Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>

Chloroplasts are semi-autonomous organelles governed by the precise coordination between the genomes of their own and the nucleus for functioning correctly in response to developmental and environmental cues. Under stressed conditions, various plastid-to-nucleus retrograde signals are generated to r...

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Autores principales:	Linjuan Wang, Xingqi Huang, Kui Li, Shuyuan Song, Yunhe Jing, Shan Lu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chloroplast DJC31 GUN1 HCF145 protein–protein interaction retrograde signal Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/041b6cbe66ff48a290828b26ced05909
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spelling	oai:doaj.org-article:041b6cbe66ff48a290828b26ced059092021-11-11T16:50:18ZScreening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>10.3390/ijms2221113641422-00671661-6596https://doaj.org/article/041b6cbe66ff48a290828b26ced059092021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11364https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Chloroplasts are semi-autonomous organelles governed by the precise coordination between the genomes of their own and the nucleus for functioning correctly in response to developmental and environmental cues. Under stressed conditions, various plastid-to-nucleus retrograde signals are generated to regulate the expression of a large number of nuclear genes for acclimation. Among these retrograde signaling pathways, the chloroplast protein GENOMES UNCOUPLED 1 (GUN1) is the first component identified. However, in addition to integrating aberrant physiological signals when chloroplasts are challenged by stresses such as photooxidative damage or the inhibition of plastid gene expression, GUN1 was also found to regulate other developmental processes such as flowering. Several partner proteins have been found to interact with GUN1 and facilitate its different regulatory functions. In this study, we report 15 possible interacting proteins identified through yeast two-hybrid (Y2H) screening, among which 11 showed positive interactions by pair-wise Y2H assay. Through the bimolecular fluorescence complementation assay in Arabidopsis protoplasts, two candidate proteins with chloroplast localization, DJC31 and HCF145, were confirmed to interact with GUN1 <i>in planta.</i> Genes for these GUN1-interacting proteins showed different fluctuations in the WT and <i>gun1</i> mutant under norflurazon and lincomycin treatments. Our results provide novel clues for a better understanding of molecular mechanisms underlying GUN1-mediated regulations.Linjuan WangXingqi HuangKui LiShuyuan SongYunhe JingShan LuMDPI AGarticlechloroplastDJC31GUN1HCF145protein–protein interactionretrograde signalBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11364, p 11364 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chloroplast DJC31 GUN1 HCF145 protein–protein interaction retrograde signal Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	chloroplast DJC31 GUN1 HCF145 protein–protein interaction retrograde signal Biology (General) QH301-705.5 Chemistry QD1-999 Linjuan Wang Xingqi Huang Kui Li Shuyuan Song Yunhe Jing Shan Lu Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>
description	Chloroplasts are semi-autonomous organelles governed by the precise coordination between the genomes of their own and the nucleus for functioning correctly in response to developmental and environmental cues. Under stressed conditions, various plastid-to-nucleus retrograde signals are generated to regulate the expression of a large number of nuclear genes for acclimation. Among these retrograde signaling pathways, the chloroplast protein GENOMES UNCOUPLED 1 (GUN1) is the first component identified. However, in addition to integrating aberrant physiological signals when chloroplasts are challenged by stresses such as photooxidative damage or the inhibition of plastid gene expression, GUN1 was also found to regulate other developmental processes such as flowering. Several partner proteins have been found to interact with GUN1 and facilitate its different regulatory functions. In this study, we report 15 possible interacting proteins identified through yeast two-hybrid (Y2H) screening, among which 11 showed positive interactions by pair-wise Y2H assay. Through the bimolecular fluorescence complementation assay in Arabidopsis protoplasts, two candidate proteins with chloroplast localization, DJC31 and HCF145, were confirmed to interact with GUN1 <i>in planta.</i> Genes for these GUN1-interacting proteins showed different fluctuations in the WT and <i>gun1</i> mutant under norflurazon and lincomycin treatments. Our results provide novel clues for a better understanding of molecular mechanisms underlying GUN1-mediated regulations.
format	article
author	Linjuan Wang Xingqi Huang Kui Li Shuyuan Song Yunhe Jing Shan Lu
author_facet	Linjuan Wang Xingqi Huang Kui Li Shuyuan Song Yunhe Jing Shan Lu
author_sort	Linjuan Wang
title	Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>
title_short	Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>
title_full	Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>
title_fullStr	Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>
title_full_unstemmed	Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>
title_sort	screening and identification of candidate gun1-interacting proteins in <i>arabidopsis thaliana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/041b6cbe66ff48a290828b26ced05909
work_keys_str_mv	AT linjuanwang screeningandidentificationofcandidategun1interactingproteinsiniarabidopsisthalianai AT xingqihuang screeningandidentificationofcandidategun1interactingproteinsiniarabidopsisthalianai AT kuili screeningandidentificationofcandidategun1interactingproteinsiniarabidopsisthalianai AT shuyuansong screeningandidentificationofcandidategun1interactingproteinsiniarabidopsisthalianai AT yunhejing screeningandidentificationofcandidategun1interactingproteinsiniarabidopsisthalianai AT shanlu screeningandidentificationofcandidategun1interactingproteinsiniarabidopsisthalianai
_version_	1718432219488321536

Screening and Identification of Candidate GUN1-Interacting Proteins in <i>Arabidopsis thaliana</i>

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