Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Abstract Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, some cell-cell interactions are limited by the existing hydrogel. To better mimic tumor microenvironment, self-assembled multicellular heterogeneous brain tumor fibers h...

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Autores principales:	Xingliang Dai, Libiao Liu, Jia Ouyang, Xinda Li, Xinzhi Zhang, Qing Lan, Tao Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/04134255ebb740dc92e3b0c9ef926cae
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spelling	oai:doaj.org-article:04134255ebb740dc92e3b0c9ef926cae2021-12-02T11:41:01ZCoaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers10.1038/s41598-017-01581-y2045-2322https://doaj.org/article/04134255ebb740dc92e3b0c9ef926cae2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01581-yhttps://doaj.org/toc/2045-2322Abstract Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, some cell-cell interactions are limited by the existing hydrogel. To better mimic tumor microenvironment, self-assembled multicellular heterogeneous brain tumor fibers have been fabricated by a custom-made coaxial extrusion 3D bioprinting system, with high viability, proliferative activity and efficient tumor-stromal interactions. Therein, in order to further verify the sufficient interactions between tumor cells and stroma MSCs, CRE-LOXP switch gene system which contained GSCs transfected with “LOXP-STOP-LOXP-RFP” genes and MSCs transfected with “CRE recombinase” gene was used. Results showed that tumor-stroma cells interacted with each other and fused, the transcription of RFP was higher than that of 2D culture model and control group with cells mixed directly into alginate, respectively. RFP expression was observed only in the cell fibers but not in the control group under confocal microscope. In conclusion, coaxial 3D bioprinted multicellular self-assembled heterogeneous tumor tissue-like fibers provided preferable 3D models for studying tumor microenvironment in vitro, especially for tumor-stromal interactions.Xingliang DaiLibiao LiuJia OuyangXinda LiXinzhi ZhangQing LanTao XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xingliang Dai Libiao Liu Jia Ouyang Xinda Li Xinzhi Zhang Qing Lan Tao Xu Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
description	Abstract Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, some cell-cell interactions are limited by the existing hydrogel. To better mimic tumor microenvironment, self-assembled multicellular heterogeneous brain tumor fibers have been fabricated by a custom-made coaxial extrusion 3D bioprinting system, with high viability, proliferative activity and efficient tumor-stromal interactions. Therein, in order to further verify the sufficient interactions between tumor cells and stroma MSCs, CRE-LOXP switch gene system which contained GSCs transfected with “LOXP-STOP-LOXP-RFP” genes and MSCs transfected with “CRE recombinase” gene was used. Results showed that tumor-stroma cells interacted with each other and fused, the transcription of RFP was higher than that of 2D culture model and control group with cells mixed directly into alginate, respectively. RFP expression was observed only in the cell fibers but not in the control group under confocal microscope. In conclusion, coaxial 3D bioprinted multicellular self-assembled heterogeneous tumor tissue-like fibers provided preferable 3D models for studying tumor microenvironment in vitro, especially for tumor-stromal interactions.
format	article
author	Xingliang Dai Libiao Liu Jia Ouyang Xinda Li Xinzhi Zhang Qing Lan Tao Xu
author_facet	Xingliang Dai Libiao Liu Jia Ouyang Xinda Li Xinzhi Zhang Qing Lan Tao Xu
author_sort	Xingliang Dai
title	Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
title_short	Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
title_full	Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
title_fullStr	Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
title_full_unstemmed	Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers
title_sort	coaxial 3d bioprinting of self-assembled multicellular heterogeneous tumor fibers
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/04134255ebb740dc92e3b0c9ef926cae
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_version_	1718395440644227072

Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

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