Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales

The present study evaluated the physicochemical characterization and cytotoxicity activity of chitosan and chito-oligosaccharides (COSs). The extraction of chitosan and COSs was executed by chemical hydrolysis. The physicochemical characterization and deacetylation (DA) value were determined using a...

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Autores principales:	Gul-e-Saba Chaudhry, C S Thirukanthan, K Murni NurIslamiah, Y Y Sung, T S. M. Sifzizul, A W. M. Effendy
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	biodegradable polymer cancer chito-oligosaccharides chitosan cytotoxicity mts Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/04048e190bc64ab5ba6f595195e0f496
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spelling	oai:doaj.org-article:04048e190bc64ab5ba6f595195e0f4962021-11-12T10:17:53ZCharacterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales2231-40400976-209410.4103/japtr.japtr_117_21https://doaj.org/article/04048e190bc64ab5ba6f595195e0f4962021-01-01T00:00:00Zhttp://www.japtr.org/article.asp?issn=2231-4040;year=2021;volume=12;issue=4;spage=373;epage=377;aulast=Chaudhryhttps://doaj.org/toc/2231-4040https://doaj.org/toc/0976-2094The present study evaluated the physicochemical characterization and cytotoxicity activity of chitosan and chito-oligosaccharides (COSs). The extraction of chitosan and COSs was executed by chemical hydrolysis. The physicochemical characterization and deacetylation (DA) value were determined using an FTIR. The molecular weight was determined by using the Mark–Houwink equation. The physical parameters such as solubility, water-binding capacity (WBC), and fat-binding capacity (FBC) were determination as per equation (i), (ii), and (iii) respectively. The cytotoxic activities of chitosan and COS against MCF-7, HepG2, HeLa-6, and 3T3 were performed by MTS assay. The COS induced enhance cytotoxicity with IC50 0.87 and 2.21 mg/ml against MCF-7 and HepG2 respectively. However, COSs seem to be more sensitive toward the cell lines with the relative potential of MCF-7 > HepG2 > HeLa. Hence, the results showed promising future perspectives of chitosan and COS to develop biodegradable, antibacterial, cytotoxic naturally derived polysaccharides for cancer drug delivery and smart wound dressings.Gul-e-Saba ChaudhryC S ThirukanthanK Murni NurIslamiahY Y SungT S. M. SifzizulA W. M. EffendyWolters Kluwer Medknow Publicationsarticlebiodegradable polymercancerchito-oligosaccharideschitosancytotoxicitymtsTherapeutics. PharmacologyRM1-950Pharmacy and materia medicaRS1-441ENJournal of Advanced Pharmaceutical Technology & Research, Vol 12, Iss 4, Pp 373-377 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodegradable polymer cancer chito-oligosaccharides chitosan cytotoxicity mts Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
spellingShingle	biodegradable polymer cancer chito-oligosaccharides chitosan cytotoxicity mts Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441 Gul-e-Saba Chaudhry C S Thirukanthan K Murni NurIslamiah Y Y Sung T S. M. Sifzizul A W. M. Effendy Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
description	The present study evaluated the physicochemical characterization and cytotoxicity activity of chitosan and chito-oligosaccharides (COSs). The extraction of chitosan and COSs was executed by chemical hydrolysis. The physicochemical characterization and deacetylation (DA) value were determined using an FTIR. The molecular weight was determined by using the Mark–Houwink equation. The physical parameters such as solubility, water-binding capacity (WBC), and fat-binding capacity (FBC) were determination as per equation (i), (ii), and (iii) respectively. The cytotoxic activities of chitosan and COS against MCF-7, HepG2, HeLa-6, and 3T3 were performed by MTS assay. The COS induced enhance cytotoxicity with IC50 0.87 and 2.21 mg/ml against MCF-7 and HepG2 respectively. However, COSs seem to be more sensitive toward the cell lines with the relative potential of MCF-7 > HepG2 > HeLa. Hence, the results showed promising future perspectives of chitosan and COS to develop biodegradable, antibacterial, cytotoxic naturally derived polysaccharides for cancer drug delivery and smart wound dressings.
format	article
author	Gul-e-Saba Chaudhry C S Thirukanthan K Murni NurIslamiah Y Y Sung T S. M. Sifzizul A W. M. Effendy
author_facet	Gul-e-Saba Chaudhry C S Thirukanthan K Murni NurIslamiah Y Y Sung T S. M. Sifzizul A W. M. Effendy
author_sort	Gul-e-Saba Chaudhry
title	Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
title_short	Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
title_full	Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
title_fullStr	Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
title_full_unstemmed	Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
title_sort	characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/04048e190bc64ab5ba6f595195e0f496
work_keys_str_mv	AT gulesabachaudhry characterizationandcytotoxicityoflowmolecularweightchitosanandchitooligosaccharidesderivedfromtilapiafishscales AT csthirukanthan characterizationandcytotoxicityoflowmolecularweightchitosanandchitooligosaccharidesderivedfromtilapiafishscales AT kmurninurislamiah characterizationandcytotoxicityoflowmolecularweightchitosanandchitooligosaccharidesderivedfromtilapiafishscales AT yysung characterizationandcytotoxicityoflowmolecularweightchitosanandchitooligosaccharidesderivedfromtilapiafishscales AT tsmsifzizul characterizationandcytotoxicityoflowmolecularweightchitosanandchitooligosaccharidesderivedfromtilapiafishscales AT awmeffendy characterizationandcytotoxicityoflowmolecularweightchitosanandchitooligosaccharidesderivedfromtilapiafishscales
_version_	1718430970181320704

Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales

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