Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy

Abstract In this study, we used spectrally focused coherent anti-Stokes Raman scattering (spCARS) microscopy assisted by sum-frequency generation (SFG) to monitor the variations in the structural morphology and molecular vibrations of a live muscle of Caenorhabditis elegans. The subunits of the musc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyunmin Kim, Do-Young Kim, Kyung-Il Joo, Jung-Hye Kim, Soon Moon Jeong, Eun Seong Lee, Jeong-Hoon Hahm, Kyuhyung Kim, Dae Woon Moon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/042d4f3ce822433ea36642d0d928f9a0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:042d4f3ce822433ea36642d0d928f9a0
record_format	dspace
spelling	oai:doaj.org-article:042d4f3ce822433ea36642d0d928f9a02021-12-02T15:04:58ZCoherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy10.1038/s41598-017-09571-w2045-2322https://doaj.org/article/042d4f3ce822433ea36642d0d928f9a02017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09571-whttps://doaj.org/toc/2045-2322Abstract In this study, we used spectrally focused coherent anti-Stokes Raman scattering (spCARS) microscopy assisted by sum-frequency generation (SFG) to monitor the variations in the structural morphology and molecular vibrations of a live muscle of Caenorhabditis elegans. The subunits of the muscle sarcomeres, such as the M-line, myosin, dense body, and α-actinin, were alternatively observed using spCARS microscopy for different sample orientations, with the guidance of a myosin positional marker captured by SFG microscopy. Interestingly enough, the beam polarization dependence of the spCARS contrasts for two parallel subunits (dense body and myosin) showed a ~90° phase difference. The chemically sensitive spCARS spectra induced by the time-varying overlap of two pulses allowed (after a robust subtraction of the non-resonant background using a modified Kramers–Krönig transformation method) high-fidelity detection of various genetically modified muscle sarcomeres tuned to the C-H vibration (2800–3100 cm−1). Conversely, SFG image mapping assisted by phase-retrieved spCARS spectra also facilitated label-free monitoring of the changes in the muscle content of C. elegans that are associated with aging, based on the hypothesis that the C-H vibrational modes could serve as qualitative chemical markers sensitive to the amount and/or structural modulation of the muscle.Hyunmin KimDo-Young KimKyung-Il JooJung-Hye KimSoon Moon JeongEun Seong LeeJeong-Hoon HahmKyuhyung KimDae Woon MoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyunmin Kim Do-Young Kim Kyung-Il Joo Jung-Hye Kim Soon Moon Jeong Eun Seong Lee Jeong-Hoon Hahm Kyuhyung Kim Dae Woon Moon Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy
description	Abstract In this study, we used spectrally focused coherent anti-Stokes Raman scattering (spCARS) microscopy assisted by sum-frequency generation (SFG) to monitor the variations in the structural morphology and molecular vibrations of a live muscle of Caenorhabditis elegans. The subunits of the muscle sarcomeres, such as the M-line, myosin, dense body, and α-actinin, were alternatively observed using spCARS microscopy for different sample orientations, with the guidance of a myosin positional marker captured by SFG microscopy. Interestingly enough, the beam polarization dependence of the spCARS contrasts for two parallel subunits (dense body and myosin) showed a ~90° phase difference. The chemically sensitive spCARS spectra induced by the time-varying overlap of two pulses allowed (after a robust subtraction of the non-resonant background using a modified Kramers–Krönig transformation method) high-fidelity detection of various genetically modified muscle sarcomeres tuned to the C-H vibration (2800–3100 cm−1). Conversely, SFG image mapping assisted by phase-retrieved spCARS spectra also facilitated label-free monitoring of the changes in the muscle content of C. elegans that are associated with aging, based on the hypothesis that the C-H vibrational modes could serve as qualitative chemical markers sensitive to the amount and/or structural modulation of the muscle.
format	article
author	Hyunmin Kim Do-Young Kim Kyung-Il Joo Jung-Hye Kim Soon Moon Jeong Eun Seong Lee Jeong-Hoon Hahm Kyuhyung Kim Dae Woon Moon
author_facet	Hyunmin Kim Do-Young Kim Kyung-Il Joo Jung-Hye Kim Soon Moon Jeong Eun Seong Lee Jeong-Hoon Hahm Kyuhyung Kim Dae Woon Moon
author_sort	Hyunmin Kim
title	Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy
title_short	Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy
title_full	Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy
title_fullStr	Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy
title_full_unstemmed	Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy
title_sort	coherent raman imaging of live muscle sarcomeres assisted by sfg microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/042d4f3ce822433ea36642d0d928f9a0
work_keys_str_mv	AT hyunminkim coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT doyoungkim coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT kyungiljoo coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT junghyekim coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT soonmoonjeong coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT eunseonglee coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT jeonghoonhahm coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT kyuhyungkim coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy AT daewoonmoon coherentramanimagingoflivemusclesarcomeresassistedbysfgmicroscopy
_version_	1718388933904039936

Coherent Raman Imaging of Live Muscle Sarcomeres Assisted by SFG Microscopy

Ejemplares similares