On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring
Biomanufacturers are being incited by regulatory agencies to transition from a quality by testing framework, where they extensively test their product after their production, to more of a quality by design or even quality by control framework. This requires powerful analytical tools and sensors enab...
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MDPI AG
2021
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oai:doaj.org-article:0408d0fab6cd45e9a6d1f3b7eea32b4d2021-11-25T18:51:20ZOn the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring10.3390/pr91119962227-9717https://doaj.org/article/0408d0fab6cd45e9a6d1f3b7eea32b4d2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1996https://doaj.org/toc/2227-9717Biomanufacturers are being incited by regulatory agencies to transition from a quality by testing framework, where they extensively test their product after their production, to more of a quality by design or even quality by control framework. This requires powerful analytical tools and sensors enabling measurements of key process variables and/or product quality attributes during production, preferably in an online manner. As such, the demand for monitoring technologies is rapidly growing. In this context, we believe surface plasmon resonance (SPR)-based biosensors can play a role in enabling the development of improved bioprocess monitoring and control strategies. The SPR technique has been profusely used to probe the binding behavior of a solution species with a sensor surface-immobilized partner in an investigative context, but its ability to detect binding in real-time and without a label has been exploited for monitoring purposes and is promising for the near future. In this review, we examine applications of SPR that are or could be related to bioprocess monitoring in three spheres: biotherapeutics production monitoring, vaccine monitoring, and bacteria and contaminant detection. These applications mainly exploit SPR’s ability to measure solution species concentrations, but performing kinetic analyses is also possible and could prove useful for product quality assessments. We follow with a discussion on the limitations of SPR in a monitoring role and how recent advances in hardware and SPR response modeling could counter them. Mainly, throughput limitations can be addressed by multi-detection spot instruments, and nonspecific binding effects can be alleviated by new antifouling materials. A plethora of methods are available for cell growth and metabolism monitoring, but product monitoring is performed mainly a posteriori. SPR-based biosensors exhibit potential as product monitoring tools from early production to the end of downstream processing, paving the way for more efficient production control. However, more work needs to be done to facilitate or eliminate the need for sample preprocessing and to optimize the experimental protocols.Jimmy GaudreaultCatherine Forest-NaultGregory De CrescenzoYves DurocherOlivier HenryMDPI AGarticlesurface plasmon resonance (SPR)bioprocessmonitoringbiosensorquality by design (QbD)process analytical technology (PAT)Chemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1996, p 1996 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
surface plasmon resonance (SPR) bioprocess monitoring biosensor quality by design (QbD) process analytical technology (PAT) Chemical technology TP1-1185 Chemistry QD1-999 |
| spellingShingle |
surface plasmon resonance (SPR) bioprocess monitoring biosensor quality by design (QbD) process analytical technology (PAT) Chemical technology TP1-1185 Chemistry QD1-999 Jimmy Gaudreault Catherine Forest-Nault Gregory De Crescenzo Yves Durocher Olivier Henry On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring |
| description |
Biomanufacturers are being incited by regulatory agencies to transition from a quality by testing framework, where they extensively test their product after their production, to more of a quality by design or even quality by control framework. This requires powerful analytical tools and sensors enabling measurements of key process variables and/or product quality attributes during production, preferably in an online manner. As such, the demand for monitoring technologies is rapidly growing. In this context, we believe surface plasmon resonance (SPR)-based biosensors can play a role in enabling the development of improved bioprocess monitoring and control strategies. The SPR technique has been profusely used to probe the binding behavior of a solution species with a sensor surface-immobilized partner in an investigative context, but its ability to detect binding in real-time and without a label has been exploited for monitoring purposes and is promising for the near future. In this review, we examine applications of SPR that are or could be related to bioprocess monitoring in three spheres: biotherapeutics production monitoring, vaccine monitoring, and bacteria and contaminant detection. These applications mainly exploit SPR’s ability to measure solution species concentrations, but performing kinetic analyses is also possible and could prove useful for product quality assessments. We follow with a discussion on the limitations of SPR in a monitoring role and how recent advances in hardware and SPR response modeling could counter them. Mainly, throughput limitations can be addressed by multi-detection spot instruments, and nonspecific binding effects can be alleviated by new antifouling materials. A plethora of methods are available for cell growth and metabolism monitoring, but product monitoring is performed mainly a posteriori. SPR-based biosensors exhibit potential as product monitoring tools from early production to the end of downstream processing, paving the way for more efficient production control. However, more work needs to be done to facilitate or eliminate the need for sample preprocessing and to optimize the experimental protocols. |
| format |
article |
| author |
Jimmy Gaudreault Catherine Forest-Nault Gregory De Crescenzo Yves Durocher Olivier Henry |
| author_facet |
Jimmy Gaudreault Catherine Forest-Nault Gregory De Crescenzo Yves Durocher Olivier Henry |
| author_sort |
Jimmy Gaudreault |
| title |
On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring |
| title_short |
On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring |
| title_full |
On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring |
| title_fullStr |
On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring |
| title_full_unstemmed |
On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring |
| title_sort |
on the use of surface plasmon resonance-based biosensors for advanced bioprocess monitoring |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/0408d0fab6cd45e9a6d1f3b7eea32b4d |
| work_keys_str_mv |
AT jimmygaudreault ontheuseofsurfaceplasmonresonancebasedbiosensorsforadvancedbioprocessmonitoring AT catherineforestnault ontheuseofsurfaceplasmonresonancebasedbiosensorsforadvancedbioprocessmonitoring AT gregorydecrescenzo ontheuseofsurfaceplasmonresonancebasedbiosensorsforadvancedbioprocessmonitoring AT yvesdurocher ontheuseofsurfaceplasmonresonancebasedbiosensorsforadvancedbioprocessmonitoring AT olivierhenry ontheuseofsurfaceplasmonresonancebasedbiosensorsforadvancedbioprocessmonitoring |
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