Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon.
The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25 °C, whereas Bd21 is susceptible and infection results in an...
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2012
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oai:doaj.org-article:040b3664ddca4d5fb50b3b3f8a5e83022021-11-18T07:16:27ZFine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon.1932-620310.1371/journal.pone.0038333https://doaj.org/article/040b3664ddca4d5fb50b3b3f8a5e83022012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22675544/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25 °C, whereas Bd21 is susceptible and infection results in an intense mosaic phenotype accompanied by high levels of replicating virus. We generated an F(6:7) recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21 and used the RILs, and an F(2) population of a second Bd21 × Bd3-1 cross to evaluate the inheritance of resistance. The results indicate that resistance segregates as expected for a single dominant gene, which we have designated Barley stripe mosaic virus resistance 1 (Bsr1). We constructed a genetic linkage map of the RIL population using SNP markers to map this gene to within 705 Kb of the distal end of the top of chromosome 3. Additional CAPS and Indel markers were used to fine map Bsr1 to a 23 Kb interval containing five putative genes. Our study demonstrates the power of using RILs to rapidly map the genetic determinants of BSMV resistance in Brachypodium. Moreover, the RILs and their associated genetic map, when combined with the complete genomic sequence of Brachypodium, provide new resources for genetic analyses of many other traits.Yu CuiMi Yeon LeeNaxin HuoJennifer BraggLijie YanCheng YuanCui LiSara J HolditchJingzhong XieMing-Cheng LuoDawei LiJialin YuJoel MartinWendy SchackwitzYong Qiang GuJohn P VogelAndrew O JacksonZhiyong LiuDavid F GarvinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38333 (2012) |
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Medicine R Science Q Yu Cui Mi Yeon Lee Naxin Huo Jennifer Bragg Lijie Yan Cheng Yuan Cui Li Sara J Holditch Jingzhong Xie Ming-Cheng Luo Dawei Li Jialin Yu Joel Martin Wendy Schackwitz Yong Qiang Gu John P Vogel Andrew O Jackson Zhiyong Liu David F Garvin Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon. |
| description |
The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25 °C, whereas Bd21 is susceptible and infection results in an intense mosaic phenotype accompanied by high levels of replicating virus. We generated an F(6:7) recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21 and used the RILs, and an F(2) population of a second Bd21 × Bd3-1 cross to evaluate the inheritance of resistance. The results indicate that resistance segregates as expected for a single dominant gene, which we have designated Barley stripe mosaic virus resistance 1 (Bsr1). We constructed a genetic linkage map of the RIL population using SNP markers to map this gene to within 705 Kb of the distal end of the top of chromosome 3. Additional CAPS and Indel markers were used to fine map Bsr1 to a 23 Kb interval containing five putative genes. Our study demonstrates the power of using RILs to rapidly map the genetic determinants of BSMV resistance in Brachypodium. Moreover, the RILs and their associated genetic map, when combined with the complete genomic sequence of Brachypodium, provide new resources for genetic analyses of many other traits. |
| format |
article |
| author |
Yu Cui Mi Yeon Lee Naxin Huo Jennifer Bragg Lijie Yan Cheng Yuan Cui Li Sara J Holditch Jingzhong Xie Ming-Cheng Luo Dawei Li Jialin Yu Joel Martin Wendy Schackwitz Yong Qiang Gu John P Vogel Andrew O Jackson Zhiyong Liu David F Garvin |
| author_facet |
Yu Cui Mi Yeon Lee Naxin Huo Jennifer Bragg Lijie Yan Cheng Yuan Cui Li Sara J Holditch Jingzhong Xie Ming-Cheng Luo Dawei Li Jialin Yu Joel Martin Wendy Schackwitz Yong Qiang Gu John P Vogel Andrew O Jackson Zhiyong Liu David F Garvin |
| author_sort |
Yu Cui |
| title |
Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon. |
| title_short |
Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon. |
| title_full |
Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon. |
| title_fullStr |
Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon. |
| title_full_unstemmed |
Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon. |
| title_sort |
fine mapping of the bsr1 barley stripe mosaic virus resistance gene in the model grass brachypodium distachyon. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/040b3664ddca4d5fb50b3b3f8a5e8302 |
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