Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins

Membrane proteins reside in the lipid bilayer of biomembranes and the structure and function of these proteins are closely related to their interactions with lipid molecules. Structural analyses of interactions between membrane proteins and lipids or detergents that constitute biological or artifici...

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Autores principales:	Shinya Hanashima, Takanori Nakane, Eiichi Mizohata
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	membrane protein lipid detergent chemical synthesis X-ray free-electron laser (XFEL) serial femtosecond crystallography (SFX) Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/040cdbe8bc71420197f593d2859ddc3e
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spelling	oai:doaj.org-article:040cdbe8bc71420197f593d2859ddc3e2021-11-25T18:19:37ZHeavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins10.3390/membranes111108232077-0375https://doaj.org/article/040cdbe8bc71420197f593d2859ddc3e2021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/823https://doaj.org/toc/2077-0375Membrane proteins reside in the lipid bilayer of biomembranes and the structure and function of these proteins are closely related to their interactions with lipid molecules. Structural analyses of interactions between membrane proteins and lipids or detergents that constitute biological or artificial model membranes are important for understanding the functions and physicochemical properties of membrane proteins and biomembranes. Determination of membrane protein structures is much more difficult when compared with that of soluble proteins, but the development of various new technologies has accelerated the elucidation of the structure-function relationship of membrane proteins. This review summarizes the development of heavy atom derivative detergents and lipids that can be used for structural analysis of membrane proteins and their interactions with detergents/lipids, including their application with X-ray free-electron laser crystallography.Shinya HanashimaTakanori NakaneEiichi MizohataMDPI AGarticlemembrane proteinlipiddetergentchemical synthesisX-ray free-electron laser (XFEL)serial femtosecond crystallography (SFX)Chemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 823, p 823 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	membrane protein lipid detergent chemical synthesis X-ray free-electron laser (XFEL) serial femtosecond crystallography (SFX) Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	membrane protein lipid detergent chemical synthesis X-ray free-electron laser (XFEL) serial femtosecond crystallography (SFX) Chemical technology TP1-1185 Chemical engineering TP155-156 Shinya Hanashima Takanori Nakane Eiichi Mizohata Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins
description	Membrane proteins reside in the lipid bilayer of biomembranes and the structure and function of these proteins are closely related to their interactions with lipid molecules. Structural analyses of interactions between membrane proteins and lipids or detergents that constitute biological or artificial model membranes are important for understanding the functions and physicochemical properties of membrane proteins and biomembranes. Determination of membrane protein structures is much more difficult when compared with that of soluble proteins, but the development of various new technologies has accelerated the elucidation of the structure-function relationship of membrane proteins. This review summarizes the development of heavy atom derivative detergents and lipids that can be used for structural analysis of membrane proteins and their interactions with detergents/lipids, including their application with X-ray free-electron laser crystallography.
format	article
author	Shinya Hanashima Takanori Nakane Eiichi Mizohata
author_facet	Shinya Hanashima Takanori Nakane Eiichi Mizohata
author_sort	Shinya Hanashima
title	Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins
title_short	Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins
title_full	Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins
title_fullStr	Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins
title_full_unstemmed	Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins
title_sort	heavy atom detergent/lipid combined x-ray crystallography for elucidating the structure-function relationships of membrane proteins
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/040cdbe8bc71420197f593d2859ddc3e
work_keys_str_mv	AT shinyahanashima heavyatomdetergentlipidcombinedxraycrystallographyforelucidatingthestructurefunctionrelationshipsofmembraneproteins AT takanorinakane heavyatomdetergentlipidcombinedxraycrystallographyforelucidatingthestructurefunctionrelationshipsofmembraneproteins AT eiichimizohata heavyatomdetergentlipidcombinedxraycrystallographyforelucidatingthestructurefunctionrelationshipsofmembraneproteins
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Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins

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