Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer

Abstract Recent advancements in the field of immune-oncology have led to a significant increase in life expectancy of patients with diverse forms of cancer, such as hematologic malignancies, melanoma and lung cancer. Unfortunately, these encouraging results are not observed in the majority of patien...

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Autores principales:	Q. Lecocq, P. Debie, J. Puttemans, R. M. Awad, L. De Beck, T. Ertveldt, Y. De Vlaeminck, C. Goyvaerts, G. Raes, M. Keyaerts, K. Breckpot, N. Devoogdt
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Immunotherapy Immune checkpoint Lymphocyte activation gene-3 Single-domain antibody Nanobody Molecular imaging Medical physics. Medical radiology. Nuclear medicine R895-920
Acceso en línea:	https://doaj.org/article/0430de807ba24283850dbe2c50c599d5
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spelling	oai:doaj.org-article:0430de807ba24283850dbe2c50c599d52021-11-08T10:45:46ZEvaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer10.1186/s13550-021-00857-92191-219Xhttps://doaj.org/article/0430de807ba24283850dbe2c50c599d52021-11-01T00:00:00Zhttps://doi.org/10.1186/s13550-021-00857-9https://doaj.org/toc/2191-219XAbstract Recent advancements in the field of immune-oncology have led to a significant increase in life expectancy of patients with diverse forms of cancer, such as hematologic malignancies, melanoma and lung cancer. Unfortunately, these encouraging results are not observed in the majority of patients, who remain unresponsive and/or encounter adverse events. Currently, researchers are collecting more insight into the cellular and molecular mechanisms that underlie these variable responses. As an example, the human lymphocyte activation gene-3 (huLAG-3), an inhibitory immune checkpoint receptor, is increasingly studied as a therapeutic target in immune-oncology. Noninvasive molecular imaging of the immune checkpoint programmed death protein-1 (PD-1) or its ligand PD-L1 has shown its value as a strategy to guide and monitor PD-1/PD-L1-targeted immune checkpoint therapy. Yet, radiotracers that allow dynamic, whole body imaging of huLAG-3 expression are not yet described. We here developed single-domain antibodies (sdAbs) that bind huLAG-3 and showed that these sdAbs can image huLAG-3 in tumors, therefore representing promising tools for further development into clinically applicable radiotracers.Q. LecocqP. DebieJ. PuttemansR. M. AwadL. De BeckT. ErtveldtY. De VlaeminckC. GoyvaertsG. RaesM. KeyaertsK. BreckpotN. DevoogdtSpringerOpenarticleImmunotherapyImmune checkpointLymphocyte activation gene-3Single-domain antibodyNanobodyMolecular imagingMedical physics. Medical radiology. Nuclear medicineR895-920ENEJNMMI Research, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunotherapy Immune checkpoint Lymphocyte activation gene-3 Single-domain antibody Nanobody Molecular imaging Medical physics. Medical radiology. Nuclear medicine R895-920
spellingShingle	Immunotherapy Immune checkpoint Lymphocyte activation gene-3 Single-domain antibody Nanobody Molecular imaging Medical physics. Medical radiology. Nuclear medicine R895-920 Q. Lecocq P. Debie J. Puttemans R. M. Awad L. De Beck T. Ertveldt Y. De Vlaeminck C. Goyvaerts G. Raes M. Keyaerts K. Breckpot N. Devoogdt Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
description	Abstract Recent advancements in the field of immune-oncology have led to a significant increase in life expectancy of patients with diverse forms of cancer, such as hematologic malignancies, melanoma and lung cancer. Unfortunately, these encouraging results are not observed in the majority of patients, who remain unresponsive and/or encounter adverse events. Currently, researchers are collecting more insight into the cellular and molecular mechanisms that underlie these variable responses. As an example, the human lymphocyte activation gene-3 (huLAG-3), an inhibitory immune checkpoint receptor, is increasingly studied as a therapeutic target in immune-oncology. Noninvasive molecular imaging of the immune checkpoint programmed death protein-1 (PD-1) or its ligand PD-L1 has shown its value as a strategy to guide and monitor PD-1/PD-L1-targeted immune checkpoint therapy. Yet, radiotracers that allow dynamic, whole body imaging of huLAG-3 expression are not yet described. We here developed single-domain antibodies (sdAbs) that bind huLAG-3 and showed that these sdAbs can image huLAG-3 in tumors, therefore representing promising tools for further development into clinically applicable radiotracers.
format	article
author	Q. Lecocq P. Debie J. Puttemans R. M. Awad L. De Beck T. Ertveldt Y. De Vlaeminck C. Goyvaerts G. Raes M. Keyaerts K. Breckpot N. Devoogdt
author_facet	Q. Lecocq P. Debie J. Puttemans R. M. Awad L. De Beck T. Ertveldt Y. De Vlaeminck C. Goyvaerts G. Raes M. Keyaerts K. Breckpot N. Devoogdt
author_sort	Q. Lecocq
title	Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
title_short	Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
title_full	Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
title_fullStr	Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
title_full_unstemmed	Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
title_sort	evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/0430de807ba24283850dbe2c50c599d5
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Evaluation of single domain antibodies as nuclear tracers for imaging of the immune checkpoint receptor human lymphocyte activation gene-3 in cancer

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