomena Sompwe, Papy Mandoko Nkoli, Dieudonné Mumba Ngoyi, Gauthier Mesia Kahunu, Eric S. Halsey, Eric Rogier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03ffc4d8850543d28ee7154f6741d18d
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spelling	oai:doaj.org-article:03ffc4d8850543d28ee7154f6741d18d2021-11-28T12:21:28ZPlasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 201810.1038/s41598-021-02452-32045-2322https://doaj.org/article/03ffc4d8850543d28ee7154f6741d18d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02452-3https://doaj.org/toc/2045-2322Abstract Rapid diagnostic tests (RDTs) detecting histidine-rich protein 2 (HRP2) and HRP3 are widely used throughout sub-Saharan Africa (SSA) to diagnose Plasmodium falciparum malaria. However, multiple SSA countries have reported pfhrp2 and pfhrp3 (pfhrp2/3) gene deletions. Blood samples (n = 1109) collected from patients with P. falciparum infection from six health facilities throughout the Democratic Republic of the Congo (DRC) from March 2017 to January 2018 were evaluated for pfhrp2/3 deletions. Samples were assayed for HRP2, pan-Plasmodium LDH (pLDH) and aldolase (pAldolase) antigens by bead-based multiplex antigen assay. Samples with low HRP2 concentration compared to pLDH and pAldolase antigens were selected for further pfhrp2/3 genotyping PCRs. The majority of blood samples (93.3%, 1035/1109) had high concentrations of the HRP2 antigen. Single deletions of pfhrp2 were identified in 0.27% (3/1109) of screened samples, with one sample from each of the Kapolowe, Mikalayi, and Rutshuru study sites. A pfhrp3 single deletion (0.09%, 1/1109) was found in the Kapolowe site. Dual pfhrp2 and pfhrp3 deletions were not observed. Due to, the low numbers of pfhrp2 deletions and the sporadic locations of these deletions, the use of HRP2-based RDTs appears to still be appropriate for these locations in DRC.Jessica N. McCafferyDouglas NaceCamelia HermanBalwan SinghEric Mukomena SompwePapy Mandoko NkoliDieudonné Mumba NgoyiGauthier Mesia KahunuEric S. HalseyEric RogierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica N. McCaffery Douglas Nace Camelia Herman Balwan Singh Eric Mukomena Sompwe Papy Mandoko Nkoli Dieudonné Mumba Ngoyi Gauthier Mesia Kahunu Eric S. Halsey Eric Rogier Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018
description	Abstract Rapid diagnostic tests (RDTs) detecting histidine-rich protein 2 (HRP2) and HRP3 are widely used throughout sub-Saharan Africa (SSA) to diagnose Plasmodium falciparum malaria. However, multiple SSA countries have reported pfhrp2 and pfhrp3 (pfhrp2/3) gene deletions. Blood samples (n = 1109) collected from patients with P. falciparum infection from six health facilities throughout the Democratic Republic of the Congo (DRC) from March 2017 to January 2018 were evaluated for pfhrp2/3 deletions. Samples were assayed for HRP2, pan-Plasmodium LDH (pLDH) and aldolase (pAldolase) antigens by bead-based multiplex antigen assay. Samples with low HRP2 concentration compared to pLDH and pAldolase antigens were selected for further pfhrp2/3 genotyping PCRs. The majority of blood samples (93.3%, 1035/1109) had high concentrations of the HRP2 antigen. Single deletions of pfhrp2 were identified in 0.27% (3/1109) of screened samples, with one sample from each of the Kapolowe, Mikalayi, and Rutshuru study sites. A pfhrp3 single deletion (0.09%, 1/1109) was found in the Kapolowe site. Dual pfhrp2 and pfhrp3 deletions were not observed. Due to, the low numbers of pfhrp2 deletions and the sporadic locations of these deletions, the use of HRP2-based RDTs appears to still be appropriate for these locations in DRC.
format	article
author	Jessica N. McCaffery Douglas Nace Camelia Herman Balwan Singh Eric Mukomena Sompwe Papy Mandoko Nkoli Dieudonné Mumba Ngoyi Gauthier Mesia Kahunu Eric S. Halsey Eric Rogier
author_facet	Jessica N. McCaffery Douglas Nace Camelia Herman Balwan Singh Eric Mukomena Sompwe Papy Mandoko Nkoli Dieudonné Mumba Ngoyi Gauthier Mesia Kahunu Eric S. Halsey Eric Rogier
author_sort	Jessica N. McCaffery
title	Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018
title_short	Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018
title_full	Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018
title_fullStr	Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018
title_full_unstemmed	Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018
title_sort	plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the drc enrolled from 2017 to 2018
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/03ffc4d8850543d28ee7154f6741d18d
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Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018

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