The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water

Abstract Surviving the nutrient-poor aquatic environment for extended periods of time is important for the transmission of various water-borne pathogens, including Legionella pneumophila (Lp). Previous work concluded that the stringent response and the sigma factor RpoS are essential for the surviva...

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Autores principales:	Nilmini Mendis, Peter McBride, Joseph Saoud, Thangadurai Mani, Sebastien P. Faucher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/041ada4e1a704882b6a2c60f3dd375e8
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spelling	oai:doaj.org-article:041ada4e1a704882b6a2c60f3dd375e82021-12-02T12:31:56ZThe LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water10.1038/s41598-018-24263-92045-2322https://doaj.org/article/041ada4e1a704882b6a2c60f3dd375e82018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24263-9https://doaj.org/toc/2045-2322Abstract Surviving the nutrient-poor aquatic environment for extended periods of time is important for the transmission of various water-borne pathogens, including Legionella pneumophila (Lp). Previous work concluded that the stringent response and the sigma factor RpoS are essential for the survival of Lp in water. In the present study, we investigated the role of the LetA/S two-component signal transduction system in the successful survival of Lp in water. In addition to cell size reduction in the post-exponential phase, LetS also contributes to cell size reduction when Lp is exposed to water. Importantly, absence of the sensor kinase results in a significantly lower survival as measured by CFUs in water at various temperatures and an increased sensitivity to heat shock. According to the transcriptomic analysis, LetA/S orchestrates a general transcriptomic downshift of major metabolic pathways upon exposure to water leading to better culturability, and likely survival, suggesting a potential link with the stringent response. However, the expression of the LetA/S regulated small regulatory RNAs, RsmY and RsmZ, is not changed in a relAspoT mutant, which indicates that the stringent response and the LetA/S response are two distinct regulatory systems contributing to the survival of Lp in water.Nilmini MendisPeter McBrideJoseph SaoudThangadurai ManiSebastien P. FaucherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-17 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nilmini Mendis Peter McBride Joseph Saoud Thangadurai Mani Sebastien P. Faucher The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water
description	Abstract Surviving the nutrient-poor aquatic environment for extended periods of time is important for the transmission of various water-borne pathogens, including Legionella pneumophila (Lp). Previous work concluded that the stringent response and the sigma factor RpoS are essential for the survival of Lp in water. In the present study, we investigated the role of the LetA/S two-component signal transduction system in the successful survival of Lp in water. In addition to cell size reduction in the post-exponential phase, LetS also contributes to cell size reduction when Lp is exposed to water. Importantly, absence of the sensor kinase results in a significantly lower survival as measured by CFUs in water at various temperatures and an increased sensitivity to heat shock. According to the transcriptomic analysis, LetA/S orchestrates a general transcriptomic downshift of major metabolic pathways upon exposure to water leading to better culturability, and likely survival, suggesting a potential link with the stringent response. However, the expression of the LetA/S regulated small regulatory RNAs, RsmY and RsmZ, is not changed in a relAspoT mutant, which indicates that the stringent response and the LetA/S response are two distinct regulatory systems contributing to the survival of Lp in water.
format	article
author	Nilmini Mendis Peter McBride Joseph Saoud Thangadurai Mani Sebastien P. Faucher
author_facet	Nilmini Mendis Peter McBride Joseph Saoud Thangadurai Mani Sebastien P. Faucher
author_sort	Nilmini Mendis
title	The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water
title_short	The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water
title_full	The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water
title_fullStr	The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water
title_full_unstemmed	The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water
title_sort	leta/s two-component system regulates transcriptomic changes that are essential for the culturability of legionella pneumophila in water
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/041ada4e1a704882b6a2c60f3dd375e8
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The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water

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