Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles

Mathias K Gehrmann,1 Melanie A Kimm,2 Stefan Stangl,1 Thomas E Schmid,1 Peter B Noël,2 Ernst J Rummeny,2 Gabriele Multhoff11Department of Radiation Oncology, 2Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technische Universität München, M...

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Autores principales:	Gehrmann MK, Kimm MA, Stangl S, Schmid TE, Noël PB, Rummeny EJ, Multhoff G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0426357c5d5a4c6a84f857bd2bd6c0d1
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spelling	oai:doaj.org-article:0426357c5d5a4c6a84f857bd2bd6c0d12021-12-02T03:58:28ZImaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles1178-2013https://doaj.org/article/0426357c5d5a4c6a84f857bd2bd6c0d12015-09-01T00:00:00Zhttps://www.dovepress.com/imaging-of-hsp70-positive-tumors-with-cmhsp701-antibody-conjugated-gol-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mathias K Gehrmann,1 Melanie A Kimm,2 Stefan Stangl,1 Thomas E Schmid,1 Peter B Noël,2 Ernst J Rummeny,2 Gabriele Multhoff11Department of Radiation Oncology, 2Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, GermanyAbstract: Real-time imaging of small tumors is still one of the challenges in cancer diagnosis, prognosis, and monitoring of clinical outcome. Targeting novel biomarkers that are selectively expressed on a large variety of different tumors but not normal cells has the potential to improve the imaging capacity of existing methods such as computed tomography. Herein, we present a novel technique using cmHsp70.1 monoclonal antibody-conjugated spherical gold nanoparticles for quantification of the targeted uptake of gold nanoparticles into membrane Hsp70-positive tumor cells. Upon binding, cmHsp70.1-conjugated gold nanoparticles but not nanoparticles coupled to an isotype-matched IgG1 antibody or empty nanoparticles are rapidly taken up by highly malignant Hsp70 membrane-positive mouse tumor cells. After 24 hours, the cmHsp70.1-conjugated gold nanoparticles are found to be enriched in the perinuclear region. Specificity for membrane Hsp70 was shown by using an Hsp70 knockout tumor cell system. Toxic side effects of the cmHsp70.1-conjugated nanoparticles are not observed at a concentration of 1–10 µg/mL. Experiments are ongoing to evaluate whether cmHsp70.1 antibody-conjugated gold nanoparticles are suitable for the detection of membrane-Hsp70-positive tumors in vivo.Keywords: heat shock protein 70, tumor biomarker, theranostics, multimodal CT, multispectral CT, k-edgeGehrmann MKKimm MAStangl SSchmid TENoël PBRummeny EJMulthoff GDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5687-5700 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Gehrmann MK Kimm MA Stangl S Schmid TE Noël PB Rummeny EJ Multhoff G Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles
description	Mathias K Gehrmann,1 Melanie A Kimm,2 Stefan Stangl,1 Thomas E Schmid,1 Peter B Noël,2 Ernst J Rummeny,2 Gabriele Multhoff11Department of Radiation Oncology, 2Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, GermanyAbstract: Real-time imaging of small tumors is still one of the challenges in cancer diagnosis, prognosis, and monitoring of clinical outcome. Targeting novel biomarkers that are selectively expressed on a large variety of different tumors but not normal cells has the potential to improve the imaging capacity of existing methods such as computed tomography. Herein, we present a novel technique using cmHsp70.1 monoclonal antibody-conjugated spherical gold nanoparticles for quantification of the targeted uptake of gold nanoparticles into membrane Hsp70-positive tumor cells. Upon binding, cmHsp70.1-conjugated gold nanoparticles but not nanoparticles coupled to an isotype-matched IgG1 antibody or empty nanoparticles are rapidly taken up by highly malignant Hsp70 membrane-positive mouse tumor cells. After 24 hours, the cmHsp70.1-conjugated gold nanoparticles are found to be enriched in the perinuclear region. Specificity for membrane Hsp70 was shown by using an Hsp70 knockout tumor cell system. Toxic side effects of the cmHsp70.1-conjugated nanoparticles are not observed at a concentration of 1–10 µg/mL. Experiments are ongoing to evaluate whether cmHsp70.1 antibody-conjugated gold nanoparticles are suitable for the detection of membrane-Hsp70-positive tumors in vivo.Keywords: heat shock protein 70, tumor biomarker, theranostics, multimodal CT, multispectral CT, k-edge
format	article
author	Gehrmann MK Kimm MA Stangl S Schmid TE Noël PB Rummeny EJ Multhoff G
author_facet	Gehrmann MK Kimm MA Stangl S Schmid TE Noël PB Rummeny EJ Multhoff G
author_sort	Gehrmann MK
title	Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles
title_short	Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles
title_full	Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles
title_fullStr	Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles
title_full_unstemmed	Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles
title_sort	imaging of hsp70-positive tumors with cmhsp70.1 antibody-conjugated gold nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/0426357c5d5a4c6a84f857bd2bd6c0d1
work_keys_str_mv	AT gehrmannmk imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles AT kimmma imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles AT stangls imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles AT schmidte imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles AT noeumllpb imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles AT rummenyej imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles AT multhoffg imagingofhsp70positivetumorswithcmhsp701antibodyconjugatedgoldnanoparticles
_version_	1718401470356783104

Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles

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