Distribution of SiO2 nanoparticles in 3D liver microtissues

Jana Fleddermann,1 Julia Susewind,2 Henrike Peuschel,1 Marcus Koch,1 Isabella Tavernaro,1 Annette Kraegeloh1 1INM – Leibniz Institute for New Materials, Saarbrücken, Germany; 2Pharmacelsus GmbH, Saarbrücken, Germany Introduction: Nanoparticles (NPs) are used in numerous...

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Autores principales:	Fleddermann J, Susewind J, Peuschel H, Koch M, Tavernaro I, Kraegeloh A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Silica nanoparticles Human hepatocarcinoma cells Spheroids Penetration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/04273b4c5e9f4b4397df4a903d6c6612
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spelling	oai:doaj.org-article:04273b4c5e9f4b4397df4a903d6c66122021-12-02T07:14:39ZDistribution of SiO2 nanoparticles in 3D liver microtissues1178-2013https://doaj.org/article/04273b4c5e9f4b4397df4a903d6c66122019-02-01T00:00:00Zhttps://www.dovepress.com/distribution-of-sio2-nanoparticles-in-3d-liver-microtissues-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jana Fleddermann,1 Julia Susewind,2 Henrike Peuschel,1 Marcus Koch,1 Isabella Tavernaro,1 Annette Kraegeloh1 1INM – Leibniz Institute for New Materials, Saarbrücken, Germany; 2Pharmacelsus GmbH, Saarbrücken, Germany Introduction: Nanoparticles (NPs) are used in numerous products in technical fields and biomedicine; their potential adverse effects have to be considered in order to achieve safe applications. Besides their distribution in tissues, organs, and cellular localization, their impact and penetration during the process of tissue formation occurring in vivo during liver regeneration are critical steps for establishment of safe nanomaterials.Materials and methods: In this study, 3D cell culture of human hepatocarcinoma cells (HepG2) was used to generate cellular spheroids, serving as in vitro liver microtissues. In order to determine their differential distribution and penetration depth in HepG2 spheroids, SiO2 NPs were applied either during or after spheroid formation. The NP penetration was comprehensively studied using confocal laser scanning microscopy and scanning electron microscopy.Results: Spheroids were exposed to 100 µg mL-1 SiO2 NPs either at the beginning of spheroid formation, or during or after formation of spheroids. Microscopy analyses revealed that NP penetration into the spheroid is limited. During and after spheroid formation, SiO2 NPs penetrated about 20 µm into the spheroids, corresponding to about three cell layers. In contrast, because of the addition of SiO2 NPs simultaneously to cell seeding, NP agglomerates were located also in the spheroid center. Application of SiO2 NPs during the process of spheroid formation had no impact on final spheroid size.Conclusion: Understanding the distribution of NPs in tissues is essential for biomedical applications. The obtained results indicate that NPs show only limited penetration into already formed tissue, which is probably caused by the alteration of the tissue structure and cell packing density during the process of spheroid formation. Keywords: silica nanoparticles, human hepatocarcinoma cells, spheroids, penetration  Fleddermann JSusewind JPeuschel HKoch MTavernaro IKraegeloh ADove Medical PressarticleSilica nanoparticlesHuman hepatocarcinoma cellsSpheroidsPenetrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1411-1431 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silica nanoparticles Human hepatocarcinoma cells Spheroids Penetration Medicine (General) R5-920
spellingShingle	Silica nanoparticles Human hepatocarcinoma cells Spheroids Penetration Medicine (General) R5-920 Fleddermann J Susewind J Peuschel H Koch M Tavernaro I Kraegeloh A Distribution of SiO2 nanoparticles in 3D liver microtissues
description	Jana Fleddermann,1 Julia Susewind,2 Henrike Peuschel,1 Marcus Koch,1 Isabella Tavernaro,1 Annette Kraegeloh1 1INM – Leibniz Institute for New Materials, Saarbrücken, Germany; 2Pharmacelsus GmbH, Saarbrücken, Germany Introduction: Nanoparticles (NPs) are used in numerous products in technical fields and biomedicine; their potential adverse effects have to be considered in order to achieve safe applications. Besides their distribution in tissues, organs, and cellular localization, their impact and penetration during the process of tissue formation occurring in vivo during liver regeneration are critical steps for establishment of safe nanomaterials.Materials and methods: In this study, 3D cell culture of human hepatocarcinoma cells (HepG2) was used to generate cellular spheroids, serving as in vitro liver microtissues. In order to determine their differential distribution and penetration depth in HepG2 spheroids, SiO2 NPs were applied either during or after spheroid formation. The NP penetration was comprehensively studied using confocal laser scanning microscopy and scanning electron microscopy.Results: Spheroids were exposed to 100 µg mL-1 SiO2 NPs either at the beginning of spheroid formation, or during or after formation of spheroids. Microscopy analyses revealed that NP penetration into the spheroid is limited. During and after spheroid formation, SiO2 NPs penetrated about 20 µm into the spheroids, corresponding to about three cell layers. In contrast, because of the addition of SiO2 NPs simultaneously to cell seeding, NP agglomerates were located also in the spheroid center. Application of SiO2 NPs during the process of spheroid formation had no impact on final spheroid size.Conclusion: Understanding the distribution of NPs in tissues is essential for biomedical applications. The obtained results indicate that NPs show only limited penetration into already formed tissue, which is probably caused by the alteration of the tissue structure and cell packing density during the process of spheroid formation. Keywords: silica nanoparticles, human hepatocarcinoma cells, spheroids, penetration
format	article
author	Fleddermann J Susewind J Peuschel H Koch M Tavernaro I Kraegeloh A
author_facet	Fleddermann J Susewind J Peuschel H Koch M Tavernaro I Kraegeloh A
author_sort	Fleddermann J
title	Distribution of SiO2 nanoparticles in 3D liver microtissues
title_short	Distribution of SiO2 nanoparticles in 3D liver microtissues
title_full	Distribution of SiO2 nanoparticles in 3D liver microtissues
title_fullStr	Distribution of SiO2 nanoparticles in 3D liver microtissues
title_full_unstemmed	Distribution of SiO2 nanoparticles in 3D liver microtissues
title_sort	distribution of sio2 nanoparticles in 3d liver microtissues
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/04273b4c5e9f4b4397df4a903d6c6612
work_keys_str_mv	AT fleddermannj distributionofsio2nbspnanoparticlesnbspin3dlivermicrotissues AT susewindj distributionofsio2nbspnanoparticlesnbspin3dlivermicrotissues AT peuschelh distributionofsio2nbspnanoparticlesnbspin3dlivermicrotissues AT kochm distributionofsio2nbspnanoparticlesnbspin3dlivermicrotissues AT tavernaroi distributionofsio2nbspnanoparticlesnbspin3dlivermicrotissues AT kraegeloha distributionofsio2nbspnanoparticlesnbspin3dlivermicrotissues
_version_	1718399497745203200

Distribution of SiO2&nbsp;nanoparticles&nbsp;in 3D liver microtissues

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Distribution of SiO2 nanoparticles in 3D liver microtissues